Genome analysis revealed a repertoire of oligosaccharide utilizing CAZymes in Weissella confusa CCK931 and Weissella cibaria YRK005

Sharma, Neha; Gupta, Deepshikha

doi:10.1007/s10068-022-01232-7

Cited by 6 publications

(3 citation statements)

References 33 publications

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“…Weissella is closely related to Lactobacillus and produces high levels of lactic and acetic acids [ 61 ]. The purported health benefits of Weissella [ 62 ] are largely attributed to its ability to degrade several prebiotic oligosaccharides [ 63 , 64 ], and produce antimicrobial exopolysaccharides [ 65 , 66 , 67 ] favoring the growth of other probiotic species [ 68 , 69 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Sugar Beet Pulp on the Composition and Predicted Function of Equine Fecal Microbiota

Ford,

McAdams,

Townsend

et al. 2023

Biology

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The purpose of this study is to determine the effect of the partial replacement of dietary hay with sugar beet pulp (SBP) on the composition and predicted function of the fecal microbiota of healthy adult horses. Fecal samples were collected daily for 12 days from six adult horses after removal from pasture, including a five-day acclimation period, and a seven-day period following the introduction of SBP into their diet, and compared to six untreated horses over a comparable period. Fecal DNA was subjected to 16S rRNA amplicon sequencing and a longitudinal analysis was performed comparing the composition and predicted function. While no significant treatment-associated changes in the richness, alpha diversity, or beta diversity were detected, random forest regression identified several high-importance taxonomic features associated with change over time in horses receiving SBP. A similar analysis of the predicted functional pathways identified several high-importance pathways, including those involved in the production of L-methionine and butyrate. These data suggest that feeding SBP to healthy adult horses acutely increases the relative abundance of several Gram-positive taxa, including Cellulosilyticum sp., Moryella sp., and Weissella sp., and mitigates the predicted functional changes associated with removal from pasture. Large-scale studies are needed to assess the protective effect of SBP on the incidence of the gastrointestinal conditions of horses.

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Section: Discussionmentioning

confidence: 99%

Effect of Sugar Beet Pulp on the Composition and Predicted Function of Equine Fecal Microbiota

Ford,

McAdams,

Townsend

et al. 2023

Biology

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“…The L. lactis CCK940, L. lactis SBC001, and W. cibaria YRK005 strains have glycosyltransferases (GTs), such as maltose phosphorylase and dextransucrase, which produce oligosaccharides [ 57 , 58 ]. These strains also have glycoside hydrolase (GH) genes, which utilize oligosaccharides, such as β-galactosidase and α-glucosidase (EC 3.2.1.20).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, these strains have the β-galactosidase enzyme, which hydrolyzes lactose into glucose and galactose, as well as hydrolyzing a broad range of GOS (including oligosaccharides with 3–6 DP) [ 6 ]. An in silico analysis suggested that the three lactic acid bacterial strains contained enzymes synthesizing oligosaccharides [ 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Synergistic Immunostimulatory Activities of Probiotic Strains, Leuconostoc lactis and Weissella cibaria, and the Prebiotic Oligosaccharides They Produce

2023

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Synbiotics contain health-beneficial bacteria, i.e., probiotics and prebiotics selectively utilized by the probiotics. Herein, three probiotic strains, Leuconostoc lactis CCK940, L. lactis SBC001, and Weissella cibaria YRK005, and the oligosaccharides produced by these strains (CCK, SBC, and YRK, respectively) were used to prepare nine synbiotic combinations. Macrophages (RAW 264.7) were treated with these synbiotic combinations and the corresponding lactic acid bacteria and oligosaccharides alone to evaluate the treatments’ immunostimulatory activities. The level of nitric oxide (NO) production was significantly higher in the macrophages treated with the synbiotics than in those treated with the corresponding probiotic strains and the oligosaccharide alone. The immunostimulatory activities of the synbiotics increased regardless of the probiotic strain and the type of oligosaccharide used. The expressions of tissue necrosis factor-α, interleukin-1β, cyclooxygenase-2, inducible NO synthase genes, and extracellular-signal-regulated and c-Jun N-terminal kinases were significantly higher in the macrophages treated with the three synbiotics than in those treated with the corresponding strains or with the oligosaccharides alone. These results indicate that the synergistic immunostimulatory activities of probiotics and the prebiotics they produced in the studied synbiotic preparations resulted from the activation of the mitogen-activated protein-kinase-signaling pathway. This study suggests the combined use of these probiotics and prebiotics in the development of synbiotic preparations as health supplements.

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Biosynthesis of zinc oxide nanoparticles using the supernatant of Weissella cibaria UPM22MT06 and its antibacterial and cytotoxicity properties

Al-Tameemi,

Masarudin,

Abdul Rahim

et al. 2023

Biologia

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Genome analysis revealed a repertoire of oligosaccharide utilizing CAZymes in Weissella confusa CCK931 and Weissella cibaria YRK005

Cited by 6 publications

References 33 publications

Effect of Sugar Beet Pulp on the Composition and Predicted Function of Equine Fecal Microbiota

Effect of Sugar Beet Pulp on the Composition and Predicted Function of Equine Fecal Microbiota

Synergistic Immunostimulatory Activities of Probiotic Strains, Leuconostoc lactis and Weissella cibaria, and the Prebiotic Oligosaccharides They Produce

Biosynthesis of zinc oxide nanoparticles using the supernatant of Weissella cibaria UPM22MT06 and its antibacterial and cytotoxicity properties

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