Genital Tract Proteins in the Male Rabbit: II. Alkaline Phosphatase - Enzyme Action and Site of Synthesis

Abstract

Zusammenfassung Genitaltrakt‐spezifische Proteine des männlichen Kaninchens: II. Alkalische Phosphatase – Enzymatische Wirkung und histologische Lokalisation Für die Isolierung einer alkalischen Phosphatase durch Ionen‐Austausch‐Chromatographie, Gel‐Filtration und präparative isoelektrische Fokussierung wurde das Seminalplasma des Kaninchens verwendet. Enzym‐Inhibierungsexperimente mit Na2‐EDTA ergaben, daß Mg2+, Zn2+ und Ca2+ als Cofaktoren für die Enzymaktivität dienen, wobei Ca2+ den besten Effekt erzielte. Mit Hilfe eines monospezifischen Antiserums gegen die alkalische Phosphatase aus dem Seminalplasma konnten durch die Immun‐Fluoreszenz‐Technik als Syntheseort des Enzyms der Ductus epididymis, der Ductus deferens sowie die Ampullen des Ductus deferens gefunden werden.

Determination of enzyme activity in rabbit seminal plasma and its relationship with quality semen parameters

“…Values of LDH, AST and ALT activities obtained in this work are in the range of previous studies in rabbits (Yousef et al, 2003(Yousef et al, , 2004(Yousef et al, , 2005(Yousef et al, , 2010. However, in comparison with the results of Okab et al (2007), our ALKP, AST and ALT values are lower and our LDH values are higher.…”

“…This finding agrees with the results of Pesch et al (2006) in stallion, which showed a correlation of the enzymes GGT and ALKP and sperm concentration, as well as Kiso et al (2013) in elephant and Kozdrowski and Dubiel (2004) in boar, who reported a significant correlation between sperm concentration and seminal plasma ALKP activity. The main source of the ALKP in rabbits is the epididymis (Muller, 1983). Furthermore, seminal plasma ALKP activity is involved in sperm capacitation, acrosome reaction, hyperactivation, and zona pellucida binding (Salzberger et al, 1992;Urner and Sakkas, 2003).…”

“…Nevertheless, there are a limited number of studies focused on the presence of these enzymes in rabbit semen. Okab et al (2007) reported that the leakage of ALP and LDH in rabbit seminal plasma during summer and spring, respectively, could reveal a positive correlation between enzyme release and sperm cell integrity and acrosomal damage. In other works in rabbit, enzyme activity in seminal plasma has been studied to evaluate the effect of diet supplementation with different substances on semen quality (Yousef, 2005, Yousef et al, 2003, 2010Attia and Kamel, 2012).…”

Measurement of Alkaline Phosphatase in Canine Seminal Plasma – An Update

“…1993). AP activity is highest in the epididymis (Muller 1983; Frenette et al. 1986), incomplete ejaculation therefore results in AP activities ≤5000 IU / l, which can be used for diagnostic purposes (Freshman et al.…”

“…AP activity can be decreased after infectious, traumatic or thermal lesions of the epididymis, by autoimmune reactions or tumours (Allen and Renton 1982;Freshman et al 1988;Olson 1991;Olson et al 1992), as well as by obstruction or aplasia of efferent semen ducts (Althouse et al 1993). AP activity is highest in the epididymis (Muller 1983;Frenette et al 1986), incomplete ejaculation therefore results in AP activities £5000 IU ⁄ l, which can be used for diagnostic purposes (Freshman et al 1988;Johnston 1991;Johnston et al 2001).…”