Genome editing of apple <i>SQUAMOSA PROMOTER BINDNG PROTEIN-LIKE 6</i> enhances adventitious shoot regeneration

Li, Huixia; Sun, Haochen; Dong, Haiqiang; Wang, Shiyao; Fan, Xingqiang; Li, Yi; Cheng, Lailiang; Zhang, Zhihong; Wang, Yi; Zhang, Xinzhong; Xu, Xuefeng; Han, Zhenhai; Li, Wei

doi:10.1093/plphys/kiac570

Cited by 4 publications

(2 citation statements)

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“…Increased shoot regeneration [33] Apple is a valuable fruit crop, with yearly global apple production in 2021 reaching 9.4 million tons [1]. It would be advantageous to improve resistance to key biotic stresses, including fire blight and apple scab, as these factors severely impact tree health and decrease marketable fruit yield.…”

Section: Mdspl6mentioning

confidence: 99%

“…There is interest in developing approaches in many other fruit species, including tropical fruits, but progress is hampered by a need for improved genetic resources, micropropagation, gene transfer abilities, and more [32]. A recent study in apple found that editing the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 6 (MdSPL6) gene led to increased shoot formation in tissue culture; perhaps this approach could be applied to challenging species or cultivars [33]. For most proof-of-concept studies, the gene PHYTOENE DESATURASE (PDS) was targeted, as the inactivation of gene function leads to the development of albino cells, which can be readily visualized during the regeneration process.…”

Section: Introductionmentioning

confidence: 99%

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Apple CRISPR-Cas9—A Recipe for Successful Targeting of AGAMOUS-like Genes in Domestic Apple

Jacobson,

Bondarchuk,

Nguyen

et al. 2023

Plants

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Fruit trees and other fruiting hardwood perennials are economically valuable, and there is interest in developing improved varieties. Both conventional breeding and biotechnology approaches are being utilized towards the goal of developing advanced cultivars. Increased knowledge of the effectiveness and efficiency of biotechnology approaches can help guide use of the CRISPR gene-editing technology. Here, we examined CRISPR-Cas9-directed genome editing in the valuable commodity fruit tree Malus x domestica (domestic apple). We transformed two cultivars with dual CRISPR-Cas9 constructs designed to target two AGAMOUS-like genes simultaneously. The main goal was to determine the effectiveness of this approach for achieving target gene changes. We obtained 6 Cas9 control and 38 independent CRISPR-Cas9 events. Of the 38 CRISPR-Cas9 events, 34 (89%) had gene edits and 14 (37%) showed changes to all alleles of both target genes. The most common change was large deletions, which were present in 59% of all changed alleles, followed by small deletions (21%), small insertions (12%), and a combination of small insertions and deletions (8%). Overall, a high rate of successful gene alterations was found. Many of these changes are predicted to cause frameshifts and alterations to the predicted peptides. Future work will include monitoring the floral development and floral form.

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Section: Mdspl6mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%