Genital lesions and distribution of amastigotes in bitches naturally infected with Leishmania chagasi

Silva, Fabiana Lima; Rodrigues, Antônio; Rêgo, I.O.P.; Santos, Renato L.; Oliveira, Raquel G.; Silva, Teane M. A.; Xavier, Mariana N.; Nascimento, Evaldo

doi:10.1016/j.vetpar.2007.09.032

Cited by 38 publications

(45 citation statements)

References 22 publications

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“…In the present study, besides of these lesions, chronic inflammatory infiltrate was not restricted to the external genitalia and was also reported in the cervix and uterine tissue of the animals analyzed. Immunostaining detection for L. infantum amastigotes in the vulva and vagina is in agreement with data described by SILVA et al (2008), as these were the only organs to have amastigote forms of L. infantum in macrophages. According to the authors cited, previous injuries can attract parasitized macrophages to connective tissue of the vulva and vagina.…”

Section: Discussionsupporting

confidence: 91%

“…The structural changes in the vulva and vagina observed in the present investigation Ciência Rural, v.46, n.11, nov, 2016. resemble change reported in previous studies (SILVA et al, 2008), in which chronic inflammatory infiltrate has been considered the only lesion detected in these organs. Lesions were frequently reported next to hair follicles, sebaceous glands and sweat glands.…”

Section: Discussionsupporting

confidence: 89%

“…In male dogs, several studies have demonstrated the tropism of L. infantum in genital organs (DINIZ et al, 2005). However, few studies previous studies have demonstrated L. infantum tropism in female genital organs of the correlation between parasite load and the onset of lesions in these organs (DUBEY et al, 2005;ROSYPAL et al, 2005;SILVA et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…L. infantum amastigotes have been described in the uterus (ROSYPAL et al, 2005), placenta (DUBEY et al, 2005), vulva and vaginal mucosa. The presence of the parasite in the vulva and vaginal mucosa is related to multifocal chronic inflammatory infiltrate within macrophages, lymphocytes and plasma cells (SILVA et al, 2008;OLIVEIRA et al, 2012). However, SILVA et al (2008) detected no L. infantum amastigotes or lesions related to VL in the cervix, uterine body, uterine horn, uterine tubes and ovaries of naturally infected female dogs.…”

Section: Considerando a Falta De Estudos Sobre Lesões Nos óRgãos Genimentioning

confidence: 99%

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Correlation between chronic inflammation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum

et al. 2016

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The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum . Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions without immunostaining were observed in all organs, except the ovaries. L. Infantum DNA was detected in all organs of genital system, with no difference in parasite load observed among the different organs. No correlation was reported between parasite load and inflammatory lesions in the organs evaluated, except for the uterine body, in which an inverse correlation was detected. In conclusion, the vulva and vagina were the major sites of lesions and immunostaining for L. Infantum amastigotes in the genital system of female dogs. Moreover, parasite load exerted no influence on the intensity of the lesions in the organs evaluated.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Considerando a Falta De Estudos Sobre Lesões Nos óRgãos Genimentioning

confidence: 99%

See 2 more Smart Citations

Correlation between chronic inflammation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum

et al. 2016

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“…In dogs, VL is associated with variable clinical manifestations characterized by splenomegaly, limphadenopathy, anemia, pancytopenia, weight loss and weakness (Ciaramella et al, 1997). The disease may also cause male (Diniz et al, 2005) and female genital lesions (Silva et al, 2008).…”

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confidence: 99%

Detection of Leishmania infantum in the smegma of infected dogs

Silva

Assis

Ribeiro

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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Considering the venereal transmission of visceral leishmaniasis from dogs to bitches, the aim of this study was to verify if the penile surface and smegma from infected dogs can be the source of parasites in bitches. Twelve Leishmania infantum infected dogs had semen and smegma samples collected for submission to PCR identification of the DNA of the parasite. Semen (41.7%) and smegma (50.0%) have similar positive incidence (P>0.05; Fisher's exact test), with 58.3% of the dogs positive for semen and/or smegma samples. The proportion of positivity for both semen and smegma was 33.3%, but 8.3% was positive only for semen, and 16.7% only for smegma, revealing a moderate agreement between tests (K=0.5; Kappa index). It was concluded that Leishmania infantum is present in the smegma of contaminated dogs and it can be a source of parasites for the semen and the bitch.Keywords: Leishmania infantum; dog; prepuce, smegma; semen Palavras-chave: Leishmania infantum, cão, prepúcio, esmegma, sêmen RESUMO Tendo em vista a transmissão venérea da leishmaniose visceral do cão para a cadela, o objetivo deste estudo foi verificar se a superfície peniana e o esmegma de cães infectados poderiam ser a fonte de parasitas para a fêmea. Amostras de sêmen e esmegma de 12 cães infectados com

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Occurrence of Leishmania infantum and associated histological alterations in the genital tract and mammary glands of naturally infected dogs

et al. 2016

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The objectives of this study were to evaluate the occurrence of Leishmania infantum in the male and female genital tract and female mammary glands of dogs and the parasite burden and to identify histological alterations associated with this protozoan. Twenty male and 20 female Leishmania-seropositive dogs with isolation of L. infantum were examined. Tissue samples of the prepuce, glans, epididymis, testes, prostate, vulva, vagina, uterus, uterine tubes, and mammary glands were analyzed by immunohistochemistry and histopathology. For parasitological culture and in situ hybridization, samples were collected from the testis, epididymis, and uterus. Additionally, seminal fluid was aspirated from the epididymis for parasitological culture. In the genital tract, 34 (85 %) dogs, including 18 males and 16 females, were positive for Leishmania. Of these, 27 (79 %) animals were symptomatic. Leishmania was detected in the mammary glands of 13 (65 %) females. L. infantum was isolated for the first time from the seminal fluid and uterus of naturally infected dogs. The parasite burden and intensity of the inflammatory reaction were greater in the prepuce and glans of males and in the vulva and mammary glands of females. In addition to inflammation, testicular degeneration, atrophy, absence of spermatogenesis, and necrosis were observed. Detection of amastigote forms in the mammary gland lumen indicates possible elimination of this parasite in milk. The frequent parasitism observed in the genital tract of infected males and females and the viability of L. infantum in seminal fluid and uterus suggest the possibility of bidirectional venereal and vertical transmission.

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Genital lesions and distribution of amastigotes in bitches naturally infected with Leishmania chagasi

Cited by 38 publications

References 22 publications

Correlation between chronic inflammation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum

Correlation between chronic inflammation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum

Detection of Leishmania infantum in the smegma of infected dogs

Occurrence of Leishmania infantum and associated histological alterations in the genital tract and mammary glands of naturally infected dogs

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