Genetics of Bacteria: Role in Pathogenesis of Infection of the Respiratory Tract

Klingman, Karin L.; Murphy, Timothy F.

doi:10.1007/978-3-0348-8831-8_9

Cited by 1 publication

(1 citation statement)

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“…Secretory leukoprotease inhibitor is an antibacterial protein secreted by cells at mucosal surfaces. It binds tightly to mucins, protecting them against proteolysis by neutrophil elastase 35. It has been demonstrated36 that mucin gene expression, induced by cystic fibrosis respiratory stimuli, can be inhibited by SLP1 , indicating its potential importance as an antimucin agent.…”

Section: Commentmentioning

confidence: 99%

Gene Expression Differences in Infected and Noninfected Middle Ear Complementary DNA Libraries

Kerschner

Horsey²,

Ahmed³

et al. 2009

Arch Otolaryngol Head Neck Surg

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Objectives To investigate genetic differences in middle ear mucosa (MEM) with nontypeable Haemophilus influenzae (NTHi) infection. Genetic upregulation and downregulation occurs in MEM during otitis media (OM) pathogenesis. A comprehensive assessment of these genetic differences using the techniques of complementary DNA (cDNA) library creation has not been performed. Design The cDNA libraries were constructed from NTHi-infected and noninfected chinchilla MEM. Random clones were picked, sequenced bidirectionally, and submitted to the National Center for Biotechnology Information (NCBI) Expressed Sequence Tags database, where they were assigned accession numbers. These numbers were used with the basic local alignment search tool (BLAST) to align clones against the nonredundant nucleotide database at NCBI. Results Analysis with the Web-based statistical program FatiGO identified several biological processes with significant differences in numbers of represented genes. Processes involved in immune, stress, and wound responses were more prevalent in the NTHi-infected library. S100 calcium-binding protein A9 (S100A9); secretory leukoprotease inhibitor (SLPI); β2-microglobulin (B2M); ferritin, heavy-chain polypeptide 1 (FTH1); and S100 calcium-binding protein A8 (S100A8) were expressed at significantly higher levels in the NTHi-infected library. Calcium-binding proteins S100A9 and S100A8 serve as markers for inflammation and have antibacterial effects. Secretory leukoprotease inhibitor is an antibacterial protein that inhibits stimuli-induced MUC1, MUC2, and MUC5AC production. Conclusions A number of genes demonstrate changes during the pathogenesis of OM, including SLPI, which has an impact on mucin gene expression; this expression is known to be an important regulator in OM. The techniques described herein provide a framework for future investigations to more thoroughly understand molecular changes in the middle ear, which will likely be important in developing new therapeutic and intervention strategies.

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Section: Commentmentioning

confidence: 99%