Genome diversity at the serA-linked capsule locus in Escherichia coli

Boulnois, Graham J.; Drake, Rachel; Pearce, Rowan; Roberts, Ian S.

doi:10.1111/j.1574-6968.1992.tb05692.x

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…Region 2 is serotype specific and encodes the enzymes responsible for biosynthesis (where necessary) and polymerization of the individual monosaccharides that comprise the particular polysaccharide. The size of this region is variable; however, size is thought to reflect the complexity of the polysaccharide, as region 2 in isolates that produce CPS with complex structures often encodes a larger number of open reading frames [36]. Region 1 comprises 6 genes kpsFEDUCS organized in a single transcriptional unit ( fig.…”

Section: Genetic Organization and Regulation Of E Coli Group 2 Capsumentioning

confidence: 99%

Capsular Polysaccharides and Their Role in Virulence

Taylor

Roberts²

2004

Contributions to Microbiology

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Section: Genetic Organization and Regulation Of E Coli Group 2 Capsumentioning

confidence: 99%

Capsular Polysaccharides and Their Role in Virulence

Taylor

Roberts²

2004

Contributions to Microbiology

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“…This implies that group I/II capsule genes at serA are different from the group II capsule genes (2) and that in group I/II strains, the entire group II capsule gene cluster has been replaced by a different set of capsule genes (2,11). If this is true, then E. coli could have at least three distinct groups of gene clusters for capsular polysaccharide production, two of which map to a locus near serA (2).…”

mentioning

confidence: 99%

Cloning and analysis of gene clusters for production of the Escherichia coli K10 and K54 antigens: identification of a new group of serA-linked capsule gene clusters

Pearce

Roberts

1995

J Bacteriol

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The polysaccharide capsules of Escherichia coli have been classified into three groups: I, II, and I/II. The third group, I/II, has been poorly studied and possesses characteristics of both group I and group II capsules. In this report, we describe the cloning of the K10 and K54 capsule gene clusters, two representatives of group I/II capsules. Probes taken from DNA flanking regions 1 and 3 of the group II capsule clusters hybridized to these group I/II clones, confirming that the group I/II capsule genes are flanked by the same DNA and are therefore located in the same serA-linked region of the chromosome as group II capsule gene clusters. Southern blotting showed that homologous sequences were present in both the K10 and K54 capsule gene clusters and in other group I/II strains. No homology was detected between these sequences and the chromosomal DNA of either a group I or a group II strain. Likewise, no homology was detected to the chromosomal DNA of either a K11 or K19 strain, both of which had previously been classified as group I/II strains. In the K10 and K54 capsule gene clusters, these conserved sequences flanked a serotype-specific region in a manner analogous to group II capsule gene organization. Complementation of mutations in the kpsE, kpsD, and kpsC genes in region 1 of the K5 capsule gene cluster by subclones of the K10 and K54 capsule gene clusters indicated that certain stages in the export of group II and I/II capsules may be conserved. In the light of the findings presented here, we suggest that the group I/II capsule gene clusters are sufficiently different from group II capsule gene clusters to justify their renaming as group III.

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“…They are linear polymers and consist of multiple copies of regularly repeating subunits (Fig. Capsular polysaccharides also display enormous structural variations [16][17][18][19]. They are highly hydrated and can be substituted by non-sugar residues such as acetyl, acyl, phosphate and sulfate.…”

Section: Capsular Polysaccharide (Cps)mentioning

confidence: 99%

Current Understanding on Biosynthesis of Microbial Polysaccharides

Wang¹,

Guo²,

Song³

2008

CTMC

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The surfaces of almost all microbes are decorated with remarkable variations of polysaccharides such as O-antigen, capsular polysaccharides (CPS), and exopolysaccharides (EPS) in bacteria, lipoarabinomannans (LAM) in mycobacteria and lipophosphoglycan (LPG) in Leishmania. These polysaccharides play important roles in many biological processes, and they can function as the virulence determinants in the pathogens. The basic structures of these polysaccharides are known, but they show species-specificity or stage-specificity. For example, there are 186 O-serotypes and 80 capsular serotypes in E. coli. Despite the variation, the range of strategies used for the biosynthesis and assembly of these microbial polysaccharides is limited. Depending on the assembly and translocation mechanisms, O-antigen biosynthesis is subdivided into three pathways, of which the Wzy-dependent pathway is widely used not only in O-antigen, but also in CPS and EPS.

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Genome diversity at the serA-linked capsule locus in Escherichia coli

Cited by 4 publications

References 21 publications

Capsular Polysaccharides and Their Role in Virulence

Capsular Polysaccharides and Their Role in Virulence

Cloning and analysis of gene clusters for production of the Escherichia coli K10 and K54 antigens: identification of a new group of serA-linked capsule gene clusters

Current Understanding on Biosynthesis of Microbial Polysaccharides

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