Gengnianchun Extends the Lifespan of <i>Caenorhabditis elegans</i> via the Insulin/IGF‐1 Signalling Pathway

Meng, Fanhui; Li, Jun; Rao, Yanqiu; Wang, Wenjun; Fu, Yan

doi:10.1155/2018/4740739

Cited by 25 publications

(20 citation statements)

References 31 publications

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“…Insulin/IGF-1-like signaling is considered to be the first pathway to regulate lifespan of C. elegans (Meng & Li, 2018). In addition, it plays important roles in stress resistance, metabolism, and reproduction (Lee et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Didymin improves UV irradiation resistance in C. elegans

Zhou

Wang

Zhang

et al. 2019

PeerJ

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Didymin, a type of flavono-o-glycoside compound naturally present in citrus fruits, has been reported to be an effective anticancer agent. However, its effects on stress resistance are unclear. In this study, we treated Caenorhabditis elegans with didymin at several concentrations. We found that didymin reduced the effects of UV stressor on nematodes by decreasing reactive oxygen species levels and increasing superoxide dismutase (SOD) activity. Furthermore, we found that specific didymin-treated mutant nematodes daf-16(mu86) & daf-2(e1370), daf-16(mu86), akt-1(ok525), akt-2(ok393), and age-1(hx546) were susceptible to UV irradiation, whereas daf-2(e1371) was resistant to UV irradiation. In addition, we found that didymin not only promoted DAF-16 to transfer from cytoplasm to nucleus, but also increased both protein and mRNA expression levels of SOD-3 and HSP-16.2 after UV irradiation. Our results show that didymin affects UV irradiation resistance and it may act on daf-2 to regulate downstream genes through the insulin/IGF-1-like signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Didymin improves UV irradiation resistance in C. elegans

Zhou

Wang

Zhang

et al. 2019

PeerJ

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“…Many plants or plant derivatives such as green tea [31], tomatidine [32], Streblus asper [33], Paullinia cupana [34], Gengnianchun [35], and mulberry [36] were observed to extend lifespan and healthspan along with improving stress response and antioxidant mechanism in C. elegans. The present study tries to understand the effect of C. nervosum in extending lifespan and healthspan, incorporating neuroprotection along with improving stress resistance in C. elegans .…”

Section: Introductionmentioning

confidence: 99%

Antiaging, Stress Resistance, and Neuroprotective Efficacies ofCleistocalyx nervosumvar.panialaFruit Extracts UsingCaenorhabditis elegansModel

Prasanth

Brimson

Chuchawankul

et al. 2019

Oxidative Medicine and Cellular Longevity

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Plant parts and their bioactive compounds are widely used by mankind for their health benefits. Cleistocalyx nervosum var. paniala is one berry fruit, native to Thailand, known to exhibit various health benefits in vitro. The present study was focused on analyzing the antiaging, stress resistance, and neuroprotective effects of C. nervosum in model system Caenorhabditis elegans using physiological assays, fluorescent imaging, and qPCR analysis. The results suggest that the fruit extract was able to significantly extend the median and maximum lifespan of the nematode. It could also extend the healthspan by reducing the accumulation of the “age pigment” lipofuscin, inside the nematode along with regulating the expression of col-19, egl-8, egl-30, dgk-1, and goa-1 genes. Further, the extracts upregulated the expression of daf-16 while downregulating the expression of daf-2 and age-1 in wild-type nematodes. Interestingly, it could extend the lifespan in DAF-16 mutants suggesting that the extension of lifespan and healthspan was dependent and independent of DAF-16-mediated pathway. The fruit extract was also observed to reduce the level of Reactive Oxygen Species (ROS) inside the nematode during oxidative stress. The qPCR analysis suggests the involvement of skn-1 and sir-2.1 in initiating stress resistance by activating the antioxidant mechanism. Additionally, the fruit could also elicit neuroprotection as it could extend the median and maximum lifespan of transgenic strain integrated with Aβ. SKN-1 could play a pivotal role in establishing the antiaging, stress resistance, and neuroprotective effect of C. nervosum. Overall, C. nervosum can be used as a nutraceutical in the food industry which could offer potential health benefits.

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“… R04A9.7 3565956 The traditional Chinese medicine Gengnianchun (GNC) prolongs the lifespan of C. elegans . Comparison of genome-wide transcriptional profiling of untreated and GNC treated worms at day 22 showed that GNC downregulated the expression of R04A9.7 [ 60 ]. Gene symbol NCBI gene ID Ageing relevance evidence rbx-1 179358 RNAi of rbx-1 results terminal stage arrest during embryogenesis[ 61 ].…”

Section: Resultsmentioning

confidence: 99%

3D Network exploration and visualisation for lifespan data

et al. 2018

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BackgroundThe Ageing Factor Database AgeFactDB contains a large number of lifespan observations for ageing-related factors like genes, chemical compounds, and other factors such as dietary restriction in different organisms. These data provide quantitative information on the effect of ageing factors from genetic interventions or manipulations of lifespan. Analysis strategies beyond common static database queries are highly desirable for the inspection of complex relationships between AgeFactDB data sets. 3D visualisation can be extremely valuable for advanced data exploration.ResultsDifferent types of networks and visualisation strategies are proposed, ranging from basic networks of individual ageing factors for a single species to complex multi-species networks. The augmentation of lifespan observation networks by annotation nodes, like gene ontology terms, is shown to facilitate and speed up data analysis. We developed a new Javascript 3D network viewer JANet that provides the proposed visualisation strategies and has a customised interface for AgeFactDB data. It enables the analysis of gene lists in combination with AgeFactDB data and the interactive visualisation of the results.ConclusionInteractive 3D network visualisation allows to supplement complex database queries by a visually guided exploration process. The JANet interface allows gaining deeper insights into lifespan data patterns not accessible by common database queries alone. These concepts can be utilised in many other research fields.Electronic supplementary materialThe online version of this article (10.1186/s12859-018-2393-x) contains supplementary material, which is available to authorized users.

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Gengnianchun Extends the Lifespan of Caenorhabditis elegans via the Insulin/IGF‐1 Signalling Pathway

Cited by 25 publications

References 31 publications

Didymin improves UV irradiation resistance in C. elegans

Didymin improves UV irradiation resistance in C. elegans

Antiaging, Stress Resistance, and Neuroprotective Efficacies ofCleistocalyx nervosumvar.panialaFruit Extracts UsingCaenorhabditis elegansModel

3D Network exploration and visualisation for lifespan data

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