Genetics of Clostridium

Young, Michael; Staudenbauer, Walter L.; Minton, Nigel P.

doi:10.1007/978-1-4757-9718-3_3

Cited by 24 publications

(19 citation statements)

References 136 publications

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“…The GC content of the DNA was approximately 28%, which is in the range characteristic of genes from C. pasteurianum (36). The codon usage also showed a strong bias toward the use of A or U in the wobble position, again a characteristic feature of clostridiae (36). The two amino acid sequence motifs (YAIACCV and TIRVSGY) are completely conserved between the E. coli and C. pasteurianum enzymes (Fig.…”

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confidence: 83%

“…These data suggest that the pfl gene does not form part of an operon and that it is monocistronic. The GC content of the DNA was approximately 28%, which is in the range characteristic of genes from C. pasteurianum (36). The codon usage also showed a strong bias toward the use of A or U in the wobble position, again a characteristic feature of clostridiae (36).…”

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confidence: 87%

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Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum

Weidner¹,

Sawers²

1996

J Bacteriol

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Formate is the major source of C1 units in many species of the genus Clostridium. In this study we have cloned and characterized the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum. The genetic and transcriptional organizations of the genes and the high level of homology exhibited by the respective gene products to their Escherichia coli counterparts indicate strong evolutionary conservation of these enzymes.

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confidence: 83%

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confidence: 87%

Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum

Weidner¹,

Sawers²

1996

J Bacteriol

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“…Interest in the physiologically diverse genus Clostnidium has been renewed because of the presence of species with recognized industrial potential (1,2,15,31,32) as well as anaerobic pathogenic properties (25). There currently is considerable industrial interest in the nontoxinogenic clostridia as practical biomass conversion reactors for production of solvents and organic acids (1,2,15).…”

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confidence: 99%

“…To understand clostridial metabolic pathways related to the production of industrial chemicals, (i.e., Clostridium acetobutylicum ABE fermentation) or anaerobic pathogenesis, (i.e., C. perfringens intoxication), systems for genetic analysis (e.g., transformation and stable expression vectors) need to be developed. C. acetobutylicum and C. perfringens represent the model species for clostridial genetics (31,32).…”

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Construction and characterization of a phage-plasmid hybrid (phagemid), pCAK1, containing the replicative form of viruslike particle CAK1 isolated from Clostridium acetobutylicum NCIB 6444

Kim

Blaschek

1993

J Bacteriol

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A bacteriophage-plasmid hybrid (phagemid) designated pCAK1 was constructed by ligating 5-kbp Escherichia coli plasmid pAK102 (Apr Emr) and the 6.6-kbp HaeIII-linearized replicative form of the CAKi viruslike particle from Clostridium acetobutylicum NCIB 6444. Phagemid pCAK1 (11.6 kbp) replicated via the ColEl replication origin derived from pAK102 in E. coli. Single-stranded DNA (ssDNA) molecules complexed with protein in a manner which protected ssDNA from nucleases were recovered from the supernatant of E. coli DH11S transformants containing pCAK1 in the absence of cell lysis. This suggests that the viral-strand DNA synthesis replication origin of CAKi and associated gene expression are functional in E. coli DH11S. The single-stranded form of pCAK1 isolated from E. coli supernatant was transformed into E. coli DH5ct' or DH11S by electroporation. Isolation of ampicillin-resistant E. coli transformants following transformation suggests that the complementary-strand DNA synthesis replication origin of CAKi is also functional in E. coli.The coat proteins associated with ssDNA of pCAK1 demonstrated sensitivity to proteinase K and various solvents (i.e., phenol and chloroform), similar to the results obtained previously with CAK1. Following phagemid construction in E. coli, pCAK1 was transformed into C. acetobutylicum ATCC 824 and C.perfringens 13 by intact cell electroporation. Restriction enzyme analysis of pCAK1 isolated from erythromy.cin-resistant transformants of both C. acetobutylicum and C. pefringens suggested that it was identical to that present in E. coli transformants.

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Anaerobes, Industrial Uses

Jain¹,

Zeikus²

1999

Encyclopedia of Bioprocess Technology

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Genetics of Clostridium

Cited by 24 publications

References 136 publications

Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum

Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum

Construction and characterization of a phage-plasmid hybrid (phagemid), pCAK1, containing the replicative form of viruslike particle CAK1 isolated from Clostridium acetobutylicum NCIB 6444

Anaerobes, Industrial Uses

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