Genetics of Thermus

Raven, Neil

doi:10.1007/978-1-4615-1831-0_6

Cited by 7 publications

(2 citation statements)

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“…Shuttle vectors with both mesophilic and thermophilic replication functions have been constructed, and various thermophilic selection markers (Km R , trpB, leuB and b-gal) have been used (Raven 1995). Expression of cloned genes on plasmid (Koyama et al 1990;Mather and Fee 1992;Lee et al 1994;Maseda and Hoshino 1995;Wayne and Xu 1997) and integrative (Weber et al 1995;Tamakoshi et al 1997) vectors in Thermus has also been achieved.…”

Section: Introductionmentioning

confidence: 99%

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Inducible and constitutive expression using new plasmid and integrative expression vectors for Thermus sp.

Kayser

Kwak

Park

et al. 2001

Lett Appl Microbiol

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Aims: To develop molecular tools and examine inducible and constitutive gene expression in Thermus thermophilus. Methods and Results: Two plasmid promoter probe vectors and an integrative promoter probe vector were constructed using a promoterless thermostable kanamycin nucleotidyltransferase (Km R ) cassette. Three expression vectors were constructed based on a constitutive promoter J17, that functions in both Thermus and Escherichia coli. An inducible expression vector was constructed using the heat-shock inducible promoter (70 to 85°C) from the dnaK gene of T.¯avus, and the malate dehydrogenase gene (mdh) from T.¯avus was cloned and expressed in both E. coli and T. thermophilus HB27. Conclusions: This report describes the construction and use of improved promoter probe and expression vectors for use in Thermus species. The mdh gene can be used as a high temperature (85°C) reporter gene for Thermus sp. The dnaK promoter is thermo-inducible. Signi®cance and Impact of the Study: The expression vectors and molecular tools described here are signi®cant improvements over previously reported vectors for Thermus sp. The mdh gene and the thermo-inducible dnaK promoter will facilitate high temperature studies employing Thermus species.

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Section: Introductionmentioning

confidence: 99%

“…Shuttle vectors with both mesophilic and thermophilic replication functions have been constructed, and various thermophilic selection markers (Km R , trpB , leuB and β‐ gal ) have been used (Raven 1995). Expression of cloned genes on plasmid (Koyama et al .…”

Section: Introductionmentioning

confidence: 99%

Inducible and constitutive expression using new plasmid and integrative expression vectors for Thermus sp.

Kayser

Kwak

Park

et al. 2001

Lett Appl Microbiol

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Thermus

Costa

Nobre

Rainey

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Ther' mus . Gr. adj. thermos hot; M.L. masc. n. Thermus to indicate an organism living in hot places. “Deinococcus‐Thermus” / Deinococci / Thermales / Thermaceae / Thermus Straight rods , 0.5–0.8 µm in diameter; the cell length is variable. Short filaments are also formed under some culture conditions. Some strains have a stable filamentous morphology. Nonmotile ; do not possess flagella. Endospores are not observed. Stain Gram‐negative. Most strains form yellow‐pigmented colonies , some strains are nonpigmented. Aerobic with a strictly respiratory type of metabolism, but some strains grow anaerobically with nitrate and nitrite as terminal electron acceptors. Oxidase positive and catalase positive. Thermophilic , with an optimum growth temperature of about 70–75°C; most strains have a maximum growth temperature below 80°C, but some strains grow at higher temperatures. The optimum pH is about 7.8. Menaquinone 8 is the predominant respiratory quinone; ornithine is the principal diamino acid of the peptidoglycan. One major phospholipid and one major glycolipid dominate the polar lipid pattern on thin‐layer chromatography. Additional phospholipids and glycolipids are minor components. Fatty acids are predominantly iso‐ and anteiso‐branched ; branched chain 3‐hydroxy fatty acids are present in some strains. Proteins and peptides are hydrolyzed by all strains. Starch is hydrolyzed by some strains. Monosaccharides, disaccharides, amino acids, and organic acids are used as sole carbon and energy sources. The utilization of pentoses and polyols is very rare. Most strains require yeast extract or cofactors for growth. Found in hydrothermal areas with neutral to alkaline pH, also commonly isolated from man‐made thermal environments. The mol % G + C of the DNA is : 57–65. Type species : Thermus aquaticus Brock and Freeze 1969, 295.

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Isolation and characterization of Thermus bacteriophages

Slater

Ackermann

2005

Arch Virol

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One-hundred-fifteen bacteriophage strains were isolated from alkaline hot springs in Iceland, New Zealand, Russia (Kamchatka), and the U.S.A. The phages belonged to the Myoviridae, Siphoviridae, Tectiviridae, and Inoviridae families. Over 50% of isolates were isometric or filamentous. One type of siphovirus had giant tails of over 800 nm in length. Phages were further characterized by host range, genome size, DNA restriction endonuclease digestion patterns, and temperature and pH sensitivity. Myoviruses and tectiviruses had a worldwide distribution. Most phages were narrowly host-specific and all were highly resistant against heating and alkaline and acidic pH. This is the first time that tectiviruses and filamentous phages are reported for bacteria of the Thermus-Deinococcus phylum. The presence of tectiviruses, inoviruses, and myoviruses is attributed to acquisition from ancestral gamma-proteobacteria by horizontal gene transfer.

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Genetics of Thermus

Cited by 7 publications

References 49 publications

Inducible and constitutive expression using new plasmid and integrative expression vectors for Thermus sp.

Inducible and constitutive expression using new plasmid and integrative expression vectors for Thermus sp.

Thermus

Isolation and characterization of Thermus bacteriophages

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