Genome-scale CRISPR-Cas9 knockout screening in gastrointestinal stromal tumor with Imatinib resistance

Cao, Jie; Wei, Jianchang; Yang, Ping; Zhang, Tong; Chen, Zhuanpeng; He, Feng; Fang, Wei; Chen, Huacui; He, Hong; Zhong, Junbin; Yang, Zhi; Cai, Wei; Li, Wanglin; Qiang, Wang

doi:10.1186/s12943-018-0865-2

Cited by 37 publications

(33 citation statements)

References 9 publications

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“…This finding indicates that these putative protein–protein associations might participate in embryogenesis and tumorigenesis in C. japonica . Additionally, ACYP1 was found to be involved in gastrointestinal stromal tumor cells [82], and AAED1 was a potential target for gastric cancer [83]. SOX3 might also be related to tumorigenesis in either a negative or positive manner [84].…”

Section: Resultsmentioning

confidence: 99%

Systematic Identification and Evolution Analysis of Sox Genes in Coturnix japonica Based on Comparative Genomics

Lan

Ding³

et al. 2019

Genes

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Coturnix japonica (Japanese quail) has been extensively used as a model animal for biological studies. The Sox gene family, which was systematically characterized by a high-mobility group (HMG-box) in many animal species, encodes transcription factors that play central roles during multiple developmental processes. However, genome-wide investigations on the Sox gene family in birds are scarce. In the current study, we first performed a genome-wide study to explore the Sox gene family in galliform birds. Based on available genomic sequences retrieved from the NCBI database, we focused on the global identification of the Sox gene family in C. japonica and other species in Galliformes, and the evolutionary relationships of Sox genes. In our result, a total of 35 Sox genes in seven groups were identified in the C. japonica genome. Our results also revealed that dispersed gene duplications contributed the most to the expansion of the Sox gene family in Galliform birds. Evolutionary analyses indicated that Sox genes are an ancient gene family, and strong purifying selections played key roles in the evolution of CjSox genes of C. japonica. More interestingly, we observed that most Sox genes exhibited highly embryo-specific expression in both gonads. Our findings provided new insights into the molecular function and phylogeny of Sox gene family in birds.

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Section: Resultsmentioning

confidence: 99%

Systematic Identification and Evolution Analysis of Sox Genes in Coturnix japonica Based on Comparative Genomics

Lan

Ding³

et al. 2019

Genes

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“…It combines limitation of de novo fatty acid synthesis and the cholinergic anti-inflammatory pathway that confirms anticancer function [ 26 ]. Cao et al reported that ACYP1 was lower in imatinib-resistant gastrointestinal stromal tumor T1 cells [ 27 ]. Silencing POLR1A can hinder G1-S cell cycle progression in p53-inactivated human cancer cell lines [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and prognostic value of metabolism-related genes in gastric cancer

Fang

Huang

et al. 2020

Aging

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Gastric cancer (GC) is one of the most commonly occurring cancers, and metabolism-related genes (MRGs) are associated with its development. Transcriptome data and the relevant clinical data were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus databases, and we identified 194 MRGs differentially expressed between GC and adjacent nontumor tissues. Through univariate Cox and lasso regression analyses we identified 13 potential prognostic differentially expressed MRGs (PDEMRGs). These PDEMRGs (CKMT2, ME1, GSTA2, ASAH1, GGT5, RDH12, NNMT, POLR1A, ACYP1, GLA, OPLAH, DCK, and POLD3) were used to build a Cox regression risk model to predict the prognosis of GC patients. Further univariate and multivariate Cox regression analyses showed that this model could serve as an independent prognostic parameter. Gene Set Enrichment Analysis showed significant enrichment pathways that could potentially contribute to pathogenesis. This model also revealed the probability of genetic alterations of PDEMRGs. We have thus identified a valuable metabolic model for predicting the prognosis of GC patients. The PDEMRGs in this model reflect the dysregulated metabolic microenvironment of GC and provide useful noninvasive biomarkers.

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“…The results identified and verified that DCK and DCTPP1 are key genes that mediate NUC-1031 resistance [128]. Based on this screening method and theory, new drug resistance genes and anti-drug resistance targets were discovered [130,131]. The methods of gene editing and genome-scale CRISPR-Cas9 knockout screening provide a new direction for exploring drug resistance-related genes and drug-resistance mechanisms.…”

Section: Genome-scale Screening Drug Resistant Genesmentioning

confidence: 99%

Chemoresistance in Pancreatic Cancer

Zeng

Pöttler

Lan

et al. 2019

IJMS

441

306

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Pancreatic ductal adenocarcinoma (PDAC), generally known as pancreatic cancer (PC), ranks the fourth leading cause of cancer-related deaths in the western world. While the incidence of pancreatic cancer is displaying a rising tendency every year, the mortality rate has not decreased significantly because of late diagnosis, early metastasis, and limited reaction to chemotherapy or radiotherapy. Adjuvant chemotherapy after surgical resection is typically the preferred option to treat early pancreatic cancer. Although 5-fluorouracil/leucovorin with irinotecan and oxaliplatin (FOLFIRINOX) and gemcitabine/nab-paclitaxel can profoundly improve the prognosis of advanced pancreatic cancer, the development of chemoresistance still leads to poor clinical outcomes. Chemoresistance is multifactorial as a result of the interaction among pancreatic cancer cells, cancer stem cells, and the tumor microenvironment. Nevertheless, more pancreatic cancer patients will benefit from precision treatment and targeted drugs. Therefore, we outline new perspectives for enhancing the efficacy of gemcitabine after reviewing the related factors of gemcitabine metabolism, mechanism of action, and chemoresistance.

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Genome-scale CRISPR-Cas9 knockout screening in gastrointestinal stromal tumor with Imatinib resistance

Cited by 37 publications

References 9 publications

Systematic Identification and Evolution Analysis of Sox Genes in Coturnix japonica Based on Comparative Genomics

Systematic Identification and Evolution Analysis of Sox Genes in Coturnix japonica Based on Comparative Genomics

Identification and prognostic value of metabolism-related genes in gastric cancer

Chemoresistance in Pancreatic Cancer

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