Genetics and Genomics of Abiotic Stress in Rubber Tree (Hevea Brasiliensis)

Rekha, K.; Jayasree, Parukuttyamma Kumari; Rout, Jayashree; Uthup, Thomas K.; Mathew, Suni Annie; Sathik, M. B. Mohamed

doi:10.1007/978-3-031-05706-9_8

Cited by 3 publications

(2 citation statements)

References 160 publications

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“…Agrobacterium -mediated genetic transformation serves as a powerful tool in targeted modification of various crops, including the important industrial tropical crop species, H. brasiliensis . However, H. brasiliensis is recalcitrant to genetic transformation, and only a few clones (varieties) have so far been reported to be successfully transformed [ 41 ]. Low regeneration rates of somatic embryos and high genotype-dependence are the major limiting factors that hinder the efficient application of transgenic technique in rubber breeding.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of HbGRF4 or HbGRF4-HbGIF1 Chimera Improves the Efficiency of Somatic Embryogenesis in Hevea brasiliensis

Luo,

Zhang,

Zhou

et al. 2024

IJMS

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Transgenic technology is a crucial tool for gene functional analysis and targeted genetic modification in the para rubber tree (Hevea brasiliensis). However, low efficiency of plant regeneration via somatic embryogenesis remains a bottleneck of successful genetic transformation in H. brasiliensis. Enhancing expression of GROWTH-REGULATING FACTOR 4 (GRF4)-GRF-INTERACTING FACTOR 1 (GIF1) has been reported to significantly improve shoot and embryo regeneration in multiple crops. Here, we identified endogenous HbGRF4 and HbGIF1 from the rubber clone Reyan7-33-97, the expressions of which dramatically increased along with somatic embryo (SE) production. Intriguingly, overexpression of HbGRF4 or HbGRF4-HbGIF1 markedly enhanced the efficiency of embryogenesis in two H. brasiliensis callus lines with contrasting rates of SE production. Transcriptional profiling revealed that the genes involved in jasmonic acid response were up-regulated, whereas those in ethylene biosynthesis and response as well as the S-adenosylmethionine-dependent methyltransferase activity were down-regulated in HbGRF4- and HbGRF4-HbGIF1-overexpressing H. brasiliensis embryos. These findings open up a new avenue for improving SE production in rubber tree, and help to unravel the underlying mechanisms of HbGRF4-enhanced somatic embryogenesis.

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Section: Discussionmentioning

confidence: 99%

Overexpression of HbGRF4 or HbGRF4-HbGIF1 Chimera Improves the Efficiency of Somatic Embryogenesis in Hevea brasiliensis

Luo,

Zhang,

Zhou

et al. 2024

IJMS

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“…Other endogenous elements, such as the availability of extender (IPP) and initiator (FPP) molecules, and suitable organelles with appropriate lipid and protein structures, such as Hevea RP, could be the reason for the variance in NR length in different species [ 2 , 4 , 13 , 21 , 32 , 55 ]. Exogenous stimuli, such as light/dark period, moisture, water, nutrient availability, temperature, etc., also greatly influence the production of secondary metabolites [ 56 , 57 , 58 ], especially NR and the length of the polymers in different species [ 4 , 59 , 60 , 61 ].…”

Section: Crispr/cas9 Mutagenesis: Cpt Alone Does Not Solely Determine...mentioning

confidence: 99%

Reviving Natural Rubber Synthesis via Native/Large Nanodiscs

Umar,

Ahmad,

2024

Polymers

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Natural rubber (NR) is utilized in more than 40,000 products, and the demand for NR is projected to reach $68.5 billion by 2026. The primary commercial source of NR is the latex of Hevea brasiliensis. NR is produced by the sequential cis-condensation of isopentenyl diphosphate (IPP) through a complex known as the rubber transferase (RTase) complex. This complex is associated with rubber particles, specialized organelles for NR synthesis. Despite numerous attempts to isolate, characterize, and study the RTase complex, definitive results have not yet been achieved. This review proposes an innovative approach to overcome this longstanding challenge. The suggested method involves isolating the RTase complex without using detergents, instead utilizing the native membrane lipids, referred to as “natural nanodiscs”, and subsequently reconstituting the complex on liposomes. Additionally, we recommend the adaptation of large nanodiscs for the incorporation and reconstitution of the RTase complex, whether it is in vitro transcribed or present within the natural nanodiscs. These techniques show promise as a viable solution to the current obstacles. Based on our experimental experience and insights from published literature, we believe these refined methodologies can significantly enhance our understanding of the RTase complex and its role in in vitro NR synthesis.

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Natural Rubber (Hevea brasiliensis [Willd. ex A. Juss.] Müll. Arg.): History, Domestication, Genetic Diversity, Conservation and Cultivar Improvement

Rao,

Meenakumari

2024

Sustainable Development and Biodiversity

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Genetics and Genomics of Abiotic Stress in Rubber Tree (Hevea Brasiliensis)

Cited by 3 publications

References 160 publications

Overexpression of HbGRF4 or HbGRF4-HbGIF1 Chimera Improves the Efficiency of Somatic Embryogenesis in Hevea brasiliensis

Overexpression of HbGRF4 or HbGRF4-HbGIF1 Chimera Improves the Efficiency of Somatic Embryogenesis in Hevea brasiliensis

Reviving Natural Rubber Synthesis via Native/Large Nanodiscs

Natural Rubber (Hevea brasiliensis [Willd. ex A. Juss.] Müll. Arg.): History, Domestication, Genetic Diversity, Conservation and Cultivar Improvement

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