Genome based screening of epitope ensemble vaccine candidates against dreadful visceral leishmaniasis using immunoinformatics approach

An immunoinformatic approach driven by experimental proteomics: in silico design of a subunit candidate vaccine targeting secretory proteins of Leishmania donovani amastigotes

“…In recent years, several studies utilized immunoinformatic approaches of epitope screening in designing epitope-based vaccines. Khatoon et al [66], Singh et al [67] and Vakili et al [68] have previously reported the theoretical potential of in silico designed vaccines for visceral leishmaniasis. Notably, in a recent study by Vakili et al [69], the group further evaluated successfully the immunogenic potential of the multiepitope vaccine, derived in part from known antigens, by administering the chimeric construct in experimental mice.…”

“…This suggests that the in silico designed vaccines with epitopes derived from appropriate protein targets have the potential to progress toward advanced phases of vaccine development for visceral leishmaniasis. While the in silico studies by Khatoon et al [66] and Singh et al [67] largely utilized available genomic databases of L. donovani to select vaccine targets, Dikhit et al [11,70] performed thorough investigations involving in silico, in vitro and in vivo analysis to screen and validate immunogenic epitopes obtained from proteins that are increasingly expressed at the infective parasite stage. Such highly expressed proteins are likely important for physiological and/or infective process of the parasite and thus can be more effective vaccine targets.…”

An immunoinformatic approach driven by experimental proteomics: in silico design of a subunit candidate vaccine targeting secretory proteins of Leishmania donovani amastigotes

“…In recent years, several studies utilized immunoinformatic approaches of epitope screening in designing epitope-based vaccines. Khatoon et al [104], Singh et al [105] and Vakili et al [106] have previously reported the theoretical potential of in silico designed vaccines for visceral leishmaniasis. Notably, in a later study by Vakili et al [107], the group further evaluated successfully the immunogenic potential of the multi-epitope vaccine-derived in part from known antigens-by administering the chimeric construct in experimental mice.…”

“…This suggests that the in silico designed vaccines with epitopes derived from appropriate protein targets have the potential to progress toward advanced phases of vaccine development for visceral leishmaniasis. While the in silico studies by Khatoon et al [104] and Singh et al [105] largely utilized available genomic databases of L. donovani to select vaccine targets, Dikhit et al [11,108] performed thorough investigations involving in silico, in vitro and in vivo analysis to screen and validate immunogenic epitopes obtained from proteins that are increasingly expressed at infective stage of parasite. Such highly expressed proteins are likely important for physiological and/or infective process of the parasite and thus can be more effective vaccine targets.…”

An immunoinformatic approach driven by experimental proteomics: in silico design of a subunit candidate vaccine targeting secretory proteins of Leishmania donovani amastigotes

“…In recent years, several studies utilized immunoinformatic approaches of epitope screening in designing epitope-based vaccines. Khatoon et al[104], Singh et al[105] and Vakili et al[106] have previously reported the theoretical potential of in silico designed vaccines for visceral leishmaniasis. Notably, in a later study by Vakili et al[107], the group further evaluated successfully the immunogenic potential of the multiepitope vaccine, derived in part from known antigens, by administering the chimeric construct in experimental mice.…”