Genome Assembly and Annotation of<i>Botryosphaeria dothidea</i>sdau11-99, a Latent Pathogen of Apple Fruit Ring Rot in China

Chang, Yu; Diao, Yufei; Lü, Quan; Jun, Zhao; Cui, Shengnan; Peng, Cheng; He, Bang-Ling; Liang, Yan; Liu, Huixiang

doi:10.1094/pdis-06-20-1182-a

Cited by 4 publications

(1 citation statement)

References 31 publications

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“…This pathogen commonly causes fruit rot, warts, rough skin, and cankers on apple stems ( Li et al., 2009 ; Tang et al., 2012 ). With the release and availability of genome data ( Liu et al., 2016 ; Marsberg et al., 2017 ; Wang et al., 2018 ; Hu et al., 2019 ; Liang et al., 2021 ; Rao et al., 2021 ; Yu et al., 2021 ) and recently improved gene disruption methods ( Dong and Guo, 2020 ), research on gene function in B. dothidea is accelerating ( Dong et al., 2021 ; Zhang et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

LysM protein BdLM1 of Botryosphaeria dothidea plays an important role in full virulence and inhibits plant immunity by binding chitin and protecting hyphae from hydrolysis

Zhang,

Wen,

et al. 2024

Front. Plant Sci.

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Botryosphaeria dothidea infects hundreds of woody plants and causes a severe economic loss to apple production. In this study, we characterized BdLM1, a protein from B. dothidea that contains one LysM domain. BdLM1 expression was dramatically induced at 6 h post-inoculation in wounded apple fruit, strongly increased at 7 d post-inoculation (dpi), and peaked at 20 dpi in intact shoots. The knockout mutants of BdLM1 had significantly reduced virulence on intact apple shoots (20%), wounded apple shoots (40%), and wounded apple fruit (40%). BdLM1 suppressed programmed cell death caused by the mouse protein BAX through Agrobacterium-mediated transient expression in Nicotiana benthamiana, reduced H2O2 accumulation and callose deposition, downregulated resistance gene expression, and promoted Phytophthora nicotianae infection in N. benthamiana. Moreover, BdLM1 inhibited the active oxygen burst induced by chitin and flg22, bound chitin, and protected fungal hyphae against degradation by hydrolytic enzymes. Taken together, our results indicate that BdLM1 is an essential LysM effector required for the full virulence of B. dothidea and that it inhibits plant immunity. Moreover, BdLM1 could inhibit chitin-triggered plant immunity through a dual role, i.e., binding chitin and protecting fungal hyphae against chitinase hydrolysis.

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Section: Introductionmentioning

confidence: 99%

LysM protein BdLM1 of Botryosphaeria dothidea plays an important role in full virulence and inhibits plant immunity by binding chitin and protecting hyphae from hydrolysis

Zhang,

Wen,

et al. 2024

Front. Plant Sci.

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High-Quality Genome Assembly and Annotation Resource of Botryosphaeria dothidea Strain BDLA16-7, Causing Trunk Canker Disease on Chinese Hickory

Bao

Wu²,

Huang

et al. 2022

Plant Disease

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Botryosphaeria dothidea is a latent pathogen with global importance to woody plant health, which causes serious tree trunk cankers on Chinese hickory. To date, only one Illumina short-read-based genome assembly of strain CK16 is available for host Chinese hickory. To address this problem, we reported a near telomere-to-telomere genome assembly of strain BDLA16-7 (46.05 Mb, N50 3.87 Mb) using Oxford Nanopore Sequencing Technology. Our genome assembly was consisted of 15 contigs, of which, 3 were assembled into chromosomal level and the maximum contig length was 6.19 Mb. The assembly contained 7.96% repeats and 12,815 protein-coding genes (10,274 genes were functional annotated). We also identified 3,642 pathogen-host interaction (PHI) genes, 250 carbohydrateactive enzymes (CAZymes), 252 cytochrome P450 enzymes (CYPs), 752 putative secreted proteins and 63 secondary metabolite biosynthesis gene clusters (SMBGCs). The BUSCO completeness of genome assembly and predicted genes was 99.34% and 97.50%, respectively, at fungal level (n=758). The almost chromosomal-level and well-annotated genome assembly will provide a valuable genetic resource for understanding of the infection mechanisms of B. dothidea in future.

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Genome Assembly Resource and Annotation of Bursaphelenchus xylophilus TS-1, the Causal Agent of Pine Wilt Disease

Xiong

et al. 2023

PhytoFrontiers™

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Pine wilt disease is a major biological disaster caused by Bursaphelenchus xylophilus. This study establishes a high-quality, and well-annotated genome sequence of B. xylophilus strain TS-1 from Mountain Tai of Shandong province, China. The 75-Mbp assembly containing six chromosomes was established. This genome data represents a new valuable resource for future studies on B. xylophilus and the management of pine wilt disease.

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Genome Assembly and Annotation ofBotryosphaeria dothideasdau11-99, a Latent Pathogen of Apple Fruit Ring Rot in China

Cited by 4 publications

References 31 publications

LysM protein BdLM1 of Botryosphaeria dothidea plays an important role in full virulence and inhibits plant immunity by binding chitin and protecting hyphae from hydrolysis

LysM protein BdLM1 of Botryosphaeria dothidea plays an important role in full virulence and inhibits plant immunity by binding chitin and protecting hyphae from hydrolysis

High-Quality Genome Assembly and Annotation Resource of Botryosphaeria dothidea Strain BDLA16-7, Causing Trunk Canker Disease on Chinese Hickory

Genome Assembly Resource and Annotation of Bursaphelenchus xylophilus TS-1, the Causal Agent of Pine Wilt Disease

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