Genome editing of FTR42 improves zebrafish survival against virus infection by enhancing IFN immunity

Protocol for linking enhanced interferon immunity to virus resistance in gene-knockout zebrafish

“… 6 We thus propose that, to appropriately promote interferon immunity in fish, just toward virus infection but not in normal conditions, might be essential for fish survival against virus infection. 1 , 7 , 8 …”

“…Recently we have generated a FTR42 gene-knockout zebrafish, showing an improved resistance to SVCV infection, with a normal phenotype in growth, reproduction and feeding. 1 In vitro assays indicate that zebrafish FTR42, a finTRIM (fish novel TRIM, FTR) family member that is homologous to FTRCA1 (FTR Carassius auratus 1) in crucian carp, 8 , 9 acts as an inhibitor of interferon response. 1 However, it is obvious that in vivo evidence is important for clarifying the genetical basis on the improved resistance to virus infection in FTR42-knockout zebrafish.…”

“… Note: E3-mut, a FTR42 mutant with a deficient in E3 ligase activity, is derived from the FTR42 expression plasmid. 1 Verify these plasmids by sequencing. Extract and purify plasmid DNAs using the Endo-free Plasmid Mini Kit II (OMEGA).…”

“…Note: E3-mut, a FTR42 mutant with a deficient in E3 ligase activity, is derived from the FTR42 expression plasmid. 1 …”

“… 1 N/A IRF7 (expression plasmid) Qu et al. 1 N/A FTR42 (expression plasmid) Qu et al. 1 N/A E3-mut (expression plasmid) Qu et al.…”