Genome complexity of the maize rust fungus,Puccinia sorghi

Anderson, Peter; Tyler, Brett M.; Pryor, A.

doi:10.1016/0147-5975(92)90006-d

Cited by 10 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although it is possible that some other primer combinations or conditions could show greater sensitivity, our results show that DDRT-PCR has a strong bias towards higher abundance mRNAs. It is interesting to note that RAPD-PCR (12,13) which also uses lOmers of arbitrary sequence as primers for PCR with a complex genomic DNA as template, gives bands which mostly represent repetitive DNA (14,15).…”

mentioning

confidence: 99%

An analysis of differential display shows a strong bias towards high copy number mRNAs

Bertioli¹,

et al. 1995

View full text Add to dashboard Cite

mentioning

confidence: 99%

An analysis of differential display shows a strong bias towards high copy number mRNAs

Bertioli¹,

et al. 1995

View full text Add to dashboard Cite

“…Common rust (CR) of maize is widely distributed in temperate (Gingera, Davis, & Groth, 1994;Pataky & Tracy, 1999) and subtropical and tropical (Bekeko, 2019;Darino et al, 2016;Pratt & Gordon, 2006;Vivek et al, 2010) maize growing regions of the world. Puccinia sorghi is an obligate parasite (Anderson, Tyler, & Pryor, 1992;Pryor, 1994). Common rust disease epidemics are characterized with short latency periods of about five to ten days at temperatures of 15-25 • C and are more common at a relative humidity of at least 98% (Hooker, 1985;Pataky & Tracy, 1999).…”

Section: Crop Sciencementioning

confidence: 99%

Identification and diversity of tropical maize inbred lines with resistance to common rust (Puccinia sorghi Schwein)

et al. 2020

View full text Add to dashboard Cite

Common rust (CR) caused by Puccinia sorghi Schwein is one of the major foliar diseases of maize (Zea mays L.) in Eastern and Southern Africa. This study was conducted to (i) evaluate the response of elite tropical adapted maize inbred lines to Puccinia sorghi and identify resistant lines (ii) examine associations between CR disease parameters and agronomic traits, and (iii) assess the genetic diversity of the inbred lines. Fifty inbred lines were evaluated in field trials for three seasons (2017–2019) in Uganda under artificial inoculation. Disease severity was rated on a 1–9 scale at 21 (Rust 1), 28 (Rust 2), and 35 (Rust 3) days after inoculation. Area under disease progress curve (AUDPC) was calculated. The genetic diversity of the lines was assessed using 44,975 single nucleotide polymorphism markers. Combined ANOVA across seasons showed significant (P < .001) line mean squares for the three rust scores and AUDPC. Heritability was high for Rust 2 (0.90), Rust 3 (0.83), and AUDPC (0.93). Of the 50 lines, 12 were highly resistant to CR. Inbred lines CKL1522, CKL05010, and CKL05017 had significantly lower Rust 3 scores and AUDPC compared to the resistant check CML444 and are potential donors of CR resistance alleles. The genetic correlations between CR disease resistance parameters were positive and strong. A neighbor‐joining (NJ) tree and STRUCTURE suggested the presence of three major groups among the lines, with lines highly resistant to CR spread across the three groups. The genetic diversity among the highly resistant lines can be exploited by recycling genetically distant lines to develop new multiple disease resistant inbred lines for hybrid development and deployment.

show abstract

“…The ability to produce transgenic animals may be affected by a number of factors, among the most important being the quality and quantity of the DNA used for microinjection. Methods for the preparation of large DNAs such as BACs or PACs (1,2,(5)(6)(7)11,12) for microinjection have to date generally utilized purification of the DNA by ultracentrifugation through cesium chloride…”

Section: Bac and Pac Dna For The Generation Of Transgenic Animalsmentioning

confidence: 99%

Dry Grinding at Near-Ambient Temperatures for Extracting DNA from Rust and Other Fungal Spores

Zambino

2002

BioTechniques

View full text Add to dashboard Cite

Genome complexity of the maize rust fungus,Puccinia sorghi

Cited by 10 publications

References 16 publications

An analysis of differential display shows a strong bias towards high copy number mRNAs

An analysis of differential display shows a strong bias towards high copy number mRNAs

Identification and diversity of tropical maize inbred lines with resistance to common rust (Puccinia sorghi Schwein)

Dry Grinding at Near-Ambient Temperatures for Extracting DNA from Rust and Other Fungal Spores

Contact Info

Product

Resources

About