Genome editing abrogates angiogenesis in vivo

Huang, Xionggao; Zhou, Guoqing; Wu, Wenyi; Duan, Yajian; Ma, Gaoen; Song, Jingyuan; Xiao, Ran; Vandenberghe, Luk; Zhang, Feng; D’Amore, Patricia A.; Lei, Hetian

doi:10.1038/s41467-017-00140-3

Cited by 125 publications

(131 citation statements)

References 41 publications

Supporting

Mentioning

126

Contrasting

Order By: Relevance

“…However, the HDP was non-zero even at normal incidence, especially when the light spot was near the edge of the sample. This cannot be explained by the CPGE mechanism reported in previous researches [8,18,19].…”

contrasting

confidence: 58%

Inverse spin Hall effect induced by asymmetric illumination of light in topological insulator Bi2Se3

Fan

Hobara

Akiyama

et al. 2020

Phys. Rev. Research

View full text Add to dashboard Cite

Using circularly polarized light is an alternative to electronic ways for spin injection into materials. Spins are injected at a point of the light illumination, and then diffuse and spread radially due to the in-plane gradient of the spin density. This diffusion is converted into a circular charge current by the inverse spin Hall effect (ISHE). With shining the circularly polarized light at asymmetric parts of the sample, such as near edges, we detected this current as a helicity-dependent component in the photocurrent. We present a model for this ISHE based on the experimental results and the finiteelement-method (FEM) simulation of the potential distribution induced by spin injection. Our model shows that the ISHE photocurrent generates an electric dipole at the edge of the sample, causing the measured charge current. The asymmetric lightillumination shown here is a simple way to inject and manipulate spins, opening up a door for novel spintronic devices.

show abstract

contrasting

confidence: 58%

Inverse spin Hall effect induced by asymmetric illumination of light in topological insulator Bi2Se3

Fan

Hobara

Akiyama

et al. 2020

Phys. Rev. Research

View full text Add to dashboard Cite

show abstract

“…The viral solution was concentrated with PEG 8000 and puri ed on a cesium chloride density gradient column. After two rounds of ultracentrifugation, the high-density viruses were separated and extracted, and run through dialysis bags for desalting [30]. The titers of the puri ed rAAV-DJ/8, rAAV9 viruses were determined with a RT-PCR-based method described previously [31].…”

Section: Methodsmentioning

confidence: 99%

A Newly Built rAAV-SaCas9 Genome Editing System Enables Muscle-Directed Gene Editing to Improve Muscular Atrophy

Weng¹,

Li²,

Zhao³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background At present, genome editing at specific sites in vivo is affected by many factors, including the choice of vector, the efficiency of editing proteins and the influence of the internal environment. These factors make gene editing ineffective and even have adverse effects. Methods Here, we report a single rAAV containing SaCas9 and guide RNAs under the control of subtle EF1a and tRNA promoters. The capacity of rAAV was compressed, and we inserted the sequence of the green fluorescent protein eGFP into rAAV. The efficiency of rAAV gene editing in vivo and in vitro was analyzed by time point and virus titer. In addition, we used the rAAV9-SaCas9 system to knock out the myostatin gene in the thigh muscles of muscle-atrophic mice. Results We demonstrated that the gene editing elements regulated by the rAAV-SaCas9 system can be expressed. By increasing the amount of rAAV and the reaction time, the editing efficiency of myostatin and the expression level of eGFP protein can be improved in vitro and vivo. Furthermore, We demonstrated that muscle cells were improved by knockout partial myostatin gene in a mouse model of muscular dystrophy. Conclusions The rAAV-SaCas9 system can be expressed in a stable and long-term manner. The system has substantial therapeutic potential in treating muscular atrophy.

show abstract

“…level, downregulate inflammation and protect against sepsis . As a novel gene editing technique which has been applied in the eye (Huang et al 2017), the CRISPR/Cas9 system could also be used to deplete IL-17 in the ocular disease model in both biological study and future clinical applications (Peng et al 2017a). Besides, as IL-17 is an important cytokine connected with many kinds of cells (such as macrophages, Th cells, and γδT cells), cell therapy, including stem cells such as induced pluripotent stem cells (iPSCs) (Dabrowska and Skopinski 2017), might also be a promising option combined with targeting IL-17.…”

Section: Potential Applications and Therapies Of Il-17 For Ocular Neomentioning

confidence: 99%

Interleukin-17: The Role for Pathological Angiogenesis in Ocular Neovascular Diseases

Yuan-jun

Zhou

2019

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

Ocular neovascular diseases are featured by abnormal angiogenesis in the eye, and they seriously threaten the human visual health. These diseases include proliferative diabetic retinopathy (PDR), age-related macular degeneration (AMD), retinopathy of prematurity (ROP), and retinal vein occlusion (RVO). In fact, ocular neovascular diseases represent the leading causes of vision impairment and blindness worldwide. Ocular neovascularization, the process of pathological vessel formation in eye, underlies ocular neovascular diseases. Cytokines have important regulatory roles in neovascularization through immunological networks. Interleukin (IL)-17, the signature cytokine produced by T helper 17 (Th17) cells, has proven to be involved in ocular neovascularization. However, roles of IL-17 in ocular neovascular diseases still remain controversial. This review provides an overview of the functional roles of IL-17 in ocular neovascular diseases from basic research to clinical evidence by focusing on PDR, AMD, ROP, and RVO. The possible roles of IL-17 in neovascularization are achieved through a regulatory network of cytoskeleton remodeling, vascular endothelial growth factor (VEGF), VEGF-related cytokines, and complement components. Current applications as well as potential therapies targeting IL-17 with genome editing systems are also outlined and discussed. Targeting IL-17 might be a promising therapeutic strategy against ocular neovascular diseases.

show abstract

Genome editing abrogates angiogenesis in vivo

Cited by 125 publications

References 41 publications

Inverse spin Hall effect induced by asymmetric illumination of light in topological insulator Bi2Se3

Inverse spin Hall effect induced by asymmetric illumination of light in topological insulator Bi2Se3

A Newly Built rAAV-SaCas9 Genome Editing System Enables Muscle-Directed Gene Editing to Improve Muscular Atrophy

Interleukin-17: The Role for Pathological Angiogenesis in Ocular Neovascular Diseases

Contact Info

Product

Resources

About