Genistein Inhibits Intestinal Cell Proliferation in Piglets

Chen, An Chian; Berhow, Mark A.; Tappenden, Kelly A.; Donovan, Sharon M.

doi:10.1203/01.pdr.0000150723.87976.32

Cited by 33 publications

(26 citation statements)

References 58 publications

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“…Moreover, ERβ is the main form expressed in the piglets' intestine, while only very low expression of ERα was observed (Chen et al, 2005). Ac-cording to our findings both ERα and ERβ were expressed in the goblet cells of the fetal porcine intestine.…”

Section: Discussionsupporting

confidence: 81%

Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Knapczyk

Duda

Szafrańska

et al. 2008

Acta Veterinaria Hungarica

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The sites of oestrogen action can be shown by the localisation of their receptors in the target tissues. The aim of the present study was to show the localisation of oestrogen receptors in porcine embryos and fetuses obtained on days 18, 22, 32, 40, 50, 60, 71 and 90 post coitum (p.c.). The visualisation of proteins was conducted in embryos and various fetal organs such as gonads, uterus, lung, kidney, intestine and adrenal gland. Both ERs were observed in the blastocysts on day 18 p.c. In the male, ERβ was detected in the testis and epididymis, whereas ERα was present in the efferent ductules. In the female, ERβ was detected in the ovarian stromal cells investing the oocyte nests, while ERα protein was detected in the surface epithelium. In the uterus, ERs were present in the stromal cells, while ERβ was present in the luminal epithelium. In the non-reproductive fetal porcine tissues ERβ was localised in the lungs, kidneys, adrenal glands and in the umbilical cords. Both ERs were observed in the intestine. It is possible that ERβ may play important roles in the development of the adrenal gland, testis, kidney and lungs, while both ERs are involved in the development of the ovary, uterus, epididymis and intestine of the porcine fetus.

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Section: Discussionsupporting

confidence: 81%

Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Knapczyk

Duda

Szafrańska

et al. 2008

Acta Veterinaria Hungarica

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“…In comparison, plasma concentrations of isoflavones in adult pigs fed a typical soy-based diet reached approximately 1 M (14). However, piglets fed soy supplements in amounts typical for soy-infant formulas showed a serum concentration of genistein of 2.36 Ϯ 2.26 M on average, which was nearly identical to the mean serum concentration of 2.53 Ϯ 1.64 M in human infants (20). The piglet likely mimics the human infant's physiology and therefore represents a good model to investigate the effects of dietary isoflavones on the development of cells, tissues, and organs both in vivo and in vitro.…”

mentioning

confidence: 75%

“…Growing numbers of infants consume soy-based products and consequently are exposed to high concentrations of isoflavones (20). However, little attention has been directed toward possible effects of genistein and daidzein on postnatal growth performance or skeletal muscle development.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Isoflavones on Proliferation and DNA Integrity of Myoblasts Derived from Newborn Piglets

et al. 2008

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Soy-based formulas are consumed by growing numbers of infants and used as regular food supplements in livestock production. Moreover, constituent dietary phytoestrogens may compete with endogenous estrogens and affect individual growth. This study aimed to investigate the in vitro effects of isoflavones in comparison with estrogens on the proliferation of porcine satellite cells derived from neonatal muscle. After 7 h of exposure in serumfree medium, 17␤-estradiol (1 nM, 1 M), estrone (1 M), and daidzein (1, 100 M) slightly decreased whereas 100 M genistein substantially lowered DNA synthesis. Declines in DNA amount were observed with genistein (1, 100 M) and daidzein (100 M). After 26 h of exposure, 100 M genistein reduced DNA synthesis, whereas it was increased by 10 M genistein and 10 and 100 M daidzein. In the case of 10 M genistein and 100 M daidzein, these increases apparently resulted from the repair of damaged DNA. Genistein and daidzein (100 M) reduced protein synthesis, caused a G 2 /M phase block, and decreased DNA amount in association with higher rates of cell death partially resulting from apoptosis. Conclusively, isoflavones at concentrations of greater than 1 M act as inhibitors of porcine skeletal muscle cell proliferation.

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“…For instance, plasma concentrations of isoflavones in adult pigs fed a typical soy-based diet reached approximately 1 lM [5]. However, piglets fed soy supplements in amounts typical for soy-infant formulas showed a serum concentration of genistein, which was nearly identical to the mean serum concentration of 2.53 ± 1.64 lM in human infants, who were fed soy-based diets [44]. Moreover, supraphysiological (100 lM) concentrations of isoflavones were examined in the present study.…”

Section: Effects Of Isoflavones On Mrna Expression Of Igf-i Receptormentioning

confidence: 97%

Developmental changes and the impact of isoflavones on mRNA expression of IGF-I receptor, EGF receptor and related growth factors in porcine skeletal muscle cell cultures

Kalbe

Mau

Rehfeldt

2008

Growth Hormone & IGF Research

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Genistein Inhibits Intestinal Cell Proliferation in Piglets

Cited by 33 publications

References 58 publications

Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Effects of Dietary Isoflavones on Proliferation and DNA Integrity of Myoblasts Derived from Newborn Piglets

Developmental changes and the impact of isoflavones on mRNA expression of IGF-I receptor, EGF receptor and related growth factors in porcine skeletal muscle cell cultures

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