Genome evolution driven by host adaptations results in a more virulent and antimicrobial-resistant Streptococcus pneumoniae serotype 14

Ding, Feng; Tang, Petrus; Hsu, Mei‐Hua; Cui, Peng; Hu, Songnian; Yu, Jun; Chiu, Cheng-Hsun

doi:10.1186/1471-2164-10-158

Cited by 61 publications

(53 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both insertion site and orientation were confirmed to be the same in the other four isolates sharing Tn6058. A large portion (almost 16 kb) of Tn6058, formed largely by cargo genes and including the pC194 plasmid, displayed high identity (97.3%) to a corresponding sequence of the Tn5253-related transposon Tn2008 (11) found in the sequenced genome of S. pneumoniae CGSP14, a virulent and multidrug-resistant invasive strain (accession no. CP001033).…”

Section: All Three Mef(e)-positive Isolates Harbored Tn2009 (10)mentioning

confidence: 99%

See 1 more Smart Citation

Heterogeneity of Tn 5253 -Like Composite Elements in Clinical Streptococcus pneumoniae Isolates

Mingoia¹,

Tili²,

Manso³

et al. 2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Several drug resistances in Streptococcus pneumoniae are associated with mobile genetic elements, which are loosely subdivided into a group of smaller (18-to 27-kb) and a group of larger (>50-kb) elements. While the elements of the former group, which typically carry the tetracycline resistance determinant tet(M) and whose prototype is Tn916 (18 kb), have been studied extensively, the larger elements, whose prototype is Tn5253 (ϳ65.5 kb), are not as well explored. Tn5253 is a composite structure consisting of two independent conjugative transposons, Tn5251 (which is virtually identical to Tn916) and Tn5252 (ϳ47.5 kb), with the former inserted into the latter. Tn5252, which so far has only partially been sequenced, carries an integrase gene, driving its site-specific insertion into the host cell genome, and the chloramphenicol resistance cat pC194 determinant. This study investigated 20 clinical isolates of S. pneumoniae, which were selected on the basis of cat pC194 -mediated chloramphenicol resistance. All 20 isolates harbored a Tn5253-like element. The composite elements (some of which have been completely sequenced) demonstrated considerable heterogeneity that stemmed from a dual variability: in the Tn5252-like element, due primarily to differences in the integrase gene but also to differences in cargo genes and in the overall genetic organization, and in the Tn916-like element, with the possible involvement, besides Tn916, of a number of Tn916 family pneumococcal elements carrying different erythromycin resistance genes. In mating experiments, only one composite element, containing a less typical Tn916 family element, appeared to be nonmobile. Being part of a Tn5253-like composite element may confer on some Tn916-like transposons, which are apparently nontransferable as independent genetic elements, the ability to be mobilized.A unique recombination-mediated genetic plasticity is a distinctive feature of Streptococcus pneumoniae (3) and a key to its success as a pathogen. In this context, a cause for serious concern is the emergence and increasing spread of multidrugresistant clinical pneumococci (28), where multiple resistance is generally associated with mobile genetic elements. Although the nomenclature for such elements is evolving, they are often referred to as transposons or as integrative and conjugative elements (ICEs) (21, 33). Pneumococcal mobile elements (which are also found in other streptococci and Gram-positive cocci) can be loosely subdivided into two major groups of smaller (18-to 27-kb) and larger (Ͼ50-kb) elements (2, 23).The smaller genetic elements form the widespread Tn916 family, so called from the prototype, Tn916, an 18-kb transposon originally detected in Enterococcus faecalis when it was still regarded as a Streptococcus species (12). Tn916 family elements typically contain integrase (int 916 ) and excisionase (xis 916 ) genes and carry the tetracycline (TET) resistance determinant tet(M). These elements have been widely investigated, leading to the discovery of a variety of Tn916 ...

show abstract

Section: All Three Mef(e)-positive Isolates Harbored Tn2009 (10)mentioning

confidence: 99%

“…FM211187) (8) and Tn2008 (ϳ67 kb; accession no. CP001033) (11), both carrying cat pC194 in their Tn5252-like transposon and sharing an identical genome integration site that is different from that of Tn5253.…”

mentioning

confidence: 99%

Heterogeneity of Tn 5253 -Like Composite Elements in Clinical Streptococcus pneumoniae Isolates

Mingoia¹,

Tili²,

Manso³

et al. 2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…In recent years, considerable progress has been made in the understanding of those large genetic elements, a distinct family of ICEs, whose prototype is Tn5253, a conjugative composite structure resulting from the insertion of Tn5251 (virtually identical to Tn916) into Tn5252 (10). The complete sequence of Tn5253 has become available and has lately been analyzed (11), and several studies have recently stressed the heterogeneity of Tn5253-like composite elements and their integrase genes (int 5252 or int Sp23FST81 ) (12)(13)(14)(15)(16)(17).…”

mentioning

confidence: 99%

Tn 5253 Family Integrative and Conjugative Elements Carrying mef (I) and catQ Determinants in Streptococcus pneumoniae and Streptococcus pyogenes

Mingoia

Morici

Morroni

et al. 2014

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The linkage between the macrolide efflux gene mef(I) and the chloramphenicol inactivation gene catQ was first described in Streptococcus pneumoniae (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in Streptococcus pyogenes (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253 family integrative and conjugative elements (ICEs). The ICE from S. pneumoniae (ICESpn529IQ) was sequenced, whereas the ICEs from S. pyogenes (ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share the int Sp23FST81 integrase gene and an identical Tn916 fragment, whereas ICESpy005IQ has int 5252 and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (int Sp23FST81 or int 5252 ). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation, mef(I) and catQ were cotransferred at a high frequency from S. pyogenes Spy005 and at very low frequencies from S. pneumoniae Spn529 and S. pyogenes Spy029.

show abstract

“…This is underscored by the observation that the heritability factor for dying of infectious disease is greater than any other condition including cardiovascular disease and cancer (Sorensen et al, 1988). Heightened risk is associated with rare defects in host immune defenses (Ram et al, 2010) or bacterial adaptation to clinical interventions (Ding et al, 2009). The pneumococcus is particularly adept at rapidly acquiring antibiotic resistance and evading capsule-based vaccines (Adamkiewicz et al, 2003; Halasa et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Genomic Analyses of Pneumococci from Children with Sickle Cell Disease Expose Host-Specific Bacterial Adaptations and Deficits in Current Interventions

Carter

Wolf

Opijnen

et al. 2014

Cell Host & Microbe

View full text Add to dashboard Cite

Summary Sickle cell disease (SCD) patients are at high risk of contracting pneumococcal infection. To address this risk, they receive pneumococcal vaccines, and antibiotic prophylaxis and treatment. To assess the impact of SCD and these interventions on pneumococcal genetic architecture, we examined the genomes of over 300 pneumococcal isolates from SCD patients over 20 years. Modern SCD strains retained invasive capacity but shifted away from the serotypes used in vaccines. These strains had specific genetic changes related to antibiotic resistance, capsule biosynthesis, metabolism and metal transport. A murine SCD model coupled with Tn-seq mutagenesis identified 60 non-capsular pneumococcal genes under differential selective pressure in SCD, which correlated with aspects of SCD pathophysiology. Further, virulence determinants in the SCD context were distinct from the general population and protective capacity of potential antigens was lost over time in SCD. This highlights the importance of understanding bacterial pathogenesis in the context of high-risk individuals.

show abstract

Genome evolution driven by host adaptations results in a more virulent and antimicrobial-resistant Streptococcus pneumoniae serotype 14

Cited by 61 publications

References 56 publications

Heterogeneity of Tn 5253 -Like Composite Elements in Clinical Streptococcus pneumoniae Isolates

Heterogeneity of Tn 5253 -Like Composite Elements in Clinical Streptococcus pneumoniae Isolates

Tn 5253 Family Integrative and Conjugative Elements Carrying mef (I) and catQ Determinants in Streptococcus pneumoniae and Streptococcus pyogenes

Genomic Analyses of Pneumococci from Children with Sickle Cell Disease Expose Host-Specific Bacterial Adaptations and Deficits in Current Interventions

Contact Info

Product

Resources

About