Genetics of Plant Isozymes

Weeden, N. F.; Wendel, Jonathan F.

doi:10.1007/978-94-009-1840-5_3

Cited by 462 publications

(263 citation statements)

References 192 publications

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“…Glucose-6-phosphate isomerase, GOT, and TPI were assayed on a lithium borate buffer at pH 8.3 (Soltis et al 1983). Loci banding patterns were consistent with typical subunit structures (Weeden & Wendel 1989). Polymorphic loci conformed to Mendelian expectations for segregation in the analysed progeny arrays.…”

Section: Methodsmentioning

confidence: 97%

The post-fire effects on the outcrossing rate of a Brazilian savannah shrub, Helicteres sacarolha A.St.-Hil

Franceschinelli

Bawa

2005

Rev. bras. Bot.

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-(The post-fire effects on the outcrossing rate of a Brazilian savannah shrub, Helicteres sacarolha A.St.-Hil.). The effect of post-fire, plant density and number of flowers per plant on outcrossing rates was examined in a Brazilian savannah shrub, Helicteres sacarolha. Data on number of flowering plants and flowers per plant were collected during the flowering season in January and February of 1994. In October of 1994, a fire swept through the studied area after 30 years of fire absence. The burnt plants of H. sacarolha resprouted, producing flowers and fruits in 1995. Seeds from several plants in both years were collected. Allozyme markers were used to estimate the multilocus outcrossing rates for 1994 and 1995 progenies. After the fire, outcrossing rates increased. In 1995, plants flowered vigorously, increasing flower density and probably pollinator activity. Helicteres sacarolha seems to be fire resistant, like other plant species of the Brazilian savannah, but several plants tagged in 1994 were not found after the fire, and may have died. Thus, although genetic diversity and outcrossing rates increased following the fire in 1994, repeated events of fire may decrease drastically the population size of H. sacarolha, leading to a diminished genetic diversity and outcrossing rates.Key words -cerrado, flower density, Helicteres, plant outcrossing rate, post-fire effect RESUMO -(Os efeitos pós-fogo na taxa de cruzamento de um arbusto do cerrado, Helicteres sacarolha A.St.-Hil.). Os efeitos do fogo, da densidade de plantas, do número de flores por planta na taxa de cruzamento foram checados para uma espécie de arbusto do cerrado, Helicteres sacarolha. Dados de número de plantas e flores por planta foram coletados durante o período de floração de janeiro a fevereiro de 1994. Em outubro de 1994, uma queimada ocorreu na área de estudo após 30 anos de ausência de fogo. No entanto, as plantas queimadas de H. sacarolha brotaram, produzindo flores e frutos em 1995. Sementes de várias plantas foram coletadas em 1994 e 1995. Marcadores aloenzimáticos foram utilizados para estimar a taxa de cruzamento para as progênies de 1994 e 1995. A taxa de cruzamento aumentou após a ocorrência do fogo. Em 1995, as plantas floresceram vigorosamente, aumentando a densidade de flores e, provavelmente, a atividade dos polinizadores entre as plantas da população. Como muitas espécies do cerrado brasileiro, H. sacarolha é tolerante ao fogo, contudo várias plantas marcadas em 1994 não foram encontradas após o fogo e podem ter morrido queimadas. Desta forma, eventos repetidos de fogo podem levar a uma drástica diminuição do tamanho da população de H. sacarolha e, conseqüentemente, a um decréscimo em suas taxas de diversidade genética e de cruzamento.Palavras-chave -cerrado, densidade de flor, efeito do fogo, Helicteres, taxa de cruzamento

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Section: Methodsmentioning

confidence: 97%

The post-fire effects on the outcrossing rate of a Brazilian savannah shrub, Helicteres sacarolha A.St.-Hil

Franceschinelli

Bawa

2005

Rev. bras. Bot.

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“…Different methods have been used to evaluate the level of genetic distance, including morphological markers, isozymes and molecular techniques (Weeden and Wendel 1989;Ferriol et al 2003;Smýkal et al 2008). Significant time and effort are required to obtain morphological markers, which are frequently influenced by environmental factors.…”

Section: Introductionmentioning

confidence: 99%

Combining ability, heterosis, genetic distance and their intercorrelations for waterlogging tolerance traits in chrysanthemum

Zhang

Yang

et al. 2017

Euphytica

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Waterlogging tolerance (WT) is a major objective in chrysanthemum breeding programs, and although certain genotypes with different tolerance levels have been identified, their value as parents for WT breeding is unknown. Here, twelve F 1 crosses derived from an incomplete diallel mating scheme were conducted to investigate combining ability and heterosis for WT and their relationships with parental genetic distance. The results showed that the membership function value of waterlogging (MFVW) was controlled by additive and non-additive gene effects, whereas other growth and biomass traits were mainly controlled by non-additive gene effects. The estimated broad and narrow sense heritabilities of the MFVW were 97.5 and 51.5%, respectively. Combining ability analyses indicated that 'Nannong Xuefeng' showing the largest general combining ability (GCA) effect for the MFVW was the best combiner, and identified several best cross combinations with high positive specific combining ability (SCA) effects for most WT-related traits. Mid-and high-parent heterosis occurred widely. The three distance measures, based on phenotypic traits (PD), molecular markers (GD) and markers linked with quantitative trait loci (QTL-GD), presented a nonsignificant correlation with combining ability except for the GD with GCA for the relative root fresh weight. The correlations between the QTL-GD and heterosis were significant for certain traits and generally higher than that of the PD or GD and heterosis. The SCA effects were positively correlated with heterosis for most of the WT traits (0.51 B r B 0.80). The findings benefit understanding the inheritance mode and then achieving desirable improvement for WT in chrysanthemum.

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“…For gels staining, protocols of Scandalios (1964) were used for α and β-Est. ; Wendel & Weeden (1989) for both Ao and Acp; Weeden & Wendel (1990) for Adh; Jonathan & Wendell (1990) for Mdh & Heldt (1997) for Px. After run finished, gels were washed two or three times with tap water; fixed in ethanol: 20% glacial acetic acid (9:11 v/v) for 24 hr and photographed.…”

Section: Methodsmentioning

confidence: 99%

Preliminary Authentication of Some Acacia L. Species in Taif Highlands

2015

Egypt. J. Bot.

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LTHOUGH their wide distribution in arid lands and their many uses that include fodder and fuel besides the environmental values of soil stabilization, Acacia species are vulnerable to elimination in Saudi Arabia. In this study, seed morphology and patterns of their coat surface sculpture as revealed by scanning electron microscopy besides both seed proteins and seven isozymes profiles were employed for the discrimination and authentication the vulnerable Saudi Arabian Acacia collected from the western region of the kingdom. The scanning electron microscopic study displayed diversity in shape, dimensions, color, central aerole features and coat topography of seeds among different species to be characteristic for each species. Seed protein and isozyme profiles showed high variability among studied species. The UPGMA phenogram and genetic similarity analysis based on combination of seed morphology, protein and isozyme patterns confirmed the extensive genetic diversity existed in Acacia species.

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Genetics of Plant Isozymes

Cited by 462 publications

References 192 publications

The post-fire effects on the outcrossing rate of a Brazilian savannah shrub, Helicteres sacarolha A.St.-Hil

The post-fire effects on the outcrossing rate of a Brazilian savannah shrub, Helicteres sacarolha A.St.-Hil

Combining ability, heterosis, genetic distance and their intercorrelations for waterlogging tolerance traits in chrysanthemum

Preliminary Authentication of Some Acacia L. Species in Taif Highlands

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