Genome Sequence of a Lethal Strain of Xylem-Invading
            <i>Verticillium nonalfalfae</i>

Jakše, Jernej; Jelen, Vid; Radišek, Sebastjan; Jonge, Ronnie de; Mandelc, Stanislav; Majer, Aljaz; Curk, Tomaž; Zupan, Blaž; Thomma, Bart P.H.J.; Javornik, Branka

doi:10.1128/genomea.01458-17

Cited by 13 publications

(19 citation statements)

References 12 publications

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“…Fungal pathogens have evolved diverse strategies to interact with host plants and secrete various effector molecules to overcome plant defense mechanisms. A recently published genome of xylem-invading Sordariomycete fungus Verticillium nonalfalfae [ 22 ], a transcriptome study of infected hop [ 45 ] and obtained proteomic data of fungal growth on xylem simulating medium [ 44 ] have provided an opportunity to screen for proteins that may contribute to fungal virulence in hop. In the current study, a customized bioinformatics pipeline was designed to predict the classical V .…”

Section: Discussionmentioning

confidence: 99%

“…Using a customized Genialis Platform (Genialis, Slovenia; https://www.genialis.com/genialis-platform/ ), the gene models of the V . nonalfalfae reference genome [ 22 ] were translated into putative proteins with the ExPASy Translate tool [ 100 ] and ORF Finder [ 101 ]. The general characteristics of putative proteins (molecular weight, number of amino acids (aa), percentage of cysteines and isoelectric point) were predicted by the ProtParam tool [ 100 ].…”

Section: Methodsmentioning

confidence: 99%

“…In the second step, the number of secreted proteins was narrowed down using the following criteria: proteins determined in the lethal pathotype-specific region identified by comparative genomics of mild and lethal V . nonalfalfae strains from three geographic regions [ 22 ]; proteins differentially expressed in lethal compared to mild V . nonalfalfae strains grown in xylem-simulating media as determined by RNA-Seq; V .…”

Section: Methodsmentioning

confidence: 99%

“…longisporum and V . nonalfalfae ) and their strains have been published [ 17 – 22 ], providing a wealth of genomic information for various studies. Klosterman et al [ 17 ] queried V .…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, a comprehensive biological database was generated using data from recently sequenced V . nonalfalfae genomes [ 22 ], transcriptomic and proteomic research of fungal growth on xylem stimulating medium [ 44 ] and RNA-seq studies of V . nonalfalfae –hop interactions [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Comprehensive analysis of Verticillium nonalfalfae in silico secretome uncovers putative effector proteins expressed during hop invasion

Marton¹,

Flajšman²,

Radišek³

et al. 2018

PLoS ONE

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The vascular plant pathogen Verticillium nonalfalfae causes Verticillium wilt in several important crops. VnaSSP4.2 was recently discovered as a V. nonalfalfae virulence effector protein in the xylem sap of infected hop. Here, we expanded our search for candidate secreted effector proteins (CSEPs) in the V. nonalfalfae predicted secretome using a bioinformatic pipeline built on V. nonalfalfae genome data, RNA-Seq and proteomic studies of the interaction with hop. The secretome, rich in carbohydrate active enzymes, proteases, redox proteins and proteins involved in secondary metabolism, cellular processing and signaling, includes 263 CSEPs. Several homologs of known fungal effectors (LysM, NLPs, Hce2, Cerato-platanins, Cyanovirin-N lectins, hydrophobins and CFEM domain containing proteins) and avirulence determinants in the PHI database (Avr-Pita1 and MgSM1) were found. The majority of CSEPs were non-annotated and were narrowed down to 44 top priority candidates based on their likelihood of being effectors. These were examined by spatio-temporal gene expression profiling of infected hop. Among the highest in planta expressed CSEPs, five deletion mutants were tested in pathogenicity assays. A deletion mutant of VnaUn.279, a lethal pathotype specific gene with sequence similarity to SAM-dependent methyltransferase (LaeA), had lower infectivity and showed highly reduced virulence, but no changes in morphology, fungal growth or conidiation were observed. Several putative secreted effector proteins that probably contribute to V. nonalfalfae colonization of hop were identified in this study. Among them, LaeA gene homolog was found to act as a potential novel virulence effector of V. nonalfalfae. The combined results will serve for future characterization of V. nonalfalfae effectors, which will advance our understanding of Verticillium wilt disease.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…longisporum and V . nonalfalfae ) and their strains have been published [ 17 – 22 ], providing a wealth of genomic information for various studies. Klosterman et al [ 17 ] queried V .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comprehensive analysis of Verticillium nonalfalfae in silico secretome uncovers putative effector proteins expressed during hop invasion

Marton¹,

Flajšman²,

Radišek³

et al. 2018

PLoS ONE

Self Cite

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show abstract

Verticillium dahliae and Verticillium nonalfalfae occurrence and abundance in several agricultural fields from Nova Scotia, Canada, assessed by real-time quantitative PCR

et al. 2019

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Confirming infection of hop plants inoculated with Verticilium nonalfalfae

2019

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Hop ( Humulus lupulus L.) is grown mostly as flavouring and bittering ingredient for beer and is also appreciated in the herbal and cosmetic industry, as well as in pharmacology. Among several diseases that damage hop growing, the most devastating in European hop production is verticillium wilt, caused by the soil-borne fungal pathogen Verticillium nonalfalfae . Colonization pattern and differential expression of selected genes after artificial infection of susceptible and resistant hop cultivars with V. nonalfalafae in stems and roots have been analysed recently Švara et al., 2019. Here, we present the dataset related to verification of plant samples infections after artificial inoculation (fungi- and mock-inoculated). After inoculation plant samples were tested for the positive infection by PCR amplification of the V. nonalfalfae ITS DNA region with species specific primers developed and optimised for this purpose. For more insight please see the article “Temporal and spatial assessment of defence responses in resistant and susceptible hop cultivars during infection with Verticillium nonalfalfae”.

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Genome Sequence of a Lethal Strain of Xylem-Invading Verticillium nonalfalfae

Cited by 13 publications

References 12 publications

Comprehensive analysis of Verticillium nonalfalfae in silico secretome uncovers putative effector proteins expressed during hop invasion

Comprehensive analysis of Verticillium nonalfalfae in silico secretome uncovers putative effector proteins expressed during hop invasion

Verticillium dahliae and Verticillium nonalfalfae occurrence and abundance in several agricultural fields from Nova Scotia, Canada, assessed by real-time quantitative PCR

Confirming infection of hop plants inoculated with Verticilium nonalfalfae

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