Genome analyses of the wheat yellow (stripe) rust pathogen Puccinia striiformis f. sp. triticireveal polymorphic and haustorial expressed secreted proteins as candidate effectors

Cantu, Dario; Segovia, Vanesa; MacLean, Dan; Bayles, R. A.; Chen, Xianming; Kamoun, Sophien; Dubcovsky, Jorge; Saunders, Diane G. O.; Uauy, Cristóbal

doi:10.1186/1471-2164-14-270

Cited by 205 publications

(310 citation statements)

References 62 publications

Supporting

Mentioning

287

Contrasting

Order By: Relevance

“…Besides this, with the availability of the genome sequence of many rust pathogens, the prediction of effector candidates using structural and comparative analysis has increased significantly (Duplessis et al, 2011;Hacquard et al, 2011;Saunders et al, 2012;Cantu et al, 2013;Link et al, 2014;Nemri et al, 2014).…”

Section: Silencing Of Essential Pathogenic Genes For Pathogenicitymentioning

confidence: 99%

Asian soybean rust in Brazil: past, present, and future

Godoy

Seixas

Soares

et al. 2016

Pesq. agropec. bras.

173

199

View full text Add to dashboard Cite

-Asian soybean rust, caused by the fungus Phakopsora pachyrhizi, is the most severe disease of the crop and can cause yield losses of up to 90%. The disease was first reported in Brazil in 2001. Epidemics of the disease are common in the country, where the fungus can survive year-round. Regulatory measures to reduce the inoculum between seasons and avoid late-season soybean have been adopted to manage the disease. Disease control has relied mainly on chemical control, but a lower sensibility of the fungus to fungicides has been reported in Brazil. Major-resistance genes have been mapped and incorporated into the cultivars. With the reduced efficacy of the fungicides, the adoption of integrated measures to control the disease will be important for the sustainability of the crop. This review presents the main changes in the soybean crop system caused by the introduction of the fungus in Brazil, the current management strategies adopted to avoid losses, and the new trends that, together with biotechnological strategies, can improve management in the future.Index terms: Glycine max, Phakopsora pachyrhizi, biotechnology, chemical control, genetic resistance, losses, management. Ferrugem-asiática da soja no Brasil: passado, presente e futuroResumo -A ferrugem-asiática da soja, causada pelo fungo Phakopsora pachyrhizi, é a doença mais severa da cultura e pode causar perdas de produtividade de até 90%. A doença foi relatada pela primeira vez no Brasil em 2001. Epidemias da doença são comuns no País, onde o fungo pode sobreviver durante todo o ano. Medidas regulatórias para reduzir o inóculo entre safras e evitar a semeadura tardia de soja têm sido adotadas para manejar a doença. O controle da doença tem se baseado principalmente no controle químico, mas uma menor sensibilidade do fungo aos fungicidas tem sido relatada no Brasil. Genes de resistência têm sido mapeados e incorporados às cultivares. Por causa da redução da eficiência dos fungicidas, a adoção de medidas integradas para o controle da doença será importante para a sustentabilidade da cultura. Este artigo de revisão apresenta as principais mudanças no sistema de produção da soja causadas pela introdução do fungo no Brasil, as medidas de controle atualmente usadas para evitar perdas, e as novas tendências que, juntas com estratégias biotecnológicas, podem melhorar o manejo da doença no futuro.Termos para indexação: Glycine max, Phakopsora pachyrhizi, biotecnologia, controle químico, resistência genética, perdas, manejo.

show abstract

Section: Silencing Of Essential Pathogenic Genes For Pathogenicitymentioning

confidence: 99%

Asian soybean rust in Brazil: past, present, and future

Godoy

Seixas

Soares

et al. 2016

Pesq. agropec. bras.

173

199

View full text Add to dashboard Cite

show abstract

“…RNA sequencing (RNAseq) technology has become more frequently used to profile fungal pathogen gene expression at particular phases of plant infection (de Jonge et al, 2012;Kleemann et al, 2012;O'Connell et al, 2012;Soanes et al, 2012;Cantu et al, 2013;Garnica et al, 2013;Hacquard et al, 2013;Meinhardt et al, 2014;Zhang et al, 2014). However, comparatively few studies (Kawahara et al, 2012;Yang et al, 2013;Yazawa et al, 2013) have simultaneously analyzed the responses of both pathogen and host, and very few have undertaken such an analysis throughout a complete pathogen reproductive cycle.…”

mentioning

confidence: 99%

Transcriptome and Metabolite Profiling of the Infection Cycle ofZymoseptoria triticion Wheat Reveals a Biphasic Interaction with Plant Immunity Involving Differential Pathogen Chromosomal Contributions and a Variation on the Hemibiotrophic Lifestyle Definition

et al. 2015

View full text Add to dashboard Cite

The hemibiotrophic fungus Zymoseptoria tritici causes Septoria tritici blotch disease of wheat (Triticum aestivum). Pathogen reproduction on wheat occurs without cell penetration, suggesting that dynamic and intimate intercellular communication occurs between fungus and plant throughout the disease cycle. We used deep RNA sequencing and metabolomics to investigate the physiology of plant and pathogen throughout an asexual reproductive cycle of Z. tritici on wheat leaves. Over 3,000 pathogen genes, more than 7,000 wheat genes, and more than 300 metabolites were differentially regulated. Intriguingly, individual fungal chromosomes contributed unequally to the overall gene expression changes. Early transcriptional down-regulation of putative host defense genes was detected in inoculated leaves. There was little evidence for fungal nutrient acquisition from the plant throughout symptomless colonization by Z. tritici, which may instead be utilizing lipid and fatty acid stores for growth. However, the fungus then subsequently manipulated specific plant carbohydrates, including fructan metabolites, during the switch to necrotrophic growth and reproduction. This switch coincided with increased expression of jasmonic acid biosynthesis genes and large-scale activation of other plant defense responses. Fungal genes encoding putative secondary metabolite clusters and secreted effector proteins were identified with distinct infection phase-specific expression patterns, although functional analysis suggested that many have overlapping/redundant functions in virulence. The pathogenic lifestyle of Z. tritici on wheat revealed through this study, involving initial defense suppression by a slow-growing extracellular and nutritionally limited pathogen followed by defense (hyper) activation during reproduction, reveals a subtle modification of the conceptual definition of hemibiotrophic plant infection.

show abstract

“…hordei, C. fulvum and Puccinia striiformis f. sp. tritici, as well as the oomycetes Phytophthora infes tans and Hyaloperonospora arabidopsidis (Cantu et al, 2013;Liu et al, 2005;Pedersen et al, 2012;Stergiopoulos et al, 2007). Besides the prerequisite for secretion beyond the fungal cell, some secreted biomolecules that manipulate intracellular host function are required to be internalized.…”

Section: 21mentioning

confidence: 99%