Genome Editing: Methods and Application in Plant Pathology

Yadav, Lokesh; Kapoor, Promil; Kumar, Ashwani

doi:10.20546/ijcmas.2019.805.149

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2021

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“…However, this is an underused tool due to its limitations, such as recognizing few DNA sequences and the possibility of errors occurring due to insertions or deletions at the cleavage site (Daboussi et al 2015, Majid et al al. 2017, Yadav et al 2019, Yin and Qiu 2019.…”

Section: Mnsmentioning

confidence: 99%

Rice blast: strategies and challenges for improving genetic resistance

Nizolli

Pegoraro

Oliveira

2021

Crop Breed. Appl. Biotechnol.

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Rice blast, caused by the fungus Pyricularia oryzae L., is considered one of the main threats to world rice production. The development of resistant cultivars is one of the best and sustainable control alternatives. Plant breeding efforts have been accelerated by genetic mapping (linkage and associative) and marker assisted selection. On the other hand, genomic editing techniques, such as meganucleases (MNs), Zinc-finger nucleases (ZFNs), Transcription Activator-like Effector Nucleases (TALENs) and Clustered Regularly Interspaced Short Palindrome Repeats/CRISPR-associated protein 9 (CRISPR/Cas9), can be used to promote specific genetic modifications. Likewise, transgenics can also be used to manipulate specific genes. In this sense, this work aims to characterize rice blast and elucidate available biotechnological alternatives to accelerate the development of improved rice cultivars resistant to rice blast.

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Section: Mnsmentioning

confidence: 99%