Genome scale portrait of cAMP-receptor protein (CRP) regulons in mycobacteria points to their role in pathogenesis

Akhter, Yusuf; Yellaboina, Sailu; Farhana, Aisha; Ranjan, Akash; Ahmed, Niyaz; Hasnain, Seyed E.

doi:10.1016/j.gene.2007.10.017

Cited by 45 publications

(46 citation statements)

References 42 publications

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“…Soil bacteria might have to deal with frequently fluctuating substrate levels so that they would need extra regulation rather than interacting with plants. Interestingly, since cAMP is also a subversion mechanism, some bacterial pathogens might also subvert plant cAMP production for their own benefit, through injection of adenylate cyclase and/or various toxins that alter adenylate cyclase levels (adenylate cyclase is essential to the production of cAMP) (Akhter et al, 2008;Agarwal and Bishai, 2009). Iron is an essential element for most organisms (Weinberg, 1984), but can be a limiting reagent for life (often in oceans, Boyd et al, 2007) owing to its insolubility in aerobic environments at neutral pH.…”

Section: Discussionmentioning

confidence: 99%

Structure, fluctuation and magnitude of a natural grassland soil metagenome

et al. 2012

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The soil ecosystem is critical for human health, affecting aspects of the environment from key agricultural and edaphic parameters to critical influence on climate change. Soil has more unknown biodiversity than any other ecosystem. We have applied diverse DNA extraction methods coupled with high throughput pyrosequencing to explore 4.88 Â 10 9 bp of metagenomic sequence data from the longest continually studied soil environment (Park Grass experiment at Rothamsted Research in the UK). Results emphasize important DNA extraction biases and unexpectedly low seasonal and vertical soil metagenomic functional class variations. Clustering-based subsystems and carbohydrate metabolism had the largest quantity of annotated reads assigned although o50% of reads were assigned at an E value cutoff of 10 À5. In addition, with the more detailed subsystems, cAMP signaling in bacteria (3.24±0.27% of the annotated reads) and the Ton and Tol transport systems (1.69±0.11%) were relatively highly represented. The most highly represented genome from the database was that for a Bradyrhizobium species. The metagenomic variance created by integrating natural and methodological fluctuations represents a global picture of the Rothamsted soil metagenome that can be used for specific questions and future inter-environmental metagenomic comparisons. However, only 1% of annotated sequences correspond to already sequenced genomes at 96% similarity and E values of o10 À5, thus, considerable genomic reconstructions efforts still have to be performed.

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Section: Discussionmentioning

confidence: 99%

Structure, fluctuation and magnitude of a natural grassland soil metagenome

et al. 2012

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“…paratuberculosis, and M. smegmatis (Akhter et al, 2008). Incorporation of a plasmid harboring and expressing M. tuberculosis Rv3676 in an M. tuberculosis strain in which Rv3676 was absent, induced differential expression of 27 genes when compared to the same mutant harboring the BCG gene orthologous to Rv3676 (Hunt et al, 2008).…”

Section: Crp Mt Regulonmentioning

confidence: 99%

“…Using an exponential enrichment (SELEX) approach, Bai et al defined the CRP Mt´s palindromic binding motif (C/TGTGANNNNNNT CACG/A) based on 58 predicted binding sites from the M. tuberculosis genome, using a combination of E. coli CRP binding sites and M. tuberculosis DNA sequences recovered by affinity capture using CRP Mt to seed the computational analyses (Bai et al, 2005). Akhter et al used the positional Shannon relative entropy method to predict 19 new putative binding sites for M. tuberculosis (Rv3676) CRP, in addition to the 73 sites previously predicted by Bai et al (Akhter et al, 2008, Bai et al, 2005. These additional sites resulted from a difference where Akhter et al used only the information available from the M. tuberculosis CRP-regulon instead of adding up the one available from the E.coli CRP-regulon.…”

Section: Crp Mt Regulonmentioning

confidence: 99%

“…These additional sites resulted from a difference where Akhter et al used only the information available from the M. tuberculosis CRP-regulon instead of adding up the one available from the E.coli CRP-regulon. According to Akhter et al, the M. tuberculosis CRPregulon comprises genes required for critical functions like: (i) cell-wall biogenesis, (ii) central metabolism pathways, (iii) putative regulatory elements controlling cAMP signaling, and (iv) antibiotic resistance (Akhter et al, 2008) (Table 2). …”

Section: Crp Mt Regulonmentioning

confidence: 99%

“…It is expected that specific cyclases are activated to modify cAMP levels in response to different physiological conditions as for example hypoxia, intramacrophage enviroment or pH changes. cAMP receptor protein (CRP) Rv3676 was found to exist as dimmer and exhibited cAMP binding in a concentration-dependent manner and could bind specifically to the putative CRP/FNR nucleotide sequence elements in response to hypoxia (Akhter et al, 2008). The protein itself is composed of three distinct regions of the polypeptide: a large Nterminal domain that binds cAMP, a long -helix (termed the C-helix) that mediates most of Table 3.…”

Section: Participation Of Camp In Mycobacterial Pathogenic Processesmentioning

confidence: 99%

See 2 more Smart Citations

Mycobacterium Tuberculosis Signaling via c-AMP

Flores-Valdéz¹,

Barba²,

Álvarez³

2012

Understanding Tuberculosis - Deciphering the Secret Life of the Bacilli

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Mycobacterium tuberculosis, as recent investigations demonstrate, has a complex signaling expression, which allows its close interaction with the environment and one of its most renowned properties: the ability to persist for long periods of time under a non-replicative status. Although this skill is well characterized in other bacteria, the intrinsically very slow growth rate of Mycobium tuberculosis, together with a very thick and complex cell wall, makes this pathogen specially adapted to the stress that could be generated by the host against them. In this book, different aspects of these properties are displayed by specialists in the field.

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Evolution of structural fitness and multifunctional aspects of mycobacterial RND family transporters

Sandhu

Akhter

2017

Arch Microbiol

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Drug resistance is a major concern due to the evolution and emergence of pathogenic bacterial strains with novel strategies to resist the antibiotics in use. Mycobacterium tuberculosis (Mtb) is one of such pathogens with reported strains, which are not treatable with any of the available anti-TB drugs. This scenario has led to the need to look for some novel drug targets in Mtb, which may be exploited to design effective treatment strategies against the infection. The goal of this review is to discuss one such class of emerging drug targets in Mtb. MmpL (mycobacterial membrane protein large) proteins from Mtb are reported to be involved in multi-substrate transport including drug efflux and considered as one of the contributing factors for the emergence of multidrug-resistant strains. MmpL proteins belong to resistance nodulation division permeases superfamily of membrane transporters, which are viably and pathogenetically important and their inhibition could be lethal for the bacteria.

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Genome scale portrait of cAMP-receptor protein (CRP) regulons in mycobacteria points to their role in pathogenesis

Cited by 45 publications

References 42 publications

Structure, fluctuation and magnitude of a natural grassland soil metagenome

Structure, fluctuation and magnitude of a natural grassland soil metagenome

Mycobacterium Tuberculosis Signaling via c-AMP

Evolution of structural fitness and multifunctional aspects of mycobacterial RND family transporters

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