Genome Multiplicity and Radiation Resistance in Micrococcus radiodurans1

Harsojo,; Kitayama, Shigeru; Matsuyama, Akira

doi:10.1093/oxfordjournals.jbchem.a133544

Cited by 75 publications

(40 citation statements)

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“…Under UV radiation, the DEIRA chromosomes generally fracture, but in less than 3 h, the fragments can be reassembled without loss of viability. But, Harsojo et al (11) reported that, for varied genome complements in DEIRA, there is no change in radiation resistance capacities. Actually, E. coli and Vibrio cholerae during fast growth contain at least three genome equivalents, and it is suggested that the extra chromosomal equivalents facilitate interchromosomal recombination and repair (3).…”

Section: Deira Resistance To Ionizing Radiation and Uv Damagementioning

confidence: 99%

Predicted highly expressed and putative alien genes of Deinococcus radiodurans and implications for resistance to ionizing radiation damage

Karlin

Mrázek

2001

Proc. Natl. Acad. Sci. U.S.A.

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Predicted highly expressed (PHX) and putative alien genes determined by codon usages are characterized in the genome of Deinococcus radiodurans (strain R1). Deinococcus radiodurans (DEIRA) can survive very high doses of ionizing radiation that are lethal to virtually all other organisms. It has been argued that DEIRA is endowed with enhanced repair systems that provide protection and stability. However, predicted expression levels of DNA repair proteins with the exception of RecA tend to be low and do not distinguish DEIRA from other prokaryotes. In this paper, the capability of DEIRA to resist extreme doses of ionizing and UV radiation is attributed to an unusually high number of PHX chaperone͞degradation, protease, and detoxification genes. Explicitly, compared with all current complete prokaryotic genomes, DEIRA contains the greatest number of PHX detoxification and protease proteins. Other sources of environmental protection against severe conditions of UV radiation, desiccation, and thermal effects for DEIRA are the several S-layer (surface structure) PHX proteins. The top PHX gene of DEIRA is the multifunctional tricarboxylic acid (TCA) gene aconitase, which, apart from its role in respiration, also alerts the cell to oxidative damage.

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Section: Deira Resistance To Ionizing Radiation and Uv Damagementioning

confidence: 99%

Predicted highly expressed and putative alien genes of Deinococcus radiodurans and implications for resistance to ionizing radiation damage

Karlin

Mrázek

2001

Proc. Natl. Acad. Sci. U.S.A.

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“…The former isolate was obtained from A.W. Anderson (Oregon Agricultural Experiment Station) in the 1960s, and the latter was obtained from B. E. B. Moseley (Edinburgh University) in the early 1980s (Harsojo et al, 1981). We speculate that repeated transposition events had occurred during the subcultivation of strain MR 1 in Moseley's laboratory.…”

Section: Resultsmentioning

confidence: 94%

“…They are different from each other in several respects including the growth in minimal medium (Harsojo et al, 1981), the sensitivity to mitomycin-C (Kitayama et al, 1983) and the plasmid profile (Kikuchi et al, 1994). To facilitate biochemical and genetic experiments using D. radiodurans , Kitayama and Matsuyama generated strain KD8301 by transforming a DNase-deficient and adenine auxotroph of strain KR 1 with DNA from a streptomycin-resistant strain of KR 1 (Kitayama and Matsuyama, 1975).…”

Section: Introductionmentioning

confidence: 99%

Characterization and distribution of IS8301 in the radioresistant bacterium Deinococcus radiodurans.

et al. 2003

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The insertion sequence element IS 8301 isolated from the radiation resistant bacterium Deinococcus radiodurans strain KD8301 was characterized. IS 8301 is comprised of 1,736-bp, lacks terminal inverted repeats and does not duplicate target DNA upon its insertion. The amino acid sequence homology of two open reading frames encoded in IS 8301 indicates that this insertion sequence element belongs to the IS 200 /IS 605 group. There were seven loci completely identical with the IS 8301 sequence in the published D. radiodurans R 1 genome sequence. The genome distribution profiles of IS 8301 in strain KD8301 as well as in the three different laboratory isolates (KR 1 , MR 1 , and R 1 ) of wild-type D. radiodurans were investigated using genomic hybridization analysis. At least 21 strong hybridization signals were detected in strain KD8301 while only one hybridization signal was detected in strain KR 1 , the parent strain of KD8301. In strain MR 1 , a different wild-type isolate, six strong hybridization signals were detected. In spite of the identification of seven copies of IS 8301 in the published D. radiodurans R 1 genome sequence, only one hybridization signal was detected in strain R 1 purchased from American Type Culture Collection. Using inverse PCR and sequencing analyses, total 13 different insertion loci of IS 8301 in the D. radiodurans genome were identified. Sequence comparison of the flanking region of insertion sites indicated that the sequence 5'-TTGAT-3' preceded the left end of IS 8301 in all cases.

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“…In interstrand cross-links, duplex DNA is still linked together even if two nicks are introduced at the 5?-side of the lesion. In this bacterium presence of multiple copies of genome in a cell is known, 18) so the damaged DNA can be repaired among the copies by genetic recombination. In contrast, UV-endonuclease a (a homologue of UvrA) can introduce four incisions, two each at the 5?-and 3?-sides of duplex DNA, and then the cross-linked DNA fragment can be removed.…”

mentioning

confidence: 99%