Genetics, Cytology, and Evolution of Gossypium

Endrizzi, J. E.; Turcotte, E. L.; Kohel, R. J.

doi:10.1016/s0065-2660(08)60515-5

Cited by 328 publications

(338 citation statements)

References 299 publications

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“…Through manipulation of PHYA1 expression using RNAi, we have achieved simultaneous improvement of several important traits, such as fibre length and early maturity, without negatively affecting other key parameters such as yield-a goal that has largely eluded conventional breeding. For instance, introgression of superior fibre quality traits from Pima cotton (G. barbadense) into Upland (G. hirsutum) cultivars using interspecies genetic hybridization is challenging owing to segregation distortion and linkage drag that often results in introgressed progenies that are late maturing or have poor agronomic quality 30 . Application of RNAi has allowed us to effectively solve these fundamental problems, and rapidly improve maturity, flowering, yield and major fibre quality traits, thus transforming commercial Upland varieties into more long-stapled, productive and early-maturing cultivars.…”

Section: Discussionmentioning

confidence: 99%

Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L

et al. 2014

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Simultaneous improvement of fibre quality, early-flowering, early-maturity and productivity in Upland cotton (G. hirsutum) is a challenging task for conventional breeding. The influence of red/far-red light ratio on the fibre length prompted us to examine the phenotypic effects of RNA interference (RNAi) of the cotton PHYA1 gene. Here we show a suppression of up to B70% for the PHYA1 transcript, and compensatory overexpression of up to B20-fold in the remaining phytochromes in somatically regenerated PHYA1 RNAi cotton plants. Two independent transformants of three generations exhibited vigorous root and vegetative growth, early-flowering, significantly improved upper half mean fibre length and an improvement in other major fibre characteristics. Small decreases in lint traits were observed but seed cotton yield was increased an average 10-17% compared with controls. RNAi-associated phenotypes were heritable and transferable via sexual hybridization. These results should aid in the development of early-maturing and productive Upland cultivars with superior fibre quality.

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Section: Discussionmentioning

confidence: 99%

Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L

et al. 2014

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“…It has also been observed that G. raimondii (D 5 ) and G. gossypioides (D 5 ) are genetically more comparable to G. hirsutum (AD 2 ) and G. barbadense (AD 2 ) (Kebede et al 2007). In few cytogenetic studies, it was elucidated that G. herbaceum is more comparable to the ancestor species of tetraploid cotton than G. arboreum (Endrizzi et al 1985;Wendel 1989;Percival and Kohel 1990).…”

Section: Performance Of Microsatellite Between a D And Ad-genome Spementioning

confidence: 99%

Pros and cons of using genomic SSRs and EST-SSRs for resolving phylogeny of the genus Gossypium

2013

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The genus Gossypium is comprised of 50 diverse cotton species representing eight different genomes (A through G and K), however, phylogenetic relationship using various DNA marker types such as RAPD and SSRs was determined on limited number of cotton species. In this report, we have demonstrated the application of genomic SSRs (gSSRs) and EST-SSRs, and after combining both the data sets, for resolving the phylogenies of 36 cotton species including seven races. Out of the 100 primer pairs surveyed (50 for gSSRs and 50 for ESTSSRs), 75 produced scorable amplification products in all species. Out of these, 73 were found to be polymorphic and amplified 135 alleles ranging from 1 to 5 alleles per SSR marker (average 2.87 alleles per marker). The gSSRs amplified higher number of alleles (72) compared to the EST-SSRs (63). In total 22 highly informative SSRs with PIC values C0.5 were identified. Genomic SSRs containing di-while EST-SSRs containing tri-nucleotide repeats exhibited high polymorphism compared to the other nucleotide repeats containing gSSRs/EST-SSRs. Number of tandem repeats and polymorphism were positively correlated. Neither the type of chromosome nor the location of the SSRs showed association with the polymorphism. Gossypium herbaceum var. africanum (Watt) Hutch. ex and Ghose and Gossypium robinsonii F. Muell. were found the most genetically diverse, while among races of Gossypium hirsutum L. ''yucatanense'' and G. hirsutum ''punctatum'' were found genetically diverse. Of the three data sets, clustering analysis based on EST-SSRs and combined data sets, revealed parallel results reported in earlier studies. This study further confirmed that Gossypium darwinii Watt has close relationship with Gossypium barbadense L. Moreover, Gossypium raimondii Ulbr. and G. herbaceum/Gossypium arboreum L. are close living relatives of the ancestor allotetraploid species. Our studies suggest that for resolving phylogenetic relationship among the various plant species EST-SSRs could be a better choice. This information can be instrumental in transferring novel alleles or loci from the wild species into the cultivated cotton species which would set a stage for cultivating genetically diverse cultivars-a way to achieve sustainable cotton production in changing climate.

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“…The allotetraploid cotton species, which include two commercially important cultivated species, Gossypium hirsutum L. and G. barbadense L., were generated by A-and D-compound genomes (Fryxell 1992). The best living models of the ancestral A-and D-genome parents are G. herbaceum and G. raimondii, respectively (Endrizzi et al 1985). A-genome diploid cottons produce spinnable fibers and have been cultivated, while D-genome species produce very short and appressed fibers.…”

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confidence: 99%

A Microsatellite-Based, Gene-Rich Linkage Map Reveals Genome Structure, Function and Evolution in Gossypium

Guo¹,

Cai²,

et al. 2007

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The mapping of functional genes plays an important role in studies of genome structure, function, and evolution, as well as allowing gene cloning and marker-assisted selection to improve agriculturally important traits. Simple sequence repeats (SSRs) developed from expressed sequence tags (ESTs), EST-SSR (eSSR), can be employed as putative functional marker loci to easily tag corresponding functional genes. In this paper, 2218 eSSRs, 1554 from G. raimondii-derived and 754 from G. hirsutum-derived ESTs, were developed and used to screen polymorphisms to enhance our backbone genetic map in allotetraploid cotton. Of the 1554 G. raimondii-derived eSSRs, 744 eSSRs were able to successfully amplify polymorphisms between our two mapping parents, TM-1 and Hai7124, presenting a polymorphic rate of 47.9%. However, only a 23.9% (159/754) polymorphic rate was produced from G. hirsutum-derived eSSRs. No relationship was observed between the level of polymorphism, motif type, and tissue origin, but the polymorphism appeared to be correlated with repeat type. After integrating these new eSSRs, our enhanced genetic map consists of 1790 loci in 26 linkage groups and covers 3425.8 cM with an average intermarker distance of 1.91 cM. This microsatellite-based, gene-rich linkage map contains 71.96% functional marker loci, of which 87.11% are eSSR loci. There were 132 duplicated loci bridging 13 homeologous At/Dt chromosome pairs. Two reciprocal translocations after polyploidization between A2 and A3, and between A4 and A5, chromosomes were further confirmed. A functional analysis of 975 ESTs producing 1122 eSSR loci tagged in the map revealed that 60% had clear BLASTX hits (,1e À10 ) to the Uniprot database and that 475 were associated mainly with genes belonging to the three major gene ontology categories of biological process, cellular component, and molecular function; many of the ESTs were associated with two or more category functions. The results presented here will provide new insights for future investigations of functional and evolutionary genomics, especially those associated with cotton fiber improvement.

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Genetics, Cytology, and Evolution of Gossypium

Cited by 328 publications

References 299 publications

Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L

Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L

Pros and cons of using genomic SSRs and EST-SSRs for resolving phylogeny of the genus Gossypium

A Microsatellite-Based, Gene-Rich Linkage Map Reveals Genome Structure, Function and Evolution in Gossypium

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