Genome Analysis of Staphylococcus aureus ST291, a Double Locus Variant of ST398, Reveals a Distinct Genetic Lineage

Stegger, Marc; Aziz, Maliha; Chroboczek, Tomasz; Price, Lance B.; Ronco, Troels; Kiil, Kristoffer; Skov, Robert; Laurent, François; Andersen, Paal Skytt

doi:10.1371/journal.pone.0063008

Cited by 19 publications

(14 citation statements)

References 28 publications

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“…Thus, finding ST398 MSSA in the present sheep study was interesting, and these data suggest that ST398 MSSA may also be a sheep‐adapted strain, given the predominance of this strain here, including sheep with limited human contact. This is further supported by the lack of scn in the ST398 isolates, because this gene is strongly associated with the human ST398 clade . None of the farms in our study had pigs.…”

Section: Discussioncontrasting

confidence: 99%

“…This is further supported by the lack of scn in the ST398 isolates, because this gene is strongly associated with the human ST398 clade. 36 None of the farms in our study had pigs. This differs from the study by Gharsa et al,23 in which the ST398, both MRSA and MSSA, was thought to be due to cross-contamination from pigs.…”

Section: Discussionmentioning

confidence: 86%

“…Isolates were subjected to a sequence type 398 (ST398)‐specific PCR . PCR was also used to identify scn , a staphylococcal complement inhibitor that is strongly associated with the human ST398 clade …”

Section: Methodsmentioning

confidence: 99%

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Isolation of bacterial skin flora of healthy sheep, with comparison between frequent and minimal human handling

Haarstad¹,

Eisenschenk²,

Heinrich³

et al. 2014

Veterinary Dermatology

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 86%

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Isolation of bacterial skin flora of healthy sheep, with comparison between frequent and minimal human handling

Haarstad¹,

Eisenschenk²,

Heinrich³

et al. 2014

Veterinary Dermatology

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“…Although CC398-MSSA is prevalent among pigs and poultry in Europe, the evolution and global dispersal of this group have yet to be clarified (Hasman et al, 2010). The ST291/813 isolate forms, as a double locus variant of ST398, a distinct lineage within CC398 (Stegger et al, 2013). Although ST291 and ST813 yielded identical hybridization patterns, they clearly differed from the "normal" CC398 such as, for example, ST398 strains.…”

Section: Discussionmentioning

confidence: 99%

Staphylococci in cattle and buffaloes with mastitis in Dakahlia Governorate, Egypt

El-Ashker

Gwida

Tomaso

et al. 2015

Journal of Dairy Science

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The aim of this study was to provide the first detailed insight into the population structure of Staphylococcus aureus in one modern dairy farm (Gamasa) and several household cows and buffaloes in Dakahlia Governorate, Egypt. Eight hundred seventy-two quarter milk samples of 218 dairy cattle and buffaloes with clinical and subclinical mastitis were investigated. Bacteria were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and staphylococci were further characterized by DNA sequencing of 16S rRNA genes and microarray analysis. Staphylococcus aureus was present in 5.6% of all collected samples, whereas methicillin-resistant S. aureus (MRSA) represented 24.5% of all identified S. aureus (12/49). Six clonal complexes (CC) of S. aureus were detected. Staphylococcus aureus CC398 (ST291/813)-MSSA (methicillin-susceptible S. aureus) was identified frequently in the Gamasa farm in addition to a few CC5-MRSA-V isolates. However, a small number of different isolates of S. aureus were found in household cattle and buffaloes harboring different CC. The presence of these genotypes of S. aureus in milk might indicate a public health hazard, because all of these CC have previously been isolated from human patients. Thus, a recommendation was given to the owner of the dairy farm to review the hygiene regimen on the farm. In perspective, further investigation regarding S. aureus screening of all lactating cows and personnel on the farm is warranted.

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“…MSSA CC398 also remains present in animals, including pigs [32], dogs [14], bovines [29•], and poultry [29•, 33••]. Currently, CC398 includes 43 sequence types [34], but the major MRSA sequence type colonizing pigs is ST398. Other STs described in pigs are ST541, ST1965, ST1966, ST1967, and ST1968 [16,35,36].…”

Section: Cc398mentioning

confidence: 99%

Livestock-Associated MRSA and Its Current Evolution

Butaye

Argudín

Smith

2016

Curr Clin Micro Rpt

116

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a relatively recent phenomenon in veterinary medicine. Although in the beginning it was restricted to a single clonal complex (CC), CC398, it has expanded into several clonal complexes, and the diversity of subtypes in the clonal complexes is increasing also. The prevalence of each type is determined somewhat geographically; for instance, the most prevalent clonal complex in Europe is CC398, whereas in Asia, it is CC9. Although few data exist regarding North America, the situation appears to be mixed there. The SCCmec cassettes detected in LA-MRSA are limited mainly to SCCmec IVa and SCCmec V, although non-typeable cassettes and SCCmec type XI, containing mecC, also have been found.The source of the SCCmec in LA-MRSA was discovered to be animals. In searching from which bacteria the SCCmec cassettes in LA-MRSA have been transferred, the most obvious species to consider are the methicillin-resistant non-S. aureus staphylococci (MRNaS). However, very few data are available from those species in animals, and the data that do exist are not detailed enough to determine the origin. Nevertheless, similar cassettes were found in MRNaS, indicating a possible origin that needs to be investigated further.

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Genome Analysis of Staphylococcus aureus ST291, a Double Locus Variant of ST398, Reveals a Distinct Genetic Lineage

Cited by 19 publications

References 28 publications

Isolation of bacterial skin flora of healthy sheep, with comparison between frequent and minimal human handling

Isolation of bacterial skin flora of healthy sheep, with comparison between frequent and minimal human handling

Staphylococci in cattle and buffaloes with mastitis in Dakahlia Governorate, Egypt

Livestock-Associated MRSA and Its Current Evolution

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