Genome classification of banana cultivars from South India using IRAP markers

Nair, A. Sreekumaran; Teo, Chee How; Schwarzacher, Trude; Heslop-Harrison, J. S.

doi:10.1007/s10681-005-7321-2

Cited by 62 publications

(34 citation statements)

References 16 publications

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“…The findings may imply that there is recombination between the A and B genomes. While the implication in previous studies (Heslop-Harrison & Schwarzacher, 2007;Howell, Newbury, Swennen, Whithers, & Ford-Lloyd, 1994;Nwakanma, Pillay, Okoli, & Tenkouano, 2003;Nair, Teo, & Schwarzacher, Heslop-Harisson, 2005) alluded marked differentiation of the A and B genomes, the SC3 primers developed in this study from the A genome do not suggest such a differentiation. Evidence for the lack of complete differentiation of the A and B genomes of banana does exist in the literature (Ortiz & Vuylsteke, 1994;Osuji, Harrison, Crouch, & Heslop-Harrison, 1997;D'Hont, Paget-Goy, Escoute, & Carreel, 2000;Khayat, 2004;Boonruangrod, Desai, Fluch, Berenyi, & Burg, 2008;Jeridi et al, 2011Jeridi et al, , 2012Cizkova et al, 2013;De Jesus et al, 2013).…”

Section: Discusscontrasting

confidence: 82%

“…Evidence for the lack of complete differentiation of the A and B genomes of banana does exist in the literature (Ortiz & Vuylsteke, 1994;Osuji, Harrison, Crouch, & Heslop-Harrison, 1997;D'Hont, Paget-Goy, Escoute, & Carreel, 2000;Khayat, 2004;Boonruangrod, Desai, Fluch, Berenyi, & Burg, 2008;Jeridi et al, 2011Jeridi et al, , 2012Cizkova et al, 2013;De Jesus et al, 2013). The first study that alluded to the lack of differentiation between the A and B genomes was that of (Nair, Teo, Schwarzacher, & Heslop-Harisson, 2005). They reached this conclusion when they found that there was no preferential pairing between the homologous chromosomes of the A genome in the plantain (AAB).…”

Section: Discussmentioning

confidence: 99%

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SCAR Marker for the A Genome of Bananas (Musa spp. L.) Supports Lack of Differentiation between the A and B Genomes

Mabonga¹,

Pillay²

2017

JAS

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Bananas (Musa spp. L.) are grouped on the basis of their genomic origins in relation to Musa acuminata (A genome) and M. balbisiana (B genome). The two ancestral wild seeded diploid species evolved in vastly different geographical areas and contributed several agronomic traits towards the present genetic composition of cultivated bananas. Most cultivated bananas are triploid (AAA, AAB and ABB), some are diploid (AA, BB and AB) and a few are tetraploids (AAAA, AAAB, AABB and ABBB). Limitations on the correct identification of the A and B genomes in Musa have generated need for the development of new and more reliable techniques. Distinguishing the A and the B genome remains practically and theoretically important for banana breeders. The aim of the research was to develop a DNA based A genome specific marker for the identification of the A genome in bananas. A putative marker (600 bp) specific to the A genome was identified by Random Amplified Polymorphic DNA (RAPD) technique. A sequence characterised amplified region (SCAR) marker was developed from the RAPD amplicon. The SCAR primers annealed a 500 bp fragment specific to the A genome in a sample of 22 randomly selected homo-and heterogenomic A genome containing accessions representing different genome combinations. The 500 bp SCAR marker is useful for the identification of the A genome. However an additional 700 bp fragment annealed in all M. balbisiana genotypes and in five of the eight heterogenomic accessions, suggesting lack of differentiation between the A and B genome. This study has provided a 500 bp A genome SCAR marker and recent evidence that the A and B genomes of banana may not be as differentiated as previously considered.

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Section: Discusscontrasting

confidence: 82%

Section: Discussmentioning

confidence: 99%

SCAR Marker for the A Genome of Bananas (Musa spp. L.) Supports Lack of Differentiation between the A and B Genomes

Mabonga¹,

Pillay²

2017

JAS

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“…In addition, they obtained the identical results from analyses carried out in two geographically distant laboratories. Nair et al (2005) used IRAP markers for the genome classification of banana varieties from South India. They observed that the genomic DNA of the 36 varieties showed multiple polymorphic bands with gypsi-IRAP primer.…”

Section: Resultsmentioning

confidence: 99%

Potato variety identification by molecular markers based on retrotransposon analyses

Nováková¹,

Šimáčková²,

Bárta³

et al. 2009

CZECH J GENET PLANT

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We analyzed a set of twenty most grown potato (Solanum tuberosum L.) varieties listed in the Czech Variety List using the PCR-IRAP (Inter-Retrotransposon Amplified Polymorphism) method in order to distinguish fast and unambiguously the varieties. In total, 62 polymorphic alleles were amplified using the three primers P-Tst-1, P-Tst-3 and P-Tst-6. The recorded pattern of markers was stable and reproducible. The analyses were repeated three times and identical results were always obtained. The level of polymorphism varied from 11% to 79% depending on the respective primer. All analysed varieties could be reliably distinguished after multivariate statistics have been applied to the data obtained by the PCO and UPGMA analyses. The best resolution of individual varieties was obtained if all three primers were evaluated as a complex. The use of retrotransposon-based markers appears to be suitable for the differentiation of large sets of potato samples and should be an eligible complement to other molecular markers used in potato variety identification such as Simple Sequence Repeats (SSR) and Amplified Fragment Length Polymorphisms (AFLP).

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“…IRAP markers have been lately utilized for characterization of plants such as Musa and Brassica species (Muhammad & Othman 2005;Nair et al 2005;Alix & Heslop-Harrison 2004). In the current study, IRAP data supports highly RAPD data analysis and they both come into agreement with the geographical facts of studied Eryngium L species in Syria.…”

Section: Discussionmentioning

confidence: 99%

Molecular and ecological study of Eryngium species in Syria

et al. 2010

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Eryngium L. species growing in Syria were characterized using morphological, geographical and molecular analyses (IRAP and RAPD). Eight Eryngium L. species have been determined to exist in Syria. E. glomeratum, E. campestre and E. falcatum were found to grow in the mountain regions. E. creticum and E. desertorum were found to grow in variant environments: mountains, semidesert and saline environments, which indicate their wide range of adaptation and tolerance to abiotic stresses. E. maritimum was the only species found to grow on the coastal sandy beaches suggesting that it is adapted to sandy, saline and humid environments. The two PCR-based techniques showed that E. pussilum and E. billardieri were the most distal to all other species. E. pussilum is found to grow in Leftaya region, which is a swamps area similar to the typical habitat for American Eryngiums. The most related species were E. glomeratum and E. campestre, which were mainly found in mountainous regions, followed by E. desertorum, E. falcatum, and E. creticum.

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Genome classification of banana cultivars from South India using IRAP markers

Cited by 62 publications

References 16 publications

SCAR Marker for the A Genome of Bananas (Musa spp. L.) Supports Lack of Differentiation between the A and B Genomes

SCAR Marker for the A Genome of Bananas (Musa spp. L.) Supports Lack of Differentiation between the A and B Genomes

Potato variety identification by molecular markers based on retrotransposon analyses

Molecular and ecological study of Eryngium species in Syria

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