Genome sequence data announcement of Bifidobacterium bifidum strain ICIS-202 isolated from a healthy human intestine stimulating active nitrogen oxide production in macrophages

Abstract

This report presents the data on the draft genome sequence of Bifidobacterium bifidum strain ICIS-202. The strain, isolated from the intestine of a young healthy woman, was deposited in the State Collection of Microorganisms of Normal Microbiota in Gabrichevsky Institute of Epidemiology and Microbiology, Moscow, Russian Federation as a prospective candidate for probiotic development. The size of the genome was 2,265,060 bp (62,4% G + C content). The annotation revealed 1771 coding sequences, including 1771 proteins, 5 rRNA, 52 tRNA, and 3 ncRNA genes. The draft genome sequence data of B. bifidum strain ICIS-202 is available in DBJ/EMBL/GenBank under the accession nos. SSMS00000000.1, PRJNA412271 and SAMN07709009 for Genome, Bioproject and Biosample databases, respectively.

Genome sequence data and properties of Bifidobacterium bifidum strain ICIS-504 isolated from multispecies bifidobacterial community

“…The studied strains were cultured in 4 ml of Schadler's broth (HiMedia Laboratories Pvt. Limited) for 48 h in an atmosphere of 0.6% oxygen and 5% carbon dioxide at 37 C, as described in [6] . DNA isolation, library preparation, sequencing, as well as genome assembly and annotation were performed in full accordance with the technique described in our previous work.…”

“…The peak area concentrations were calculated using GCsolution software (Shimadzu, Japan). To assess the immunoregulatory activity of bifidobacteria, we determined the ability of mononuclear cells isolated and incubated according to the method of previous work [6] to produce the indicated cytokines in concentrations measured by enzyme immunoassay using the method described in [7] .…”

Genome Sequence Data of Lactobacillus ruminis ICIS-540, Isolated from the Intestine of a Healthy Woman

“…Limited) for 24 hours at 0.9% O 2 and 9% CO 2 atmosphere and temperature of 37°C in a CO 2 incubator (Binder, Tuttlingen, Germany). The DNA extraction procedure was performed as described in our previous work ( 9 ). The obtained culture was centrifuged at 4,000 × g for 6 min, sediment was resuspended in 50 μl of Tris-buffered saline with 2 μg of lysozyme from chicken egg white, and the suspension was incubated at 37°C for 1 hour and then homogenized mechanically for 1 min by 1.4-mm silica beads at a 6.5 m/s speed.…”