The paper focuses on teaching assistants and their profi le in the context of personality traits. The aim of the study is to describe and examine the distribution of personality traits among teaching assistants in the Olomouc Region. Their personality traits were examined using the NEO Personality Inventory. The inventory was part of an online battery which was sent by email to all elementary schools in the Olomouc Region. We obtained a research sample of 159 respondents. The result is a description of personality traits among teaching assistants in the Olomouc Region. Compared with the standard, a statistically signifi cant diff erence was confi rmed in the dimensions of openness to experience, neuroticism, conscientiousness and agreeableness. In terms of extraversion, no statistically signifi cant diff erence was observed. In the conclusion section, the results are put into context with other professions.