Genome-scale metabolic reconstruction for the insidious bacterium in aquaculture Piscirickettsia salmonis

Fuentealba, Pablo; Aros, Camila; Latorre, Yesenia; Martínez, Irene; Marshall, Sergio H.; Ferrer, Pau; Albiol, Joan; Altamirano, Claudia

doi:10.1016/j.biortech.2016.10.024

Cited by 12 publications

(14 citation statements)

References 42 publications

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“…As described in the previous section, strain LF-89 cultures on different defined media only showed growth in cases were glucose was added and unexpectedly showed no growth on media containing amino acids as sole carbon source, in contrast with results obtained by Fuentealba et al (2017) . These differences led us to perform additional growth experiments using different P. salmonis strains.…”

Section: Resultsmentioning

confidence: 52%

“…Through constraint-based analysis we identified key metabolites required for P. salmonis growth. As has been previously shown, P. salmonis is auxotroph for several amino acids, including cysteine ( Fuentealba et al, 2017 ). Our in silico analysis indicated that the requirement for cysteine could also be fulfill by glutathione, through the action of γ-glutamyl transpeptidase (GGT).…”

Section: Discussionmentioning

confidence: 74%

“…We compared iPS584 with model iPF215, the first metabolic model for P. salmonis LF-89 ( Fuentealba et al, 2017 ). iPF215 allowed to predict that P. salmonis is auxotrophic for several amino acids and has the ability to use some of these compounds as carbon and energy sources.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, the use of a higher concentration of glutamate, could not compensate the absence of sugar in the medium. However, in the work of Fuentealba et al (2017) growth was reported in a medium free of sugars where amino acids were used as sole carbon sources. This shows that gluconeogenesis is not necessary incomplete in P. salmonis LF-89 metabolic network.…”

Section: Resultsmentioning

confidence: 99%

“…Main goals of these efforts have been to find links between metabolism and pathogenesis, to predict drug targets and in some cases to identify nutrient requirements ( Chavali et al, 2008 ; Oberhardt et al, 2008 ). In the case of P. salmonis a first metabolic model for strain LF-89 was recently presented, accounting for 215 of the pathogen’s genes ( Fuentealba et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Piscirickettsia salmonis Metabolism Using Genome-Scale Reconstruction, Modeling, and Testing

et al. 2017

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Piscirickettsia salmonis is an intracellular bacterial fish pathogen that causes piscirickettsiosis, a disease with highly adverse impact in the Chilean salmon farming industry. The development of effective treatment and control methods for piscireckttsiosis is still a challenge. To meet it the number of studies on P. salmonis has grown in the last couple of years but many aspects of the pathogen’s biology are still poorly understood. Studies on its metabolism are scarce and only recently a metabolic model for reference strain LF-89 was developed. We present a new genome-scale model for P. salmonis LF-89 with more than twice as many genes as in the previous model and incorporating specific elements of the fish pathogen metabolism. Comparative analysis with models of different bacterial pathogens revealed a lower flexibility in P. salmonis metabolic network. Through constraint-based analysis, we determined essential metabolites required for its growth and showed that it can benefit from different carbon sources tested experimentally in new defined media. We also built an additional model for strain A1-15972, and together with an analysis of P. salmonis pangenome, we identified metabolic features that differentiate two main species clades. Both models constitute a knowledge-base for P. salmonis metabolism and can be used to guide the efficient culture of the pathogen and the identification of specific drug targets.

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Section: Resultsmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Piscirickettsia salmonis Metabolism Using Genome-Scale Reconstruction, Modeling, and Testing

et al. 2017

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Engineering a defined culture medium to grow Piscirickettsia salmonis for its use in vaccine formulations

Fuentealba

Latorre

González

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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Piscirickettsia salmonis is a facultative Gram-negative intracellular bacterium that produces piscirickettsiosis, disease that causes a high negative impact in salmonid cultures. The so-far-unidentified nutritional requirements have hindered its axenic culture at laboratory and industrial scales for the formulation of vaccines. The present study describes the development of a defined culture medium for P. salmonis. The culture medium was formulated through rational design involving auxotrophy test and statistical designs of experiments, considering the genome-scale metabolic reconstruction of P. salmonis reported by our group. The whole optimization process allowed for a twofold increase in biomass and a reduction of about 50% of the amino acids added to the culture medium. The final culture medium contains twelve amino acids, where glutamic acid, threonine and arginine were the main carbon and energy sources, supporting 1.65 g/L of biomass using 6.5 g/L of amino acids in the formulation. These results will contribute significantly to the development of new operational strategies to culture this bacterium for the production of vaccines.

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Unveiling the potential of systems biology in biotechnology and biomedical research

Saranya,

Thamanna,

Chellapandi

2024

Syst Microbiol and Biomanuf

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Genome-scale metabolic reconstruction for the insidious bacterium in aquaculture Piscirickettsia salmonis

Cited by 12 publications

References 42 publications

Analysis of Piscirickettsia salmonis Metabolism Using Genome-Scale Reconstruction, Modeling, and Testing

Analysis of Piscirickettsia salmonis Metabolism Using Genome-Scale Reconstruction, Modeling, and Testing

Engineering a defined culture medium to grow Piscirickettsia salmonis for its use in vaccine formulations

Unveiling the potential of systems biology in biotechnology and biomedical research

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