Genome Mapping and Map Based Cloning

Kole, C.; Gupta, P. K.

doi:10.1007/978-94-007-1040-5_11

Cited by 8 publications

(5 citation statements)

References 225 publications

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“…Traditionally, geneticists relied on map-based cloning for the identification of mutant genes, a method involving the labor-intensive process of associating markers with the mutant phenotype and precisely mapping the candidate genes. However, this approach requires large populations and an extensive array of markers, making it time-consuming ( Kole and Gupta, 2004 ). The emergence of high-throughput sequencing has revolutionized mutant gene identification in plants, offering several methods summarized here ( Figure 2 ) and their corresponding applications ( Supplementary Table S2 ).…”

Section: High-throughput Sequencing For Mutant Gene Identificationmentioning

confidence: 99%

Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding

Wang,

Abbas,

et al. 2024

Front. Plant Sci.

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Cotton production faces challenges in fluctuating environmental conditions due to limited genetic variation in cultivated cotton species. To enhance the genetic diversity crucial for this primary fiber crop, it is essential to augment current germplasm resources. High-throughput sequencing has significantly impacted cotton functional genomics, enabling the creation of diverse mutant libraries and the identification of mutant functional genes and new germplasm resources. Artificial mutation, established through physical or chemical methods, stands as a highly efficient strategy to enrich cotton germplasm resources, yielding stable and high-quality raw materials. In this paper, we discuss the good foundation laid by high-throughput sequencing of cotton genome for mutant identification and functional genome, and focus on the construction methods of mutant libraries and diverse sequencing strategies based on mutants. In addition, the important functional genes identified by the cotton mutant library have greatly enriched the germplasm resources and promoted the development of functional genomes. Finally, an innovative strategy for constructing a cotton CRISPR mutant library was proposed, and the possibility of high-throughput screening of cotton mutants based on a UAV phenotyping platform was discussed. The aim of this review was to expand cotton germplasm resources, mine functional genes, and develop adaptable materials in a variety of complex environments.

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Section: High-throughput Sequencing For Mutant Gene Identificationmentioning

confidence: 99%

Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding

Wang,

Abbas,

et al. 2024

Front. Plant Sci.

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“…A reliable and successful method is the markerassisted selection (MAS). Both Collard and Mackill [70] and Kole and Gupta [71] have shown the benefits of MAS over traditional phenotypic selection. Compared to phenotypic breeding, selection utilizing molecular markers is easier.…”

Section: Necessity Of Molecular Breedingmentioning

confidence: 99%

Capsicum: Breeding Prospects and Perspectives for Higher Productivity

Selvakumar¹,

Manjunathagowda²,

Singh³

2023

Capsicum - Current Trends and Perspectives

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Chipotle peppers were grown in America before being carried to Europe by Columbus. Capsicum breeding began with choosing wild species for desired characteristics, with additional development based on precision selection. To improve capsicum yields, traditional methods such as mass selection, pedigree, single-seed descent, backcrossing, and hybridization are being used. Capsicum has a high level of genetic diversity due to multiple new gene rearrangements. Capsicum fruits are high in nutrients that are beneficial to human health. As a result, the world market for and consumption of capsicum has lately grown. Capsicum breeding programmes aim to improve yield, biotic, abiotic resistance, and nutritional quality. Recent breakthroughs in capsicum breeding have included introgression, mutation breeding, polyploidy, haploidy, embryo rescue, and the use of genetic markers. Molecular technology has grown into an important tool that, when coupled with classic selection and hybridization procedures, has the potential to result in great success in an established capsicum genetic breeding programme.

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“…Genetic mapping using molecular markers has been used as potential for mapping, monitoring and cloning of oligogenes of agronomic importance (Kole and Gupta 2001). This technique has also facilitated detection ⎯⎯⎯⎯ of quantitative trait loci (Landjeva et al 2008) and their Mendelization leading to precise genetic dissection and manipulation as tacitly as oligogenes .…”

Section: Introductionmentioning

confidence: 99%

“…Comparative mapping has emerged as a useful strategy in molecular genetics to decipher homologous genes and gene clusters of economic interests across taxa (Kole and Gupta 2001). A homologous gene can be cloned and used for genetic tailoring of a number of species through genetic transformation.…”

Section: Introductionmentioning

confidence: 99%

RFLP mapping of loci controlling self-incompatibility in Brassica campestris and their comparative mapping with B. napus and B. oleracea

Panigrahi¹,

Kole²,

Kole³

2011

Biologia plant.

Self Cite

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RFLP analysis of a cDNA probe SLG6, governing self incompatibility (SI) in Brassica oleracea, using a recombinant inbred population of Brassica campestris followed by genetic linkage analysis led to the detection of two marker loci, SLG6a and SLG6b controlling SI. SLG6a was mapped in linkage group (LG) 9 and was flanked by the RFLP markers ec4f10 (6.4 cM) and wg5b9 (4.2 cM). SLG6b positioned in LG 2 and was flanked by the RFLP markers wg2d11 (9.9 cM) and ec4e7 (26.9 cM). These results indicated the scope of marker-aided introgression of these genes into selfcompatible genotypes for production of SI lines suitable for hybridization in B. campestris. Comparative mapping of LG 9 containing SLG6b with corresponding linkage groups of B. oleracea (BO 2) and B. napus (BN 16) led to the detection of small homologous regions with SLG6 locus linked with another RFLP locus. This evidenced for homology of the SLG genes across Brassica species and possibility of using any single cloned SLG gene for development of SI lines in any Brassica species.

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Genome Mapping and Map Based Cloning

Cited by 8 publications

References 225 publications

Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding

Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding

Capsicum: Breeding Prospects and Perspectives for Higher Productivity

RFLP mapping of loci controlling self-incompatibility in Brassica campestris and their comparative mapping with B. napus and B. oleracea

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