Genistein down-modulates pro-inflammatory cytokines and reverses clinical signs of experimental autoimmune encephalomyelitis

Paula, Marcio L. De; Rodrigues, David Henrique; Teixeira, Henrique Couto; Barsante, Michele M.; Souza, Maria Aparecida de; Ferreira, Ana Paula

doi:10.1016/j.intimp.2008.05.002

Cited by 60 publications

(38 citation statements)

References 35 publications

(38 reference statements)

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“…Estrogens (Palaszynski et al, 2004;Niino et al, 2009) and estrogenic compounds, such as genistein (De Paula et al, 2008), are also receiving greater attention as potential anti-inflammatory agents for autoimmune diseases, such as multiple sclerosis. Employing the murine experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis, E2-mediated protection was significantly decreased in GPER knockout mice ), whereas G-1 treatment mediated an equivalent protection against the clinical and histologic manifestations of EAE to that of E2 (Blasko et al, 2009;Wang et al, 2009).…”

Section: Immune Systemmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. XCVII. G Protein–Coupled Estrogen Receptor and Its Pharmacologic Modulators

2015

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Section: Immune Systemmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. XCVII. G Protein–Coupled Estrogen Receptor and Its Pharmacologic Modulators

2015

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“…17 Thus far, there has been little research exploring the mechanism of GEN's action on neuroinflammation. It was reported that GEN has anti-inflammatory effects on human brain microvascular endothelial cells and BV-2 cells, 18,19 but it remains less clear whether GEN has similar neuroprotective effects against Ab-induced neuroinflammation.…”

Section: Introduction Amentioning

confidence: 99%

Genistein Alleviates β-Amyloid-Induced Inflammatory Damage Through Regulating Toll-Like Receptor 4/Nuclear Factor κB

Ding

et al. 2015

Journal of Medicinal Food

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Genistein (GEN), a major soybean isoflavone (SIF), might possess neuroprotective properties through its antiinflammatory activity. We hypothesized that GEN could prevent the inflammatory damage detected in C6 cells induced by b-amyloid peptides [25][26][27][28][29][30][31][32][33][34][35]. Accordingly, we evaluated the inflammatory damage induced by Ab25-35 and the protective effect of GEN against Ab25-35 in C6 cells. In our study, the C6 glial cells (rats glioma cell lines) were preincubated with or without GEN for 2 h following incubation with Ab25-35 for another 24 h. Then, methylthiazolyl tetrazolium (MTT) assay was used to assess the cell viability. Immunofluorescence staining was used to identify the C6 cells. Inflammatory factors tumor necrosis factor (TNF)-a and interleukin (IL)-1b were analyzed by using enzyme-linked immunosorbent assay (ELISA). Western blot analysis and reverse transcription-polymerase chain reaction analysis were performed to assess the expression of Toll-like receptors 4 (TLR4), inhibitor of kappaB-alpha (IjB-a). The current results showed that GEN could alleviate Ab25-35-induced cell apoptosis and prevent Ab25-35-induced TNF-a and IL-1b release from C6 cells. In addition, GEN prevented Ab25-35-induced upregulation of the gene and protein expression of TLR4, and GEN significantly upregulated the expression of IjB-a in C6 cells damaged by Ab25-35. These results suggest that GEN can alleviate the inflammatory stress caused by Ab25-35 treatment, which might be associated with the neuroprotective effect of GEN regulating the TLR4/NFjB signaling pathway.

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“…The relapsing and remitting form of MS is the most frequent disease course, although some patients develop progressive forms of the disease [5,6]. The etiology of MS is currently unknown and it has been extensively investigated with animal models [7,8].…”

Section: Introductionmentioning

confidence: 99%

Phytosterols ameliorate clinical manifestations and inflammation in experimental autoimmune encephalomyelitis

et al. 2010

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Genistein down-modulates pro-inflammatory cytokines and reverses clinical signs of experimental autoimmune encephalomyelitis

Cited by 60 publications

References 35 publications

International Union of Basic and Clinical Pharmacology. XCVII. G Protein–Coupled Estrogen Receptor and Its Pharmacologic Modulators

International Union of Basic and Clinical Pharmacology. XCVII. G Protein–Coupled Estrogen Receptor and Its Pharmacologic Modulators

Genistein Alleviates β-Amyloid-Induced Inflammatory Damage Through Regulating Toll-Like Receptor 4/Nuclear Factor κB

Phytosterols ameliorate clinical manifestations and inflammation in experimental autoimmune encephalomyelitis

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