Genome Sequence Analysis Reveals Evidence of Quorum-Sensing Genes Present in Aeromonas hydrophila Strain M062, Isolated from Freshwater

Chan, Kok-Gan; Tan, Wen-Si; Chang, Chien‐Yi; Yin, Wai‐Fong; Yunos, Nina Yusrina Mumahad

doi:10.1128/genomea.00100-15

Cited by 4 publications

(2 citation statements)

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“…Salmonicida (Vincent et al, 2017). This may not be surprising if we assume that the genomes of A. hydrophila used here have a similar GC% content to those previously published [60.2-61.3% (Chan et al, 2015;Tan et al, 2015a,b;Forn-Cuni et al, 2016a,b;Moura et al, 2017)] and that while bacteriophage genomes evolve to match the GC% of their hosts (Xia and Yuen, 2005), those whose GC content is lower may require tRNAs to complement their biochemical requirements. Finally, certain bacteriophages may code for and transfer ARGs in bacteria through transduction (Gunathilaka et al, 2017;Brown-Jaque et al, 2018;Larranaga et al, 2018;Wang et al, 2018).…”

Section: Discussionsupporting

confidence: 76%

“…However, bacteriophages in this study with a low GC% content and with a higher number of tRNAs had a lower biofilm disruptive efficacy. While we did not assess the GC% content of the A. hydrophila strains used for the biofilm assays here, the GC% of A. hydrophila published genomes ranged from 60.2 to 61.3% (Chan et al, 2015;Tan et al, 2015a,b;Forn-Cuni et al, 2016a,b;Moura et al, 2017). Assuming our bacterial strains had similar content, then it would appear that bacteriophages in our study which matched more closely the GC% of the bacteria were significantly more successful in degrading biofilms.…”

Section: Discussionmentioning

confidence: 99%

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Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

et al. 2020

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The increase in global warming has favored growth of a range of opportunistic environmental bacteria and allowed some of these to become more pathogenic to humans. Aeromonas hydrophila is one such organism. Surviving in moist conditions in temperate climates, these bacteria have been associated with a range of diseases in humans, and in systemic infections can cause mortality in up to 46% of cases. Their capacity to form biofilms, carry antibiotic resistance mechanisms, and survive disinfection, has meant that they are not easily treated with traditional methods. Bacteriophage offer a possible alternative approach for controlling their growth. This study is the first to report the isolation and characterization of bacteriophages lytic against clinical strains of A. hydrophila which carry intrinsic antibiotic resistance genes. Functionally, these novel bacteriophages were shown to be capable of disrupting biofilms caused by clinical isolates of A. hydrophila. The potential exists for these to be tested in clinical and environmental settings.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

et al. 2020

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Living in an Extremely Polluted Environment: Clues from the Genome of Melanin-Producing Aeromonas salmonicida subsp. pectinolytica 34mel ^T

Paván

Pavan

López

et al. 2015

Appl Environ Microbiol

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d Aeromonas salmonicida subsp. pectinolytica 34mel T can be considered an extremophile due to the characteristics of the heavily polluted river from which it was isolated. While four subspecies of A. salmonicida are known fish pathogens, 34melT belongs to the only subspecies isolated solely from the environment. Genome analysis revealed a high metabolic versatility, the capability to cope with diverse stress agents, and the lack of several virulence factors found in pathogenic Aeromonas. The most relevant phenotypic characteristics of 34mel T are pectin degradation, a distinctive trait of A. salmonicida subsp. pectinolytica, and melanin production. Genes coding for three pectate lyases were detected in a cluster, unique to this microorganism, that contains all genes needed for pectin degradation. Melanin synthesis in 34melT is hypothesized to occur through the homogentisate pathway, as no tyrosinases or laccases were detected and the homogentisate 1,2-dioxygenase gene is inactivated by a transposon insertion, leading to the accumulation of the melanin precursor homogentisate. Comparative genome analysis of other melanogenic Aeromonas strains revealed that this gene was inactivated by transposon insertions or point mutations, indicating that melanin biosynthesis in Aeromonas occurs through the homogentisate pathway. Horizontal gene transfer could have contributed to the adaptation of 34mel T to a highly polluted environment, as 13 genomic islands were identified in its genome, some of them containing genes coding for fitness-related traits. Heavy metal resistance genes were also found, along with others associated with oxidative and nitrosative stresses. These characteristics, together with melanin production and the ability to use different substrates, may explain the ability of this microorganism to live in an extremely polluted environment.A eromonas salmonicida subsp. pectinolytica 34melT is a melanin-producing Aeromonas strain that degrades polypectate, which is an unusual characteristic among Aeromonas species (1). It was isolated from the heavily polluted water of the Riachuelo, the last part of the Matanza River, located in Buenos Aires, Argentina. This river has received the effluents of hundreds of tanneries and other industries, as well as urban sewage and fuels, for more than a century. Among the contaminants found in this environment, which has high organic matter and low dissolved oxygen contents, are hydrocarbons, polychlorinated biphenyls, pesticides, arsenic, and heavy metals, such as chromium, lead, copper, mercury, and nickel (2-4). A molecular analysis of the microbial diversity of river water and sediments from the isolation site showed the presence of bacteria belonging to several taxa. Analysis of 16S rRNA gene sequences revealed the presence of bacteria belonging to the Beta-, Gamma-, and Epsilonproteobacteria in the water, as well as a higher level of diversity in the sediments, in which Alpha-, Beta-, Gamma-, and Deltaproteobacteria, Firmicutes, and Bacteroidetes were detected (5).The ge...

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Tad pili contribute to the virulence and biofilm formation of virulent Aeromonas hydrophila

Tekedar,

Patel,

Blom

et al. 2024

Front. Cell. Infect. Microbiol.

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Type IV pili (T4P) are versatile proteinaceous protrusions that mediate diverse bacterial processes, including adhesion, motility, and biofilm formation. Aeromonas hydrophila, a Gram-negative facultative anaerobe, causes disease in a wide range of hosts. Previously, we reported the presence of a unique Type IV class C pilus, known as tight adherence (Tad), in virulent Aeromonas hydrophila (vAh). In the present study, we sought to functionalize the role of Tad pili in the pathogenicity of A. hydrophila ML09-119. Through a comprehensive comparative genomics analysis of 170 A. hydrophila genomes, the conserved presence of the Tad operon in vAh isolates was confirmed, suggesting its potential contribution to pathogenicity. Herein, the entire Tad operon was knocked out from A. hydrophila ML09-119 to elucidate its specific role in A. hydrophila virulence. The absence of the Tad operon did not affect growth kinetics but significantly reduced virulence in catfish fingerlings, highlighting the essential role of the Tad operon during infection. Biofilm formation of A. hydrophila ML09-119 was significantly decreased in the Tad operon deletant. Absence of the Tad operon had no effect on sensitivity to other environmental stressors, including hydrogen peroxide, osmolarity, alkalinity, and temperature; however, it was more sensitive to low pH conditions. Scanning electron microscopy revealed that the Tad mutant had a rougher surface structure during log phase growth than the wildtype strain, indicating the absence of Tad impacts the outer surface of vAh during cell division, of which the biological consequences are unknown. These findings highlight the role of Tad in vAh pathogenesis and biofilm formation, signifying the importance of T4P in bacterial infections.

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Genome Sequence Analysis Reveals Evidence of Quorum-Sensing Genes Present in Aeromonas hydrophila Strain M062, Isolated from Freshwater

Abstract: Aeromonas hydrophila has emerged worldwide as a human pathogen. Here, we report the draft whole-genome sequence of a freshwater isolate from Malaysia, A. hydrophila strain M062, and its N-acylhomoserine lactone genes are also reported here.

Cited by 4 publications

References 10 publications

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

Living in an Extremely Polluted Environment: Clues from the Genome of Melanin-Producing Aeromonas salmonicida subsp. pectinolytica 34mel ^T

Tad pili contribute to the virulence and biofilm formation of virulent Aeromonas hydrophila

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Genome Sequence Analysis Reveals Evidence of Quorum-Sensing Genes Present in Aeromonas hydrophila Strain M062, Isolated from Freshwater

Abstract: Aeromonas hydrophila has emerged worldwide as a human pathogen. Here, we report the draft whole-genome sequence of a freshwater isolate from Malaysia, A. hydrophila strain M062, and its N-acylhomoserine lactone genes are also reported here.

Cited by 4 publications

References 10 publications

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

Living in an Extremely Polluted Environment: Clues from the Genome of Melanin-Producing Aeromonas salmonicida subsp. pectinolytica 34mel T

Tad pili contribute to the virulence and biofilm formation of virulent Aeromonas hydrophila

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Living in an Extremely Polluted Environment: Clues from the Genome of Melanin-Producing Aeromonas salmonicida subsp. pectinolytica 34mel ^T