Genome and Comparative Transcriptome Dissection Provide Insights Into Molecular Mechanisms of Sclerotium Formation in Culinary-Medicinal Mushroom Pleurotus tuber-regium

Abstract

Pleurotus tuber-regium is an edible and medicinal sclerotium-producing mushroom. The sclerotia of this mushroom also serve as food and folk medicine. Based on the description of its monokaryon genome, sequenced with Illumina and PacBio sequencing technologies, comparative transcriptomic analysis using RNA sequencing (RNA-seq) was employed to study its mechanism of sclerotium formation. The de novo assembled genome is 35.82 Mb in size with a N50 scaffold size of 4.29 Mb and encodes 12,173 putative proteins. Expression analysis demonstrated that 1,146 and 1,249 genes were upregulated and downregulated with the formation of sclerotia, respectively. The differentially expressed genes were associated with substrate decomposition, the oxidation-reduction process, cell wall synthesis, and other biological processes in P. tuber-regium. These genomic and transcriptomic resources provide useful information for the mechanism underlying sclerotium formation in P. tuber-regium.

Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects

“…The results of the multi-omics joint analysis can be corroborated with each other, which can help improve the scientificity and credibility of the study and will be more widely and deeply applied to the analysis of edible and medicinal fungi functional genes in the future. Sun et al [30] utilized the genome and transcriptome to gain insight into the molecular mechanism of nucleus formation in Pleurotus tuber-regium and reported that the genome size was 35.82 Mb and encoded 12,173 putative proteins. Of these, 1146 and 1249 genes were upregulated and downregulated, respectively, and it was hypothesized that these differential genes were related to substrate catabolism, oxidation-reduction process, cell wall synthesis, and other biological processes.…”