Genome Editing with Cas9 in Lactobacilli

Vento, Justin M.; Beisel, Chase L.

doi:10.1007/978-1-0716-2233-9_16

Cited by 4 publications

(4 citation statements)

References 32 publications

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“…The number of people that are being suppressed is smaller. (24) Austin Burt described a sophisticated method using gene driving forces behind genes that only result in death or sterility when both versions are destroyed. When present, these 'genetic load' causes would spread quickly among slightly handicapped heterozygotes and ultimately contribute to a demographic collapse or perhaps extinction as a result of the accumulating burden of regressive genes.…”

Section: Engineered Gene Drivesmentioning

confidence: 99%

“…Surprisingly, a single guide RIBONUCLEIC ACID (sgRIBONUCLEIC ACID) of less than 100 base pairings in sequence can instruct CRISPR to cut a particular protospacer in the genome. (24) This instruction RIBONUCLEIC ACID must start with a spacers segment made up of 17-20 base pairs that are equal to the desired gene. Selecting protospacers inside the desired gene, creating one or more guide RIBONUCLEIC ACIDs with compatible separators, and sending CRISPR, those guide RIBONUCLEIC ACIDs, and altered repaired templates devoid of those protospacers into a cell are the steps in the procedure for editing (Figure 3).…”

Section: Genome Editing Using Ribonucleic Acid Guidance Using the Cri...mentioning

confidence: 99%

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The Possibilities of Gene Drives for Managing Populations and Controlling Diseases

Upadhye

Shah

Mudhol

2023

Salud, Ciencia y Tecnología

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Las limitaciones técnicas y el uso de impulsores genéticos para abordar problemas ecológicos modificando todas las poblaciones de especies salvajes siguen siendo principalmente especulativos. Aquí examinamos la posibilidad de que los impulsores genéticos guiados por ARN y basados en la nucleasa CRISPR Cas9 puedan utilizarse como un enfoque global para introducir características modificadas en poblaciones naturales durante un largo periodo. Esbozamos las capacidades potenciales y las posibles desventajas y ofrecemos nuevas medidas preventivas para detener la propagación de genes y deshacer las modificaciones genéticas. La edición de la población animal sexual beneficiaría notablemente tanto a las personas como al medio ambiente. Por ejemplo, los impulsores genéticos guiados por ARN podrían detener la propagación de enfermedades, ayudar a la agricultura corrigiendo los bichos y malas hierbas químicos y la resistencia a los herbicidas, y gestionar especies invasoras dañinas. Sin embargo, cada uso prospectivo debe evaluarse cuidadosamente debido a la probabilidad de repercusiones ecológicas desfavorables y a la casi inevitabilidad de la diseminación más allá de las fronteras políticas. Para investigar la aplicación responsable de esta tecnología ahora hipotética, queremos conversaciones públicas serias, inclusivas y educadas.

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Section: Engineered Gene Drivesmentioning

confidence: 99%

Section: Genome Editing Using Ribonucleic Acid Guidance Using the Cri...mentioning

confidence: 99%

The Possibilities of Gene Drives for Managing Populations and Controlling Diseases

Upadhye

Shah

Mudhol

2023

Salud, Ciencia y Tecnología

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“…Vento et al utilized two E. coli - Lactobacilli shuttle vectors: one containing SpCas9, tracrRNA, and single spacer CRISPR array; the other containing a dsDNA recombinant engineering template [ 69 ]. L. plantarum was transformed with an E. coli - Lactobacillus shuttle vector carrying a recombineering template (RT) and homologous arm (HA), and L. plantarum was edited using this technique.…”

Section: Development Of Crispr/cas9-based Genome Editing In ...mentioning

confidence: 99%

Development and Applications of CRISPR/Cas9-Based Genome Editing in Lactobacillus

Zhang

et al. 2022

IJMS

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Lactobacillus, a genus of lactic acid bacteria, plays a crucial function in food production preservation, and probiotics. It is particularly important to develop new Lactobacillus strains with superior performance by gene editing. Currently, the identification of its functional genes and the mining of excellent functional genes mainly rely on the traditional gene homologous recombination technology. CRISPR/Cas9-based genome editing is a rapidly developing technology in recent years. It has been widely applied in mammalian cells, plants, yeast, and other eukaryotes, but less in prokaryotes, especially Lactobacillus. Compared with the traditional strain improvement methods, CRISPR/Cas9-based genome editing can greatly improve the accuracy of Lactobacillus target sites and achieve traceless genome modification. The strains obtained by this technology may even be more efficient than the traditional random mutation methods. This review examines the application and current issues of CRISPR/Cas9-based genome editing in Lactobacillus, as well as the development trend of CRISPR/Cas9-based genome editing in Lactobacillus. In addition, the fundamental mechanisms of CRISPR/Cas9-based genome editing are also presented and summarized.

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“…However, dsDNA recombineering assisted by CRISPR/Cas9 was less effective in producing point mutations than ssDNA recombineering. Vento et al used two Escherichia coli-Lactobacilli shuttle vectors to perform genome editing in L. plantarum based on CRISPR/Cas9-mediated dsDNA recombineering (Vento and Beisel 2022). One vector expressed SpCas9, a single spacer CRISPR array, and tracrRNA, and the other vector contained a dsDNA editing template and homologous arm.…”

Section: Crispr/cas9-assisted Dsdna Recombineeringmentioning

confidence: 99%

CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products

Dong

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Lactic acid bacteria (LAB) plays a crucial role in aquatic products biopreservation as it can inhibit many bacteria, in particular the specific spoilage organisms (SSOs) of aquatic products, by competing for nutrients or producing one or more metabolites which have antimicrobial activity, such as bacteriocins. Lactobacillus spp. and Lactococcus spp. are the most commonly used Lactic acid bacterias in aquatic products preservation. The improvement of gene editing tools is particularly important for developing new lactic acid bacteria strains with superior properties for aquatic products biopreservation. This review summarized the research progress of the most widely used CRISPR/Cas-based genome editing tools in Lactobacillus spp. and Lactococcus spp. The genome editing tools based on homologous recombination and base editor were described. Then, the research status of CRISPRi in transcriptional regulation was reviewed briefly. This review may provide a reference for the application of CRISPR/Cas-based genome editing tools to other lactic acid bacteria species.

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Genome Editing with Cas9 in Lactobacilli

Cited by 4 publications

References 32 publications

The Possibilities of Gene Drives for Managing Populations and Controlling Diseases

The Possibilities of Gene Drives for Managing Populations and Controlling Diseases

Development and Applications of CRISPR/Cas9-Based Genome Editing in Lactobacillus

CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products

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