Genital Bovine Papillomavirus Infection in Saudi Arabia

Elzein, El Tayb Ebu; Sundberg, John P.; Housawi, F. M. T.; Gameel, A. A.; Ramadan, Ramadan O.; Hassanein, M. M.

doi:10.1177/104063879100300108

Cited by 21 publications

(12 citation statements)

References 5 publications

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“…In our studies, heifers had ages ranging from 1-2 years according to Maeda et al However, several authors verified papillomas both in young animals and in adults aged from 6 months to 10 years (Nooruddin et al;Elzein et al, 1991;Jelinek & Tachezy, 2005;Turk et al, 2005;Wosiacki et al, 2005;Borzacchiello et al, 2009;Silvestre et al, 2009;Özsoy et al).…”

Section: Discussionmentioning

confidence: 56%

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

et al. 2013

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SUMMARY:Papillomaviruses, belonging to the Papillomaviridae family, are small oncogenic viruses, causing papillomas and fibropapillomas in the mucosal and cutaneous epithelia of several animals. In bovine species, thirteen types (BPV 1-13) were characterized to date. In this study, the occurrence of papillomatosis in four outbreaks in cattle herds, coming from Brazilian states were registered. The papillomatous lesions were found located in the teats, udders, head and neck. Under the transmission electron microscope, by the negative staining technique, it was possible to visualized rounded-format papillomavirus, with icosahedral symmetry, characterized as "full" and "empty" particles, measuring on average 60 nm in diameter, in all the 40 samples observed of skin lesion fragments. The ultrathin sections revealed the presence of groups of viral, intranuclear, rounded particles measuring 35 nm in diameter and tubular particles with a diameter of 35-39 nm. At immunoelectron microscopy technique, positivity obtained was marked by the presence of aggregates of viral particles formed by the antigen-antibody interaction. In the immunocytochemistry technique, the antigen-antibody reaction showed colloidal gold particles evenly distributed over the surface of the virus. These results showed the importance of the transmission electron microscopy techniques in the diagnosis of bovine papillomatosis that can be used in routine procedures to identify viral agent of this important disease.

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Section: Discussionmentioning

confidence: 56%

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

et al. 2013

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“…Bovine papillomatosis has been reported in some Middle Eastern countries, such as Turkey and Saudi Arabia, where BPV-1 has been detected [28,29]. It has also been reported in Iran [30].…”

Section: Introductionmentioning

confidence: 96%

“…BPV diagnosis usually includes a clinical examination, histopathology, and immunohistochemistry [20,21]. Polymerase chain reaction (PCR) has been used as a sensitive method for the identification and genotyping of BPV [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq

et al. 2017

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Objective: This study aims to provide a molecular and epidemiological characterization of bovine papillomavirus (BPV) infections in Iraq. Methods: The present study focuses on identifying BPV based on clinical and epidemiological manifestations, histopathological examinations, and polymerase chain reactions (PCR). Samples were collected from 163 animals suffering from cutaneous bovine papillomatosis, including 129 females (79.14%) with an age range of 16–40 months and 34 males (20.85%) with an age range of 17–29 months. Results: The incidence rate was significantly higher in females than in males. The most commonly affected sites were the teats and neck, though warts were found in other areas of the body. Histological sections were diagnosed as fibropapilloma. PCR results showed that 80.13% of the extracted papilloma DNA samples corresponded to the BPV-1 genotype. Furthermore, 7.94% of the samples showed a mixed infection of BPV-1 and BPV-13. While, 40.63% of the extracted DNA blood samples showed 2 DNA fragments corresponding to both genotypes BPV-1 and BPV-2. Conclusions: This study confirmed the presence of BPV-1, BPV-2, and BPV-13, which belong to the Deltapapillomavirus genera, for the first time in the DNA of Iraqi cattle. Understanding BPV diversity and epidemiology is of critical importance for starting prevention strategies.

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“…The use of monoclonal antibodies against epitopes of BPV could also provide a diagnosis by immunohistochemistry (Elzein et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

et al. 2014

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ABSTRACT. Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic examination was done as a complementary and differential diagnosis. The fragments were purified, sequenced, and compared using BLASTn. The blood diagnosis showed the presence of BPV-2 and the analysis of cutaneous papillomas showed the presence of BPV-4, a new First description of BPV-12 and putative type BAPV8 in Brazil putative virus type BAPV8, and BPV-12, revealing for the first time the presence of BPV-12 and the putative type BAPV8 in beef cattle in Brazil. The sequences were deposited in the GenBank. Histopathology revealed acanthosis, hyperkeratosis, and koilocytosis in all samples analyzed. The presence of BAPV8 and BPV-12 in Brazil emphasizes the ubiquitous dissemination of BPVs in the herds of Brazil.

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Genital Bovine Papillomavirus Infection in Saudi Arabia

Cited by 21 publications

References 5 publications

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

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