Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes

Wu, Xingjie; Rensing, Christopher; Han, Dongfei; Xiao, Ke‐Qing; Dai, Yuexiu; Tang, Zhen‐Xing; Liesack, Werner; Peng, Jingjing; Cui, Zhenling; Zhang, Fusuo

doi:10.1128/msystems.01107-21

Cited by 112 publications

(53 citation statements)

References 64 publications

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“…Therefore, the phylum Bacteriodota contributes to mineral phosphate solubilization as well as the family Cyclobacteriaceae [ 46 ]. The bacterial species of the phylum Acidobacteriota have genes that probably help in survival and competitive colonization of the rhizosphere, leading to the establishment of beneficial relationships with plants, regulation of biogeochemical cycles, decomposition of biopolymers, exopolysaccharide secretion, and plant growth promotion [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Microbial Profiling of Potato-Associated Rhizosphere Bacteria under Bacteriophage Therapy

et al. 2022

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Potato soft rot and wilt are economically problematic diseases due to the lack of effective bactericides. Bacteriophages have been studied as a novel and environment-friendly alternative to control plant diseases. However, few experiments have been conducted to study the changes in plants and soil microbiomes after bacteriophage therapy. In this study, rhizosphere microbiomes were examined after potatoes were separately infected with three bacteria (Ralstonia solanacearum, Pectobacterium carotovorum, Pectobacterium atrosepticum) and subsequently treated with a single phage or a phage cocktail consisting of three phages each. Results showed that using the phage cocktails had better efficacy in reducing the disease incidence and disease symptoms’ levels when compared to the application of a single phage under greenhouse conditions. At the same time, the rhizosphere microbiota in the soil was affected by the changes in micro-organisms’ richness and counts. In conclusion, the explicit phage mixers have the potential to control plant pathogenic bacteria and cause changes in the rhizosphere bacteria, but not affect the beneficial rhizosphere microbes.

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Section: Discussionmentioning

confidence: 99%

Microbial Profiling of Potato-Associated Rhizosphere Bacteria under Bacteriophage Therapy

et al. 2022

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“…However, this diffusion-dependent uptake process is rather inefficient, which may be a disadvantage when only low amounts of PQQ are present As a "common good", PQQ like vitamin B12 may promote interspecies interactions in the microbiome (Sokolovskaya et al, 2020). PQQdependent gluconate production is the characteristic for numerous plant growth-promoting bacteria (Meyer et al, 2011;Shen et al, 2012;Wu et al, 2022). Organic acids like gluconate mobilise phosphate from insoluble calcium phosphates and thereby further bacterial and plant growth.…”

Section: Discussionmentioning

confidence: 99%

The TonB‐dependent uptake of pyrroloquinoline‐quinone (PQQ) and secretion of gluconate by Escherichia coli K‐12

Hantke

Friz

2022

Molecular Microbiology

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Enterobacteria like Escherichia coli use glucose as the preferred carbon source. The uptake depends on the phosphotransferase (PTS) system, which is required for translocation of the carbohydrate across the inner membrane concomitant with its phosphorylation. However, glucose may also be oxidised in the periplasm to gluconolactone by the glucose dehydrogenase Gcd (Van Schie et al., 1985). In addition, the periplasmic soluble aldose sugar dehydrogenase Asd (YliI), which has a very broad substrate specificity, may contribute to glucose oxidation, leading to formation of gluconate (Southall et al., 2006). Gluconate may then be transported into the cell by one of the four sugar acid transporters GntU, GntT, GntP, or IdnT (=GntW) (Peekhaus et al., 1997) for further catabolism.

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“…As a “common good”, PQQ like vitamin B12 may promote interspecies interactions in the microbiome (Sokolovskaya et al, 2020). PQQ dependent gluconate production is characteristic for numerous plant growth promoting bacteria (Shen et al, 2012, Meyer et al, 2011, Wu et al, 2022). Organic acids like gluconate mobilise phosphate from insoluble calcium phosphates and thereby further plant growth.…”

Section: Discussionmentioning

confidence: 99%

The TonB dependent uptake of pyrroloquinoline-quinone (PQQ) and secretion of gluconate by Escherichia coli K-12

Hantke

Friz

2022

Preprint

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Glucose is taken up by Escherichia coli through the phosphotransferase system (PTS) as the preferred carbon source. PTS mutants grow with glucose as a carbon source only in the presence of pyrroloquinoline quinone (PQQ), which is needed as a redox cofactor for the glucose dehydrogenase Gcd. The membrane-anchored Gcd enzyme oxidizes glucose to gluconolactone in the periplasm. For this reaction to occur, external supply of PQQ is required as E. coli is unable to produce PQQ de novo. Growth experiments show that PqqU (YncD) is the TonB-ExbBD dependent transporter for PQQ through the outer membrane. PQQ protected the cells from the PqqU dependent phage IsaakIselin (Bas10) by competition for the receptor protein. As a high affinity uptake system PqqU allows E. coli to activate Gcd even at surrounding PQQ concentrations of about 1 nmol/l. At about 30 fold higher PQQ concentrations the activation of Gcd gets PqqU independent. Due to its small size Pqq may also pass the outer membrane through porins. The PQQ dependent production of gluconate has been demonstrated in many plant growth promoting bacteria that solubilise phosphate minerals in the soil by secreting this acid. Under Pi limiting conditions also E. coli induces the glucose dehydrogenase and secretes gluconate, even in absence of PTS, that is, even when the bacterium is unable to grow on glucose without PQQ

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Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes

Cited by 112 publications

References 64 publications

Microbial Profiling of Potato-Associated Rhizosphere Bacteria under Bacteriophage Therapy

Microbial Profiling of Potato-Associated Rhizosphere Bacteria under Bacteriophage Therapy

The TonB‐dependent uptake of pyrroloquinoline‐quinone (PQQ) and secretion of gluconate by Escherichia coli K‐12

The TonB dependent uptake of pyrroloquinoline-quinone (PQQ) and secretion of gluconate by Escherichia coli K-12

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