Genome editing

McGrail, Maura; Sakuma, Tetsushi; Bleris, Leonidas

doi:10.1038/s41598-022-24850-x

Cited by 1 publication

(1 citation statement)

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“…In utero electroporation (IUE), a kind of in vivo gene delivery method has been widely applied to such visualization and functional studies in developing and postnatal brains [ 8 , 9 , 10 ]. The recent advent of advanced techniques such as spatiotemporal gene regulation [ 11 ] and genome editing [ 12 , 13 ] have been applied to IUE and further expanded our investigative toolbox. In addition to IUE, the iGONAD method, a newly developed genome-editing approach by intragonadal electroporation, is an accelerated method to explore brain development [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Advanced Techniques Using In Vivo Electroporation to Study the Molecular Mechanisms of Cerebral Development Disorders

Yang,

Shitamukai,

Yang

et al. 2023

IJMS

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The mammalian cerebral cortex undergoes a strictly regulated developmental process. Detailed in situ visualizations, imaging of these dynamic processes, and in vivo functional gene studies significantly enhance our understanding of brain development and related disorders. This review introduces basic techniques and recent advancements in in vivo electroporation for investigating the molecular mechanisms underlying cerebral diseases. In utero electroporation (IUE) is extensively used to visualize and modify these processes, including the forced expression of pathological mutants in human diseases; thus, this method can be used to establish animal disease models. The advent of advanced techniques, such as genome editing, including de novo knockout, knock-in, epigenetic editing, and spatiotemporal gene regulation, has further expanded our list of investigative tools. These tools include the iON expression switch for the precise control of timing and copy numbers of exogenous genes and TEMPO for investigating the temporal effects of genes. We also introduce the iGONAD method, an improved genome editing via oviductal nucleic acid delivery approach, as a novel genome-editing technique that has accelerated brain development exploration. These advanced in vivo electroporation methods are expected to provide valuable insights into pathological conditions associated with human brain disorders.

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