Genome Editing for Tomato Improvement

Summary Female inflorescence is the primary output of medical Cannabis. It contains hundreds of cannabinoids that are accumulated in the glandular trichomes. However, little is known about the genetic mechanisms governing Cannabis inflorescence development. In this study, we reported the map-based cloning of a gene determining the number of inflorescences per branch. We named this gene CsMIKC1 since it encodes a transcription factor that belongs to the MIKC-type MADS subfamily. Constitutive overexpression of CsMIKC1 increases inflorescence number per branch, thereby promoting the flower production as well as grain yield in transgenic Cannabis plants. We further identified a plant-specific transcription factor CsBPC2, promoting the expression of CsMIKC1. The CsBPC2 mutants and CsMIKC1 mutants were successfully created using CRISPR-Cas9 system, which exhibited similar inflorescence degeneration and grain reduction. We also validated the interaction of CsMIKC1 with CsVIP3, which suppressed expression of four inflorescence development-related genes in Cannabis. Our findings establish important roles for CsMIKC1 in Cannabis, which could represent a previously unrecognized mechanism of inflorescence development regulated by ethylene.

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Genome Editing for Tomato Improvement

Cited by 2 publications

References 63 publications

Transcriptional regulation of tomato fruit ripening

Transcriptional regulation of tomato fruit ripening

CsMIKC1 regulates inflorescence development and grain production in Cannabis sativa plants

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