Genome of Serratia plymuthica UBCF_13, Insight into diverse unique traits

Abstract

Background: Whole genome sequencing is become an essential tool to explore potential of microorganism and evolutionary study. The Serratia plymuthica UBCF_13 is one of phylloplane associated plant bacteria showing antifungal activity. For that reason, its complete genome information is necessary to enhance its potential as biocontrol against plant pathogenic fungal. Here, we report the genome sequence of Serratia plymuthica UBCF_13 to understand the molecular mechanism regarding its biocontrol ability. Methods: Continuous short reads were attained from Illumina sequencing runs and reads 150 bp were merged into a single dataset. Pan-genome based method was used to identify core-genome of S. plymuthica species and unique gene in UBCF_13. Results: Assambled Illumina reads of S. plymuthica strain UBCF_13 genome was produced a 5.46 Mb circular genome sequence. It was found 3321 genes belong to the core-genome sheared by the 18 strains evaluated. The UBCF_13 genome harbor 485 unique genes, where 300 of them only can be found in this strain Conclusions: The sequence of UBCF_13 genome sequence data will contribute for further exploration of the potential of S. plymuthica UBCF_13 as bacteria producing antibiotic.

Growth Phase Influence the Gene Expression and Metabolite Production Related to Indole-3-Acetic Acid (IAA) Biosynthesis by Serratia plymuthica UBCF_13

“…Genome mining and qRT-PCR of genes related to IAA biosynthesis in UBCF̲13: Genome mining of genes that are related to IAA biosynthesis from the genome of S. plymuthica UBCF̲13 (RefSeq: NZ̲CP068771.1) 19 refers to Batista et al 20 , with modifications. The predicted genes were confirmed using blastx (NCBI) to get the putative function and BlastKoala (https://www.kegg.jp/blastkoala/) to find the KEGG Orthology (KO) number.…”