Genome engineering of Kluyveromyces marxianus for high D-( −)-lactic acid production under low pH conditions

Gosalawit, Chotika; Khunnonkwao, Panwana; Jantama, Kaemwich

doi:10.1007/s00253-023-12658-2

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…In the search of an alternative yeast to produce LA, the Crabtree-negative and thermo-tolerant Kluyveromyces marxianus was engineered to make D-lactate at a lower pH without compromising its growth [ 62 ]. The sole change in the genotype was the disruption of the single Pdc gene ( PDC1 ) by the introduction of a codon-optimized bacterial D-LDH ( ldhA ).…”

Section: Common Metabolic Engineering Strategiesmentioning

confidence: 99%

“…To prove its performance in a setting closer to industry, the authors showed that using the strain on sugarcane molasses, a low-value carbon source, it reached a titer 66.26 ± 0.81 g/L and a productivity of 0.91 ± 0.01 g/g without any additional nutrients. This study demonstrated that some yeasts may be able to reach yields of interest for industrial applications with much fewer changes in their genotype than S. cerevisiae [ 62 ].…”

Section: Common Metabolic Engineering Strategiesmentioning

confidence: 99%

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Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production

Melo,

de Oliveira Junqueira,

Lima

et al. 2024

JoF

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Lactic acid (LA) production has seen significant progress over the past ten years. LA has seen increased economic importance due to its broadening use in different sectors such as the food, medicine, polymer, cosmetic, and pharmaceutical industries. LA production bioprocesses using microorganisms are economically viable compared to chemical synthesis and can benefit from metabolic engineering for improved productivity, purity, and yield. Strategies to optimize LA productivity in microorganisms on the strain improvement end include modifying metabolic routes, adding gene coding for lactate transporters, inducing tolerance to organic acids, and choosing cheaper carbon sources as fuel. Many of the recent advances in this regard have involved the metabolic engineering of yeasts and filamentous fungi to produce LA due to their versatility in fuel choice and tolerance of industrial-scale culture conditions such as pH and temperature. This review aims to compile and discuss metabolic engineering innovations in LA production in yeasts and filamentous fungi over the 2013–2023 period, and present future directions of research in this area, thus bringing researchers in the field up to date with recent advances.

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Section: Common Metabolic Engineering Strategiesmentioning

confidence: 99%

Section: Common Metabolic Engineering Strategiesmentioning

confidence: 99%

Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production

Melo,

de Oliveira Junqueira,

Lima

et al. 2024

JoF

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Research progress on the biosynthesis of d-lactic acid from low-value biomass materials

Zu,

Wu,

Liao

et al. 2024

Biomass and Bioenergy

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Simultaneous saccharification and fermentation for d-lactic acid production using a metabolically engineered Escherichia coli adapted to high temperature

Pérez-Morales,

Caspeta,

Merino

et al. 2024

Biotechnol Biofuels

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Background Escherichia coli JU15 is a metabolically engineered strain capable to metabolize C5 and C6 sugars with a high yield of d-lactic acid production at its optimal growth temperature (37 °C). The simultaneous saccharification and fermentation process allow to use lignocellulosic biomass as a cost-effective and high-yield strategy. However, this process requires microorganisms capable of growth at a temperature close to 50 °C, at which the activity of cellulolytic enzymes works efficiently. Results The thermotolerant strain GT48 was generated by adaptive laboratory evolution in batch and chemostat cultures under temperature increments until 48 °C. The strain GT48 was able to grow and ferment glucose to d-lactate at 47 °C. It was found that a pH of 6.3 conciliated with GT48 growth and cellulase activity of a commercial cocktail. Hence, this pH was used for the SSF of a diluted acid-pretreated corn stover (DAPCS) at a solid load of 15% (w/w), 15 FPU/g-DAPCS, and 47 °C. Under such conditions, the strain GT48 exhibited remarkable performance, producing d-lactate at a level of 1.41, 1.42, and 1.48-fold higher in titer, productivity, and yield, respectively, compared to parental strain at 45 °C. Conclusions In general, our results show for the first time that a thermal-adapted strain of E. coli is capable of being used in the simultaneous saccharification and fermentation process without pre-saccharification stage at high temperatures.

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Genome engineering of Kluyveromyces marxianus for high D-( −)-lactic acid production under low pH conditions

Cited by 4 publications

References 37 publications

Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production

Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production

Research progress on the biosynthesis of d-lactic acid from low-value biomass materials

Simultaneous saccharification and fermentation for d-lactic acid production using a metabolically engineered Escherichia coli adapted to high temperature

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