Genome Editing Using the CRISPR-Cas9 System to Generate a Solid-Red Germline of Nile Tilapia (<i>Oreochromis niloticus</i>)

Segev-Hadar, Adi; Slosman, Tatiana; Rozen, Ada; Sherman, Amir; Cnaani, Avner; Biran, Jakob

doi:10.1089/crispr.2020.0115

Cited by 31 publications

(18 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A recent study describes the generation of stable and heritable red tilapia phenotype through induced loss-of-function mutations in the slc45a2 gene of Nile tilapia ( Oreochromis niloticus ) [ 102 ]. The solute carrier family 45 member 2 ( slc45a2 ) is a membrane-associated transporter protein that mediates melanin biosynthesis and is evolutionarily conserved from fish to humans.…”

Section: Gene Editing In Fish Farm Species Using Crispr/cas9 and Othe...mentioning

confidence: 99%

See 1 more Smart Citation

Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine

Gutási

Hammer

El-Matbouli

et al. 2023

Animals

View full text Add to dashboard Cite

Gene editing and gene silencing techniques have the potential to revolutionize our knowledge of biology and diseases of fish and other aquatic animals. By using such techniques, it is feasible to change the phenotype and modify cells, tissues and organs of animals in order to cure abnormalities and dysfunctions in the organisms. Gene editing is currently experimental in wide fields of aquaculture, including growth, controlled reproduction, sterility and disease resistance. Zink finger nucleases, TALENs and CRISPR/Cas9 targeted cleavage of the DNA induce favorable changes to site-specific locations. Moreover, gene silencing can be used to inhibit the translation of RNA, namely, to regulate gene expression. This methodology is widely used by researchers to investigate genes involved in different disorders. It is a promising tool in biotechnology and in medicine for investigating gene function and diseases. The production of food fish has increased markedly, making fish and seafood globally more popular. Consequently, the incidence of associated problems and disease outbreaks has also increased. A greater investment in new technologies is therefore needed to overcome such problems in this industry. To put it concisely, the modification of genomic DNA and gene silencing can comprehensively influence aquatic animal medicine in the future. On the ethical side, these precise genetic modifications make it more complicated to recognize genetically modified organisms in nature and can cause several side effects through created mutations. The aim of this review is to summarize the current state of applications of gene modifications and genome editing in fish medicine.

show abstract

Section: Gene Editing In Fish Farm Species Using Crispr/cas9 and Othe...mentioning

confidence: 99%

“…( C ) shows slc45a2 -RNPs-injected mutant fish with 97–99% loss of melanin in the skin and no melanin in the eye. ( D ) shows post sexual maturation, F0 mutant displaying a complete loss of melanin [ 102 ].…”

Section: Figurementioning

confidence: 99%

Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine

Gutási

Hammer

El-Matbouli

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

“…Because the Tyrosine gene play a role in catalyzed the melanin biosynthesis in pigment cells and also determined vertebrate coloration. In Nile Tilapia (Oreochromis niloticus) [32], the slc45a2 gene was knocked out, which mediated melanin biosynthesis and produced an albino variant with red skin and eyes. In Chinese lamprey (Lethenteron morii) [45], the slc45a5 gene was knocked out, which resulted in the 'Golden phenotype' of the fish.…”

Section: Pigmentationmentioning

confidence: 99%

CRISPR Genome Editing Technology to Revolutionize Aquaculture and Fisheries

Moorthi¹,

P²,

Naduvathu³

2023

Act Sci VetSci

View full text Add to dashboard Cite

Aquaculture is rapidly replacing wild fish with farmed fish as the principal source of seafood in human diets. Diseases, reduced viability, fertility decline, poor growth, farmed fish escape into wild, and environmental pollution are the major issues faced by the sector. The commercialization of AquaAdvantage salmon and CRISPR/Cas9-developed tilapia (Oreochromis niloticus) initiated using of genetic engineering and genome editing methods, such as CRISPR/Cas, as potential solutions to overcome the challenges. Disease resistance, sterility, and improved growth are among the future features being developed in many fish species. CRISPR/Cas is less expensive, simpler, and more precise than existing genome editing technologies, and it can be employed as a new breeding method in fisheries and aquaculture to address major difficulties. Furthermore, unlike transgenesis, which introduces foreign genes into the host genome and thus alleviates major public safety concerns, CRISPR/Cas genome editing rapidly introduces favourable changes by disrupting genes with targeted minor changes. Although CRISPR/Cas technology has enormous potential, there are a number of technical hurdles as well as regulatory and public issues involving its usage in fisheries and aquaculture. Nonetheless, the exciting point in the CRISPR/Cas9 genome editing is that two CRISPR-edited fish, namely, red sea bream and tiger puffer developed by the Kyoto-based startup, have received approval and are now available for purchase, while another fish, FLT-01 Nile tilapia developed by AquaBounty, is not classified as a genetically modified organism regulatory. However, it is still a long way from revolutionizing and becoming a viable commercial aquaculture breeding technology for aquaculture-important features and species.

show abstract

“…The DSB can be repaired by two major DNA repair pathways, namely, nonhomologous end joining (NHEJ) and homology-directed repair (HDR). The NHEJ pathway repairs two adjacent strands of DNA that are error-prone and usually induce unpredictable insertion or deletions (indels) and substitutions (Segev-Hadar et al, 2021). Additionally, if a repair template is present, another pathway HDR can be used to insert the desired mutations for precise gene editing (Figure 1).…”

Section: What Is Crispr and How Does It Work?mentioning

confidence: 99%

“…Recently, there is an increasing market demand for red tilapia that are the hybrids of different tilapia species or the red variant of the highly inbred Nile tilapia, and this red-color phenotype is unstable, resulting in a nonuniform coloration in appearance or dark-red color blotches that reduce the market value. Hence, the CRISPR/Cas9 system was used to target the slc45a2 gene in melanin biosynthesis and produces an albino variant of Nile tilapia with red skin and eyes (Segev-Hadar et al, 2021). Additionally, researchers are utilizing slc family genes to improve the visual confirmation of knockout fish or strain, and in such a research, Pandey et al (2021) showed that slc2415 mutants showed reduced melanin pigmentation in the retina and bodies of Kawakawa (Euthynus affinis), highlighting toward a future where the visual recognition of a genome-edited stock will be possible.…”

Section: Pigmentationmentioning

confidence: 99%

CRISPR/Cas Genome Editing—Can It Become a Game Changer in Future Fisheries Sector?

Roy

Kumar²,

Behera³

et al. 2022

Front. Mar. Sci.

View full text Add to dashboard Cite

Fisheries and aquaculture are the fastest-growing food-producing sector and rapidly becoming an important element for the global food security since they are the primary source of seafood and high animal protein in the human diet. Genome editing offers new possibilities such as the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology, which has the potential to accelerate the sustainable genetic improvement in fisheries and aquaculture. The CRISPR/Cas9 system has four key components, namely, target DNA, Cas9, the protospacer adjacent motif sequence, and the guide RNA or single-guide RNA. CRISPR/Cas is cheaper, easier, and more precise than the other genome editing technologies and can be used as a new breeding technology in fisheries and aquaculture to solve the far-reaching challenges. The attributes like high fecundity, external fertilization, short generation interval, the established method of breeding, and the larval rearing of most aquaculture species have advantages for CRISPR/Cas9 genome editing applications. CRISPR/Cas9 has recently been applied to the traits valued in some aquaculture species (almost >20 species), targeting the main traits of traditional genetic improvement initiatives like growth, disease resistance, reproduction, sterility, and pigmentation. Genome editing can fast forward the breeding process with precision where changes occur in the targeted genes. The probability of desired changes occurring and passing the trait in the next generation is high, so it takes 1-3 generations to establish a breed. Moreover, CRISPR/Cas genome editing rapidly introduces favorable changes by disrupting genes with targeted minor changes, in contrast to transgenesis, which introduces foreign genes into the host genome and thereby alleviates major public concerns on safety. Although the CRISPR/Cas technology has a tremendous potential, there are several technical challenges and regulatory and public issues concerning the applications in fisheries and the aquaculture breeding sector. Nonetheless, the exciting point in the CRISPR/Cas9 genome editing is that two CRISPR-edited fish, namely, red sea bream and tiger puffer developed by the Kyoto-based startup got approval and are now on the market for sale, and another fish, FLT-01 Nile tilapia developed by the AquaBounty, is not classified under genetically modified organism regulatory. However, there is still a way to go before it revolutionizes and becomes viable in commercial aquaculture as the new breeding technology for aquaculture-important traits and species.

show abstract

Genome Editing Using the CRISPR-Cas9 System to Generate a Solid-Red Germline of Nile Tilapia (Oreochromis niloticus)

Cited by 31 publications

References 94 publications

Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine

Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine

CRISPR Genome Editing Technology to Revolutionize Aquaculture and Fisheries

CRISPR/Cas Genome Editing—Can It Become a Game Changer in Future Fisheries Sector?

Contact Info

Product

Resources

About