Genome damage and reactive oxygen species production in the progenies of irradiated CHO-K1 cells

Osipov, Andreyan N.; Lizunova, E. Yu.; Gur'ev, D. V.; Воробьева, Н. В.

doi:10.1134/s0006350911050149

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…Genome instability and senescence are not necessarily linked. Thus, immortalized cancer cell lines do not typically display senescence but their genomes are highly unstable [ 76 , 77 ]. Therefore, it is possible that in primary cultures of normal human cells senescence, but not genetic instability, is the main contributor to the observed effects.…”

Section: Discussionmentioning

confidence: 99%

Residual γH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells

Pustovalova

Астрелина

Grekhova

et al. 2017

Aging

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Mechanisms underlying the effects of low-dose ionizing radiation (IR) exposure (10-100 mGy) remain unknown. Here we present a comparative study of early (less than 24h) and delayed (up to 11 post-irradiation passages) radiation effects caused by low (80 mGy) vs intermediate (1000 mGy) dose X-ray exposure in cultured human bone marrow mesenchymal stem cells (MSCs). We show that γН2АХ foci induced by an intermediate dose returned back to the control value by 24 h post-irradiation. In contrast, low-dose irradiation resulted in residual γН2АХ foci still present at 24 h. Notably, these low dose induced residual γН2АХ foci were not co-localized with рАТМ foci and were observed predominantly in the proliferating Кi67 positive (Кi67+) cells. The number of γН2АХ foci and the fraction of nonproliferating (Кi67-) and senescent (SA-β-gal+) cells measured at passage 11 were increased in cultures exposed to an intermediate dose compared to unirradiated controls. These delayed effects were not seen in the progeny of cells that were irradiated with low-dose X-rays, although such exposure resulted in residual γН2АХ foci in directly irradiated cells. Taken together, our results support the hypothesis that the low-dose IR induced residual γH2AХ foci do not play a role in delayed irradiation consequences, associated with cellular senescence in cultured MSCs.

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Section: Discussionmentioning

confidence: 99%

Residual γH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells

Pustovalova

Астрелина

Grekhova

et al. 2017

Aging

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“…Long-term consequences following exposure to radiation are largely defined by cells ability to cope with DNA damage inflicted by irradiation. Although a broad spectrum of DNA lesions, including single- and double-strand breaks (DSBs), base modifications and sugar-phosphate backbone lesions, are typically induced by ionizing radiation, responses to DSBs have a major role in determining the fate of cells [ 24 – 28 ]. Normally, the cell response to ionizing radiation depends on the number of accumulated DSBs and may include processes, such as cell cycle arrest, activation of DNA repair and programs of cell death by autophagy or apoptosis [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Low doses of X-rays induce prolonged and ATM-independent persistence of γH2AX foci in human gingival mesenchymal stem cells

et al. 2015

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Interleukin-1β Can Reduce Manifestations of Delayed Effects of Prolonged Exposure to Low-Intensity γ-Radiation

et al. 2016

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We showed that injection of IL-1β (Betaleukin) in a dose of 3 μg/kg 22 h before prolonged (21 h) exposure to low-intensity (10 mGy/min) γ-radiation in a dose of 12.6 Gy reduced the number of double-strand DNA breaks in murine spleen cells to the control level in 4 months after exposure and the number of double-strand DNA breaks induced by additional acute irradiation in a dose of 6 Gy. The results suggest that IL-1β can improve the efficiency of systems reducing the number of double-strand DNA breaks in murine spleen cells at delayed terms after exposure to prolonged low-intensity radiation.

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Genome damage and reactive oxygen species production in the progenies of irradiated CHO-K1 cells

Cited by 4 publications

References 15 publications

Residual γH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells

Residual γH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells

Low doses of X-rays induce prolonged and ATM-independent persistence of γH2AX foci in human gingival mesenchymal stem cells

Interleukin-1β Can Reduce Manifestations of Delayed Effects of Prolonged Exposure to Low-Intensity γ-Radiation

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