Genetics of Resistance to Wheat Leaf Rust

Kolmer, J. A.

doi:10.1146/annurev.phyto.34.1.435

Cited by 355 publications

(258 citation statements)

References 103 publications

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“…Another cultivar, Cappelle Desprez, was postulated to have Lr34 based on the observed genetic association of leaf and stripe rust resistance (McIntosh 1992 The Canadian cultivar, AC Domain was postulated to contain Lr34 (Kolmer 1996) and also carries the Lr34-linked allele at the csLV34 locus (Kolmer et al 2008). Two wheat cultivars, Jagger and H45, classified as susceptible to leaf rust and stripe rust respectively on adult plants were shown to have Lr34-associated alleles of the csLV34 marker (Kolmer et al 2008).…”

Section: Previous Tests With the Tightly Linkedmentioning

confidence: 99%

Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens

et al. 2009

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The locus Lr34/Yr18/Pm38 confers partial and durable resistance against the devastating fungal pathogens leaf rust, stripe rust, and powdery mildew. In previous studies, this broad-spectrum resistance was shown to be controlled by a single gene which encodes a putative ATP-binding cassette transporter. Alleles of resistant and susceptible cultivars differed by only three sequence polymorphisms and the same resistance haplotype was found in the three independent breeding lineages of Lr34/Yr18/Pm38. Hence, we used these conserved sequence polymorphisms as templates to develop diagnostic molecular markers that will assist selection for durable multi-pathogen resistance in breeding programs. Five allele-specific markers (cssfr1-cssfr5) were developed based on a 3 bp deletion in exon 11 of the Lr34-gene, and one marker (cssfr6) was derived from a single nucleotide polymorphism in exon 12. Validation of reference genotypes, well characterized for the presence or absence of the Lr34/Yr18/Pm38 resistance locus, demonstrated perfect diagnostic values for the newly developed markers. By testing the new markers on a larger set of wheat cultivars, a third Lr34 haplotype, not described so far, was discovered in some European winter wheat and spelt material. Some cultivars with uncertain Lr34 status were re-assessed using the newly derived markers. Unambiguous identification of the Lr34 gene aided by the new markers has revealed that some wheat cultivars incorrectly postulated as having Lr34 may possess as yet uncharacterised loci for adult plant leaf and stripe rust resistance. AbstractThe locus Lr34/Yr18/Pm38 confers partial and durable resistance against the devastating fungal pathogens leaf rust, stripe rust, and powdery mildew. In previous studies, this broad-spectrum resistance was shown to be controlled by a single gene which encodes a putative ATP-binding cassette transporter. Alleles of resistant and susceptible cultivars differed by only three sequence polymorphisms and the same resistance haplotype was found in the three independent breeding lineages of Lr34/Yr18/Pm38. Hence, we used these conserved sequence polymorphisms as templates to develop diagnostic molecular markers that will assist selection for durable multi-pathogen resistance in breeding programs. Five allele-specific markers (cssfr1 -cssfr5) were developed based on a 3 bp deletion in exon 11 of the Lr34-gene, and one marker (cssfr6) was derived from a SNP in exon 12. Validation of reference genotypes, well characterized for the presence or absence of the Lr34/Yr18/Pm38 resistance locus, demonstrated perfect diagnostic values for the newly developed markers. By testing the new markers on a larger set of wheat cultivars, a third Lr34 haplotype, not described so far, was discovered in some European winter wheat and spelt material. Some cultivars with uncertain Lr34 status were re-assessed using the newly derived markers. Unambiguous identification of the Lr34 gene aided by the new markers has revealed that some wheat cultivars incorrectly postula...

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Section: Previous Tests With the Tightly Linkedmentioning

confidence: 99%

Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens

et al. 2009

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“…Both genes are typical NBS-LRR type resistance genes. Host genetic resistance to leaf rust can be utilized eVectively through proper knowledge of the identity of resistance genes in commonly used wheat cultivars (Kolmer 1996). A number of leaf rust resistance genes have been transferred from wild relatives to the cultivated forms of wheat with the objective of improving the resistance of existing wheat cultivars.…”

Section: Introductionmentioning

confidence: 99%

Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers

et al. 2007

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Leaf rust caused by the fungus Puccinia triticina is one of the most important diseases of wheat (Triticum aestivum) worldwide. The use of resistant wheat cultivars is considered the most economical and environment-friendly approach in controlling the disease. The Lr38 gene, introgressed from Agropyron intermedium, confers a stable seedling and adult plant resistance against multiple isolates tested in Europe. In the present study, 94 F 2 plants resulting from a cross made between the resistant Thatcherderived near-isogenic line (NIL) RL6097, and the susceptible Ethiopian wheat cultivar Kubsa were used to map the Thatcher Lr38 locus in wheat using simple sequence repeat (SSR) markers. Out of 54 markers tested, 15 SSRs were polymorphic between the two parents and subsequently genotyped in the population. The P. triticina isolate DZ7-24 (race FGJTJ), discriminating Lr38 resistant and susceptible plants, was used to inoculate seedlings of the two parents and the segregating population. The SSR markers Xwmc773 and Xbarc273 Xanked the Lr38 locus at a distance of 6.1 and 7.9 cM, respectively, to the proximal end of wheat chromosome arm 6DL. The SSR markers Xcfd5 and Xcfd60 both Xanked the locus at a distance of 22.1 cM to the distal end of 6DL. In future, these SSR markers can be used by wheat breeders and pathologists for marker assisted selection (MAS) of Lr38-mediated leaf rust resistance in wheat.

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“…The main researches in the direction of age resistance of wheat to diseases had been carried out in the USA 10 . Later researches established that that some resistance genes to leaf rust, was effective in Europe, USA , Canada, were not effective in other countries 11 .…”

Section: Resultsmentioning

confidence: 99%

Specific Resistance Hybrids of Hexaploid Synthetic Wheat (HSW) and Commercial Cultivars to Stripe Rust in Kazakhstan

Suleimanova¹,

Dutbayev²,

Sultanova³

2016

Biosci., Biotech. Res. Asia

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Studying of inheritance of resistance to stripe rust in 2015 in F1 generation of crosses in crosses of lines of synthetic wheat with commercial cultivars of winter have been conducted on adult plants and on seedlings. On natural background of spores of Puccinia striiformis West 79% hybrids showed high resistance on adult plants. On artificial background the evaluation of seedlings showed that 46% of lines showed resistance to this disease. As the result, adult plants and seedlings wheat hybrids have showed different resistance to stripe rust. The studying of correlation dependence between spikes productivity components in artificial and in natural background of spores of P. striiformis West. pointed that during first case on seedlings have been pointed previously moderate and high correlation between indexes of spikes productivity in comparative with case on adult plants with natural inoculations which showed low correlation.

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Genetics of Resistance to Wheat Leaf Rust

Cited by 355 publications

References 103 publications

Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens

Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens

Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers

Specific Resistance Hybrids of Hexaploid Synthetic Wheat (HSW) and Commercial Cultivars to Stripe Rust in Kazakhstan

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