Genetics of alginate biosynthesis in Pseudomonas aeruginosa

Ohman, Dennis E.; Goldberg, Joanna B.

doi:10.1007/978-94-009-1836-8_11

Cited by 6 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A CFTR defect leads to, among other problems, the accumulation of abnormal viscous fluids in the lungs of CF patients, which renders the patients susceptible to various bacterial infections. Pseudomonas aeruginosa in its mucoid form is a major source of infection in the CF lung (7,22,24). The mucoid morphology of the bacteria is due to the overproduction of a viscous exopolysaccharide, alginate, which is a high-molecular-weight, linear copolymer of two uronic acids, D-mannuronate and its C-5 epimer, L-guluronate (10).…”

mentioning

confidence: 99%

“…The mucoid morphology of the bacteria is due to the overproduction of a viscous exopolysaccharide, alginate, which is a high-molecular-weight, linear copolymer of two uronic acids, D-mannuronate and its C-5 epimer, L-guluronate (10). Alginate is believed to protect the bacteria from the adverse environment of the CF lung (7,22,24). Although the initial colonizing P. aeruginosa are mostly nonmucoid, they undergo conversion to a highly mucoid phenotype in later stages of the disease.…”

mentioning

confidence: 99%

“…The first gene in the cluster, algT (also called algU), is a positive regulator which promotes the mucoid phenotype and alginate overproduction. The gene algT is highly homologous to rpoE, one of the heat shock sigma factors in Escherichia coli (7,8,17,24,29). The AlgT protein has been purified, and its biochemical activity as a sigma factor has been demonstrated (15,26).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA

et al. 1996

View full text Add to dashboard Cite

Conversion from the nonmucoid to the mucoid phenotype is a typical feature of Pseudomonas aeruginosa strains causing chronic pulmonary infections in cystic fibrosis patients. One of the key genetic controls in this conversion to mucoidy is from the algT(U)-mucA-mucB(algN) locus, located at 67.5 min on the standard P. aeruginosa chromosomal map. The algT gene promotes conversion to mucoidy and encodes an alternative sigma factor ( E ) which belongs to the ECF (for extracytoplasmic function) family. On the other hand, the mucA and mucB (algN) genes suppress conversion to mucoidy. Loss-of-function mutations in mucA have been postulated to be the cause of mucoidy in some P. aeruginosa strains isolated from cystic fibrosis patients. We expressed and purified the protein products from the mucA and mucB open reading frames. The purified MucA protein abolishes the in vitro transcription specified by AlgT and the ability of AlgT to compete with an Escherichia coli sigma factor, FliA, suggesting that inhibiting AlgT-dependent transcription could be the mechanism by which mucA suppresses mucoidy in vivo. Enzyme-linked immunosorbent assay and glycerol density gradient sedimentation experiments suggest that MucA physically interacts with AlgT.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA

et al. 1996

View full text Add to dashboard Cite

show abstract

“…The mucoid phenotype is due to the influence of muc and other mutations which affect the regulation and biosynthesis of alginate in response to environmental stimuli (8,14). Much of the alginate-biosynthetic pathway has been elucidated (25), and many of the genes have been mapped onto the P. aeruginosa PAO chromosome (23,27). Also, Southern blotting and hybridization experiments have established that both mucoid and nonmucoid isolates of P. aeruginosa have chromosomal genes that encode enzymes of alginate biosynthesis.…”

mentioning

confidence: 99%

Isolation of a mucoid alginate-producing Pseudomonas aeruginosa strain from the equine guttural pouch

et al. 1992

View full text Add to dashboard Cite

The isolation and characterization of a mucoid, alginate-producing strain of Pseudomonas aeruginosa from a nonhuman host, namely, in chondroids from an equine guttural pouch, is reported for the first time. Pure cultures ofP. aeruginosa 12534 were isolated from a 17-month-old pony mare with a history of chronic bilateral mucopurulent.nasal discharge from the right guttural pouch. Transmission electron microscopy of chondroids showed mucoid P. aeruginosa growing as microcolonies within a matrix of extracellular material. On the basis of expression of the mucoid phenotype under different growth conditions, P. aeruginosa 12534 belongs to group

show abstract

Recent Developments in Bacterial Adhesion to Animal Cells

Ofek¹,

Doyle²

1994

Bacterial Adhesion to Cells and Tissues

View full text Add to dashboard Cite

Genetics of alginate biosynthesis in Pseudomonas aeruginosa

Cited by 6 publications

References 34 publications

Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA

Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA

Isolation of a mucoid alginate-producing Pseudomonas aeruginosa strain from the equine guttural pouch

Recent Developments in Bacterial Adhesion to Animal Cells

Contact Info

Product

Resources

About