Genistein ameliorates cardiac inflammation and oxidative stress in streptozotocin-induced diabetic cardiomyopathy in rats

Gupta, Suresh; Dongare, Shirish; Mathur, Rajani; Mohanty, Ipseeta Ray; Srivastava, Sushma; Mathur, Sandeep; Nag, Tapas Chandra

doi:10.1007/s11010-015-2483-2

Cited by 94 publications

(56 citation statements)

References 58 publications

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“…Gen exerts the therapeutic function in cardiovascular diseases through various mechanisms, including estrogenic activity [30], antioxidant [31], metabolism regulation [32], and so on. Recently, Gen is identified to be an important biological cytoprotective agent, due to its capability of preventing myocardial ischemic damage [32].…”

Section: Discussionmentioning

confidence: 99%

Genistein Protects H9c2 Cardiomyocytes against Chemical Hypoxia-Induced Injury via Inhibition of Apoptosis

Shi¹,

Zhang²,

Hu³

et al. 2019

Pharmacology

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Background/Aims: Hypoxia can induce cell injury in cardiomyocytes and further lead to cardiovascular diseases. Genistein (Gen), the predominant isoflavone found in soy products, has shown protective effects on cardiovascular system. The aim of the present study was to investigate the cardioprotective effect of Gen against chemical hypoxia-induced injury. Methods: Cobalt chloride (CoCl₂) was administrated to trigger chemical hypoxia in H9c2 cardiomyocytes. Cell proliferation was detected by using MTT assay. The expression level of hypoxia-related proteins (hypoxia-inducible factor [HIF]-1α and Notch-1) and apoptosis-related proteins (B cell lymphoma [Bcl]-2, Bax, and caspase-3) were evaluated by Western blot analysis. Results: In response to hypoxia, cell viability was reduced dramatically, whereas the expression of HIF-1α was upregulated. Hypoxia also induced cardiomyocytes apoptosis by reducing the ratio of Bcl-2/Bax and increasing expression of caspase-3. Interestingly, Gen attenuated CoCl₂-induced cell death and suppressed HIF-1α expression, as well as upregulated the expression of Notch-1. Furthermore, Gen could antagonize CoCl₂-induced apoptosis through upregulating Bcl-2/Bax ratio and inhibiting caspase-3 expression. Conclusions: Gen prevents chemical hypoxia-induced cell apoptosis through inhibition of the mitochondrial apoptotic pathway, exerting protective effects on H9c2 cardiomyocytes.

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Section: Discussionmentioning

confidence: 99%

Genistein Protects H9c2 Cardiomyocytes against Chemical Hypoxia-Induced Injury via Inhibition of Apoptosis

Shi¹,

Zhang²,

Hu³

et al. 2019

Pharmacology

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“…These effects of diabetes in retinas were reduced by intervention treatment with genistein. Genistein, a tyrosine kinase inhibitor, represses the release of TNF-α and significantly inhibits ERK and P38 phosphorylation in activated microglial cells [118,157,158]. …”

Section: Natural Anti-diabetic/inflammatory Bioactive Compounds Anmentioning

confidence: 99%

Natural Phyto-Bioactive Compounds for the Treatment of Type 2 Diabetes: Inflammation as a Target

et al. 2016

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Diabetes is a metabolic, endocrine disorder which is characterized by hyperglycemia and glucose intolerance due to insulin resistance. Extensive research has confirmed that inflammation is closely involved in the pathogenesis of diabetes and its complications. Patients with diabetes display typical features of an inflammatory process characterized by the presence of cytokines, immune cell infiltration, impaired function and tissue destruction. Numerous anti-diabetic drugs are often prescribed to diabetic patients, to reduce the risk of diabetes through modulation of inflammation. However, those anti-diabetic drugs are often not successful as a result of side effects; therefore, researchers are searching for efficient natural therapeutic targets with less or no side effects. Natural products’ derived bioactive molecules have been proven to improve insulin resistance and associated complications through suppression of inflammatory signaling pathways. In this review article, we described the extraction, isolation and identification of bioactive compounds and its molecular mechanisms in the prevention of diabetes associated complications.

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“…Polyphenols are a group of naturally occurring compounds abundant in fruits, vegetables, and legumes and have been extensively investigated for their therapeutic benefits in several neurodegenerative disorders, mainly owing to their ability of resisting oxidative stress, modulating neuroinflammation, and influencing the process of apoptosis and autophagy (Manach, Scalbert, Morand, Remesy, & Jimenez, 2004;Ganai & Farooqi, 2015). Among the most studied polyphenol groups, Genistein (GE), which distributed predominantly in the Leguminosae family, has been reported to possess the maximum antioxidant activity (Sadhukhan, Saha, & Sil, 2016) and exhibit the antiinflammatory effects (Gupta et al, 2015). These beneficial effects of GE have been shown to be related to its neuroprotective potential both in vivo and in vitro studies against various neurodegenerative diseases (Schreihofer & Anthony, 2017;Sukanya, Pritam, Sushweta, Sayanta, & Parames, 2018;Ganai & Farooqi, 2015).…”

mentioning

confidence: 99%

Protective effects of Genistein on the cognitive deficits induced by chronic sleep deprivation

Lü

Jiang

et al. 2020

Phytotherapy Research

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Sleep deprivation has been widely reported to cause cognitive dysfunction, and elevation in oxidative stress and inflammation in the body, including the brain, have been suggested as the main factors. Genistein (GE) is an isoflavone widely present in leguminous plants, and it was found to exert a wide spectrum of biological activities, including antioxidant, anti‐inflammatory, hepatoprotective, neuroprotective, and antimetastatic effects. In this study, the protective effect of GE on chronic sleep deprivation (CSD)‐induced cognitive dysfunction was investigated. The mice were subjected to the sleep interruption apparatus and continuously sleep deprived for 25 days. GE was orally administrated (10, 20, and 40 mg/kg) during the sleep deprivation process totally for 25 days. Cognitive behavioral tests were conducted to study the learning and memory using the object location recognition (OLR) task, novel object recognition (NOR) test, and the Morris water maze (MWM) task. Additionally, the cortex and hippocampus were dissected to measure the oxidative stress markers and the antioxidant element nuclear erythroid‐2‐related factor 2 (Nrf2) and its downstream targets, including glutamate cysteine ligase catalytic, glutamate cysteine ligase modifier, heme oxygenase 1, and quinone oxidoreductase 1, as well as nuclear factor kappa B (NF‐κB) p65, nitric oxide synthase (iNOS), and cyclooxygenase 2 (COX‐2) protein expression. Moreover, the pro‐inflammatory cytokines (TNF‐α, interleukin [IL]‐6, and IL‐1β) level was examined in the serum. The current results showed that GE could dose‐dependently ameliorate the cognitive deficits of CSD‐treated mice in the OLR, NOR, and MWM tasks. In addition, GE treatment significantly elevated the activities of total antioxidant capacity and superoxide dismutase and the level of glutathione and lowered the content of malondialdehyde in the cortex and hippocampus of CSD‐treated mice. Furthermore, GE administration effectively activated the antioxidant element Nrf2 and its downstream targets in the cortex and hippocampus of CSD‐treated mice. Moreover, GE treatment significantly suppressed CSD‐induced NF‐κB p65, iNOS, and COX‐2 activation in the cortex and hippocampus, as well as inhibited CSD‐induced pro‐inflammatory cytokines (TNF‐α, IL‐6, and IL‐1β) release in the serum. Taken together, all these results suggested that GE has substantial potential as a therapeutic intervention for the alleviation of CSD‐induced deleterious effects.

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Genistein ameliorates cardiac inflammation and oxidative stress in streptozotocin-induced diabetic cardiomyopathy in rats

Cited by 94 publications

References 58 publications

Genistein Protects H9c2 Cardiomyocytes against Chemical Hypoxia-Induced Injury via Inhibition of Apoptosis

Genistein Protects H9c2 Cardiomyocytes against Chemical Hypoxia-Induced Injury via Inhibition of Apoptosis

Natural Phyto-Bioactive Compounds for the Treatment of Type 2 Diabetes: Inflammation as a Target

Protective effects of Genistein on the cognitive deficits induced by chronic sleep deprivation

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