Genome sequence heterogeneity of Lake Sinai Virus found in honey bees and Orf1/RdRP-based polymorphisms in a single host

Ravoet, Jorgen; Smet, Lina De; Wenseleers, Tom; Graaf, Dirk C. de

doi:10.1016/j.virusres.2015.02.019

Cited by 47 publications

(60 citation statements)

References 42 publications

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“…Partial genomes were recovered from all samples that shared approximately 70% identity to LSV1 and LSV2. Other LSV strains (LSV3–LSV7) with similar levels of divergence have also been identified from Europe and the USA 40 – 42 and from two previous Australian studies 43 , 44 . Only partial gene sequences are available for these variants, but high similarity was found between the QLD, VIC/NSW-1 and VIC/NSW-3 variants with Cairns isolates (94–97%) and the NT variant was 99% and 97% similar to LSV5 and LSV3 sequences.…”

Section: Resultssupporting

confidence: 59%

“…LSV1 and LSV2 are also new detections for Australia and extend the distribution of this virus complex. These viruses were identified as common viruses in the United States in 2009 and since then a diversity of LSV strains have been identified in Europe and North America 33 , 40 , 41 . With the detection of diverse Australian LSV strains it is apparent these viruses have had a long association with A. mellifera globally.…”

Section: Discussionmentioning

confidence: 99%

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Absence of deformed wing virus and Varroa destructor in Australia provides unique perspectives on honeybee viral landscapes and colony losses

Roberts

Anderson

Durr

2017

Sci Rep

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Honeybee (Apis mellifera) health is threatened globally by the complex interaction of multiple stressors, including the parasitic mite Varroa destructor and a number of pathogenic viruses. Australia provides a unique opportunity to study this pathogenic viral landscape in the absence of V. destructor. We analysed 1,240A. mellifera colonies across Australia by reverse transcription-polymerase chain reaction (RT-PCR) and next-generation sequencing (NGS). Five viruses were prevalent: black queen cell virus (BQCV), sacbrood virus (SBV), Israeli acute paralysis virus (IAPV) and the Lake Sinai viruses (LSV1 and LSV2), of which the latter three were detected for the first time in Australia. We also showed several viruses were absent in our sampling, including deformed wing virus (DWV) and slow bee paralysis virus (SBPV). Our findings highlight that viruses can be highly prevalent in A. mellifera populations independently of V. destructor. Placing these results in an international context, our results support the hypothesis that the co-pathogenic interaction of V. destructor and DWV is a key driver of increased colony losses, but additional stressors such as pesticides, poor nutrition, etc. may enable more severe and frequent colony losses to occur.

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Section: Resultssupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Absence of deformed wing virus and Varroa destructor in Australia provides unique perspectives on honeybee viral landscapes and colony losses

Roberts

Anderson

Durr

2017

Sci Rep

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“…It has hypothesised that all positivestrand RNA viruses could have emerged from a common ancestor virus that contained genes for RNA-dependent RNA polymerase, a protease that also acted as the capsid protein, and an RNA helicase. However, these gene regions were focused on by scientists for the purpose of the taxonomy of positive-sense RNA viruses, including honeybee viruses (Koonin et al 1993;Tapaszti et al 2009;Singh et al 2010;Mookhploy et al 2015;Ravoet et al 2015). The partial helicase gene region coding the helicase C domain and the partial capsid gene region were examined with regard to genetic divergence and molecular differences among the Turkish field viruses.…”

Section: Sequence Namementioning

confidence: 99%

“…Information regarding the nucleotide sequence and genetic classification of BQCV genotypes is globally very limited (Leat et al 2000;Benjeddou et al 2001;Tapaszti et al 2009;Singh et al 2010;Mookhploy et al 2015;Ravoet et al 2015). In the current study, the two viral genes were examined and the current results show that TrBQCVs highly conserved with each other and displayed a short genetic distance for the helicase gene (p value = 0.036) and the capsid gene (p value = 0.035).…”

Section: Sequence Namementioning

confidence: 99%

A molecular epidemiological study of black queen cell virus in honeybees (Apis mellifera) of Turkey: the first genetic characterization and phylogenetic analysis of field viruses

Muz

2017

Apidologie

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Black queen cell virus (BQCV) is one of the most common honeybee pathogens causing queen brood deaths. The 63 apiaries were sampled between 2007 and 2013 from four different ecogeographic regions in Turkey to estimate BQCV molecular structural characteristics. The BQCV positivity was 47.6%. The 25 local Black queen cell viruses (TrBQCVs) were molecularly characterized and investigated for their genetic relationship with previous records. The identity of the helicase gene among the TrBQCVs was 92-98%, whereas the similarity ranged from 37 to 85% in comparison with the intercontinental records. The identity of the partial capsid gene among the TrBQCVs was 91-100%, and the similarity rate varied from 86 to 97, 88-96, 90-97 and 89-99% in comparison with the Asian, African, American and European counterparts, respectively. The four nonsynonymous substitutions on the partial capsid protein suggest a predicted genotype that is specific among TrBQCVs.

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“…The spread of mites has facilitated the spread of viruses (Martin et al 2012) by acting as a viral reservoir and incubator (Gisder et al 2009). In addition, the mites' feeding behaviour allows viruses to be transmitted directly into the bees' haemolymph, thus bypassing established oral and sexual routes of transmission (Martin et al 2012;Amiri et al 2015;Ravoet et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of early spring bio-technical management techniques to control varroosis in Apis mellifera

2019

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More than 30 years after the first infestation report on Apis mellifera , varroa mites are still damaging honey bee health. The present paper reports data from a field experiment concerning two early spring (late February) bio-technical management techniques: early brood interruption (through queen caging) and early trapping comb (through the use of a single trapping comb). The aim of the study was to evaluate the impact of both techniques on colony development, mite population growth and honey production during the 6 months following the treatments. We observed that the early season queen handling and technical management of the colonies were able to affect mite reproduction resulting in a lower infestation rate, especially in the brood interruption group, while not causing any repercussion on the honey harvest and seasonal colony development and performance. varroa control / Varroa destructor / trapping comb / queen caging / bio-techniques Corresponding author: M. Lodesani,

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Genome sequence heterogeneity of Lake Sinai Virus found in honey bees and Orf1/RdRP-based polymorphisms in a single host

Cited by 47 publications

References 42 publications

Absence of deformed wing virus and Varroa destructor in Australia provides unique perspectives on honeybee viral landscapes and colony losses

Absence of deformed wing virus and Varroa destructor in Australia provides unique perspectives on honeybee viral landscapes and colony losses

A molecular epidemiological study of black queen cell virus in honeybees (Apis mellifera) of Turkey: the first genetic characterization and phylogenetic analysis of field viruses

Evaluation of early spring bio-technical management techniques to control varroosis in Apis mellifera

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