Genetics of alkane oxidation byPseudomonas oleovorans

Beilen, Jan B. van; Wubbolts, Marcel; Witholt, Bernard

doi:10.1007/bf00696457

Cited by 314 publications

(246 citation statements)

References 95 publications

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“…A membrane-bound alkane hydroxylase was first discovered in a hexane-degrading fluorescent pseudomonad tentatively named Pseudomonas oleovorans [39,40], but later identified as a P. putida [41]. Biochemical studies showed that the P. putida alkane hydroxylase system consists of an integral membrane monooxygenase [42][43][44], 1 or 2 rubredoxins [45][46][47], and a rubredoxin reductase [48,49] (Fig.…”

Section: Diversity Of Alkane Hydroxylase Systemsmentioning

confidence: 99%

Diversity of Alkane Hydroxylase Systems in the Environment

Beilen

Duetz

et al. 2003

Oil & Gas Science and Technology - Rev. IFP

326

227

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Résumé -Diversité des systèmes alcane hydroxylase dans l'environnement -La première étape dans la dégradation aérobie des alcanes par les bactéries, les levures et les champignons est catalysée par des oxygénases, une classe d'enzymes capables d'introduire des atomes d'oxygène issus de l'oxygène moléculaire dans le substrat alcane. Ces enzymes jouent un rôle important dans la biodégradation du pétrole et dans la biodégradation cométabolique de composés tels que le trichloroéthylène et les éthers-carburants. De plus, ce sont des biocatalyseurs très utiles qui peuvent également servir de modèles pour caractériser une réaction chimique difficile : l'activation régio-et stéréospécifique de la liaison C-H. Plusieurs autres classes d'enzymes catalysent l'oxydation des alcanes. Les souches de levures capables de dégrader les alcanes contiennent plusieurs alcanes hydroxylases appartenant à la superfamille des P450, alors que différentes bactéries contiennent des enzymes similaires au système alcane hydroxylase membranaire de Pseudomonas putida GPo1. Les alcanes à courte chaîne sont probablement oxydés par des alcanes hydroxylases solubles similaires aux méthanes monooxygénases. La présence dans l'environnement de ces oxygénases a été étudiée dans des échantillons de sols et aquifères uniquement pour les alcanes hydroxylases associés aux membranes. Abstract -Diversity of Alkane Hydroxylase Systems in the Environment

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Section: Diversity Of Alkane Hydroxylase Systemsmentioning

confidence: 99%

Diversity of Alkane Hydroxylase Systems in the Environment

Beilen

Duetz

et al. 2003

Oil & Gas Science and Technology - Rev. IFP

326

227

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“…However, microorganisms capable of utilizing these compounds as only energy source can be found almost everywhere, even in non-contaminated sites [28,29].…”

Section: Biodegradation Of Alkanesmentioning

confidence: 99%

“…Even though several studies have shown that the alkB genes are widespread in nature [166], only the Pseudomonas putida GPo1 alkane hydroxylase system [167] has been studied in detail with respect to enzymology, genetics, as well as potential applications [28,167,168]. The alkane hydroxylase system of GPo1 consists of three components: a particulate hydroxylase (AlkB), which is the catalytic component localized in the cytoplasmic membrane, and two soluble proteins, rubredoxin (AlkG) and rubredoxin reductase (AlkT) ( Fig.…”

Section: Alkb In Pseudomonas Putida Gpo1mentioning

confidence: 99%

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Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

Cappelletti

Fedi

Honda

et al. 2010

Journal of Biotechnology

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Discussion 107 Summary 113Chapter 5 -Analysis of the alkB gene expression Summary 157Chapter 6 -Proteomic analysis of n-alkanes growth of Rhodococcus sp. BCP1 Chapter 1 -General Introduction PrefaceThe quality of life on Earth is tightly related with the overall quality of the environment [1]. During the last century it was commonly believed that the abundance of land and resources were unlimited and it was rarely acknowledged that the production, use, and disposal of hazardous substances had environmental and health effects [1][2][3]. Owing to this, human activity has deliberately or inadvertently released into waters and soil a variety of toxic compounds as refrigerants, paints, solvents, herbicides and pesticides that can cause considerable environmental pollution and human health problems as a result of their persistence, toxicity, and transformation into hazardous metabolites [4,5]. Very soon, however, it became clear how the carelessness and negligence in handling the industrial wastes and gas emissions had caused dramatic effect on the nature and on human health [1].Nowadays, the concern about the global warming and the climate changing induced the government regulatory agencies to establish procedures for assessing the environmental impact and health hazards of chemicals and for controlling/limiting their utilization.International efforts have been applied to remedy many contaminated sites all over the world, either as a response to the risk of adverse health or environmental effects caused by contamination or to enable the site to be redeveloped for use [5]. In response to public and government concern and because of the intriguing research problems presented, environmental scientists, biologists and chemists have been giving increased attention to identifying and determining the behavior and fate of organic compounds in natural ecosystems [5].Bioremediation procedures offer an economical and effective possibility to degrade or render innocuous toxic pollutants using natural biological activity [1]. By definition, bioremediation implies the use of living organisms, primarily microorganisms, to degrade the Since contaminant compounds are transformed by living organisms through reactions that take place as a part of their metabolic processes, environmental biotechnology focuses on the development of techniques to optimize environmental parameters to allow these metabolic pathways to proceed faster and, therefore, to improve the bio-degradation [1]. As a result, the removal of the contaminated soil can be necessary to let the biodegradation proceed in controlled sites (such as bioreactors). These ex situ approaches provide optimal conditions out one single step of the entire bio-degradation process. Hydrocarbons in the environmentHydrocarbons are energy-rich organic compounds consisting of carbon and hydrogen atoms and they can be saturated (only single C-H bounds) or unsaturated (one ore more double or triple C-H bounds). Alkanes are saturated hydrocarbons with the general formula C n H 2n+2 ; they can b...

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“…Many bacterial species play an active role in the process of petroleum fuel biodegradation. Some biodegradable hydrocarbons such as n-alkanes are known to be utilised by a large number of microorganisms as a sole source of carbon and energy 29,[53][54][55] . Polycyclic aromatic hydrocarbons and cyclo-alkanes are mainly degraded by co-oxidation in the environment 56 .…”

Section: Bioremediation: Why ? and How ?mentioning

confidence: 99%

Microbial Bioremediation of Fuel Oil Hydrocarbons in Marine Environment

Pavitran

Jagtap

Subramanian

et al. 2006

DSJ

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Pollution in marine environment due to heavier petroleum products such as high-speed diesel is known to take from days to months for complete natural remediation owing to its low volatility. For the survival of marine flora and fauna, it is important to control pollution caused by such recalcitrant and xenobiotic substances. Several petroleum hydrocarbons found in nature are toxic and recalcitrant. Therefore, pollution due to high-speed diesel is a cause of concern. The natural dispersion of high-speed diesel, a slow process, is attributed to an overall combined effect of physico-chemical and biological processes which take months for complete dispersion. History of marine oil spill bioremediation indicates limited laboratory studies. But experiences from various oil spill management and field trials indicate important role of bioremediation, where, biodegradation of hydrocarbons through microbial mediators plays a major role in pollutant oil dispersion. These microbial mediators such as bioemulsifiers and fimbrae, help in emulsification, dispersion, allowing attachment of bacteria to oil layers, followed by substrate-specific enzymatic biodegradation in water.

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Genetics of alkane oxidation byPseudomonas oleovorans

Cited by 314 publications

References 95 publications

Diversity of Alkane Hydroxylase Systems in the Environment

Diversity of Alkane Hydroxylase Systems in the Environment

Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

Microbial Bioremediation of Fuel Oil Hydrocarbons in Marine Environment

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