Genome sequence of a mallard duck origin cyclovirus, DuACyV-1

Abstract

The genome sequence of a novel avian cyclovirus is described in this study. The genome size and orientation of predicted genes was similar to those described in other vertebrate and insect origin cycloviruses. The greatest genome sequence identity was shared with a dragonfly cyclovirus (nt, 60.6%). Phylogenetic analysis showed marginal relatedness with another avian cyclovirus, the chicken associated cyclovirus 1. In contrast, along a short fragment of the replication-associated protein coding gene (rep) (spanning nt 1240-1710) the duck origin cyclovirus was very similar to human origin and honey bee origin rep sequences (human - TN4, 98%; honey bee - hb10, 100%). Related cyclovirus strains existing amongst various animal species living in diverse ecosystems and separated by large geographic distances show the need for additional studies to better understand the ecology and epidemiology of cycloviruses.

Occurrence and genetic diversity of CRESS DNA viruses in wild birds: a Hungarian study

“…A novel CRESS DNA virus, GgaCV-1, was uniquely identified in Hungary in two distantly related avian species, Eurasian teal and Eurasian jay 6 . Another newly described virus, DuACyV-1, since its first report 21 has been described in other waterfowls in Hungary and chickens in Brazil 22 . The question whether these two CRESS DNA viruses are pathogenic or innocuous viruses is open.…”

“…The DuACyV-1 genomes encoded cp on the viral strand and rep on the complementary strand, with a 158 nt long non-coding region (NCR) between the 5' ends and a four nt long NCR between the 3' ends of the two genes. The nonanucleotide motif sequence in the NCR upstream of the cp was TAGTATTAC for all DuACyV-1 genomes 21 (Fig. 2).…”

“…Therefore, we performed whole genome amplification and sequencing. Back-to-back PCR amplicons were generated for nine specimens not analyzed earlier 6,21 and then subjected to next generation sequencing (Table 1). Five, three and one sequences showed similarities with the genome of DuACyV-1, GoCV, and GgaCV-1, respectively.…”

Viral metagenomics revealed diverse CRESS-DNA virus genomes in faeces of forest musk deer

“…In the recent years, a large number of CRESS-DNA genomes have been determined in human and any other mammals, birds, insects, plants, fungi, and environment samples which bringing to light a high level of genetic diversity among these virus [25,26,31,33,52,56]. Although use metagenomics to identify these viruses from forest musk deer faeces, we cannot rule out that they may also represent food contaminants and environmental pollution [57].…”

“…[9], Smacoviridae [10], Geminiviridae, Nanoviridae and Bacilladnaviridae [11]. These are widely found in various environments [12,13], plant samples [14][15][16][17], dragonflies and damselflies [18][19][20][21], mosquitoes [22], rats [23], bats [24], duck [25], cattle [26], pigs [27,28], dogs [29], human [30][31][32][33], and turkey [34]. CRESS-DNA genomes typically encode a replication initiator protein (Rep) and a capsid protein (Cap) [35].…”