Genome-Scale In Silico Analysis for Enhanced Production of Succinic Acid in Zymomonas mobilis

Widiastuti, Hanifah; Lee, Na-Rae; Karimi, Iftekhar A.; Lee, Dong-Yup

doi:10.3390/pr6040030

Cited by 11 publications

(6 citation statements)

References 57 publications

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“…The succinate yield is strongly related to nicotinamide adenine dinucleotide (NADH) availability [42] and, hence, to fumarate. Subsequently, a flux distribution that supports high fumarate and NADH presence is in favor of succinate production [43][44][45].…”

Section: Conversion Of Fa Into Succinic Acid In Actinobacillus Succinogenesmentioning

confidence: 99%

Well Knowledge of the Physiology of Actinobacillus succinogenes to Improve Succinic Acid Production

Salma

Djelal

Abdallah

et al. 2021

Applied Microbiology

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The anaerobic fermentation of glucose and fructose was performed by Actinobacillus succinogenes 130Z in batch mode using three different volume of bioreactors (0.25, 1 and 3 L). The strategy used was the addition of MgCO3 and fumaric acid (FA) as mineral carbon and the precursor of succinic acid, respectively, in the culture media. Kinetics and yields of succinic acid (SA) production in the presence of sugars in a relevant synthetic medium were investigated. Work on the bench scale (3 L) showed the best results when compared to the small anaerobic reactor's succinic acid yield and productivity after 96 h of fermentation. For an equal mixture of glucose and fructose used as substrate at 0.4 mol L−1 with the addition of FA as enhancer and under proven optimal conditions (pH 6.8, T = 37 °C, anaerobic condition and 1% v/v of biomass), about 0.5 mol L−1 of SA was obtained, while the theoretical production of succinic acid was 0.74 mol L−1. This concentration corresponded to an experimental yield of 0.88 (mol-C SA/mol-C sugars consumed anaerobically) and a volumetric productivity of 0.48 g-SA L−1 h−1. The succinic acid yield and concentration obtained were significant and in the order of those reported in the literature.

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Section: Conversion Of Fa Into Succinic Acid In Actinobacillus Succinogenesmentioning

confidence: 99%

Well Knowledge of the Physiology of Actinobacillus succinogenes to Improve Succinic Acid Production

Salma

Djelal

Abdallah

et al. 2021

Applied Microbiology

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“…Comprehensive carbon metabolism model (375 reactions) to analyze the metabolism and predict knockout strategies for maximum SA production with maintaining the cell growth 2018 [95] A. succinogenes Genome-scale metabolic model to evaluate the metabolic capability of the strain to produce SA under various conditions 2018 [30] Zymomonas mobilis Genome-scale metabolic model to characterize SA-producing capability and comparatively identify gene deletions for enhanced SA production 2018 [96] E. coli Optimization modeling to identify near-optimal knockout genes for the maximum production of SA 2020 [97] Aspergillus niger…”

Section: E Coli and A Succinogenesmentioning

confidence: 99%

Insights on the Advancements of In Silico Metabolic Studies of Succinic Acid Producing Microorganisms: A Review with Emphasis on Actinobacillus succinogenes

et al. 2021

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Succinic acid (SA) is one of the top candidate value-added chemicals that can be produced from biomass via microbial fermentation. A considerable number of cell factories have been proposed in the past two decades as native as well as non-native SA producers. Actinobacillus succinogenes is among the best and earliest known natural SA producers. However, its industrial application has not yet been realized due to various underlying challenges. Previous studies revealed that the optimization of environmental conditions alone could not entirely resolve these critical problems. On the other hand, microbial in silico metabolic modeling approaches have lately been the center of attention and have been applied for the efficient production of valuable commodities including SA. Then again, literature survey results indicated the absence of up-to-date reviews assessing this issue, specifically concerning SA production. Hence, this review was designed to discuss accomplishments and future perspectives of in silico studies on the metabolic capabilities of SA producers. Herein, research progress on SA and A. succinogenes, pathways involved in SA production, metabolic models of SA-producing microorganisms, and status, limitations and prospects on in silico studies of A. succinogenes were elaborated. All in all, this review is believed to provide insights to understand the current scenario and to develop efficient mathematical models for designing robust SA-producing microbial strains.

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“…The paper by Widiastuti et al [7] outlines a model-driven strategy to estimate and improve the production capability of microbes based on an in silico analysis of genome-scale metabolic networks. The strategy explores the use of Zymomonas mobilis to produce succinic acid.…”

Section: Network-based Biological Systems Analysis and Optimizationmentioning

confidence: 99%

Special Issue on “Biological Networks”

Bagheri

2018

Processes

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Genome-Scale In Silico Analysis for Enhanced Production of Succinic Acid in Zymomonas mobilis

Cited by 11 publications

References 57 publications

Well Knowledge of the Physiology of Actinobacillus succinogenes to Improve Succinic Acid Production

Well Knowledge of the Physiology of Actinobacillus succinogenes to Improve Succinic Acid Production

Insights on the Advancements of In Silico Metabolic Studies of Succinic Acid Producing Microorganisms: A Review with Emphasis on Actinobacillus succinogenes

Special Issue on “Biological Networks”

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